Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,237,642	IS5 (–) +4 bp	coding (109‑112/1011 nt)	mglC ←	D‑galactose/methyl‑galactoside ABC transporter membrane subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1397238	NA (NA)	49 (0.800)	36/278	0.5	100%	noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 2237645	0 (0.000)	49 (0.800)	36/278	0.5	100%	coding (109/1011 nt)	mglC	D‑galactose/methyl‑galactoside ABC transporter membrane subunit
*	?	NC_000913	2237642 =	0 (0.000)	64 (1.050)	43/276	0.2	100%	coding (112/1011 nt)	mglC	D‑galactose/methyl‑galactoside ABC transporter membrane subunit
*	?	NC_000913	= 2290113	NA (NA)	64 (1.050)	43/276	0.2	100%	noncoding (1/1195 nt)	IS5	repeat region

GGATAACGCCTTAAATGGCGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1397100‑1397238

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGGACCGATCTTTCTGCTGGTCGTCAGGTAGGGCTGGCG  <  NC_000913/2237645‑2237517

GGATAACGCCTTAAATGGCGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCACCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCT                                                                                                                                  >  2:431990/1‑140

  ATAACGCCTTAAATGGCGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTA                                                                                                                                >  1:93347/1‑140

   TAACGCCTTAAATGGCGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAA                                                                                                                               <  1:784882/140‑1

    AACGCCTTAAATGGCGAAGAAACGGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAA                                                                                                                               <  2:237236/140‑1

     ACGCCTTAAATGGCGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGT                                                                                                                             <  2:631464/140‑1

             AATGGCGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAA                                                                                                                     <  2:82426/140‑1

              ATGGCGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAAC                                                                                                                    <  2:353534/140‑1

                GGCGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTT                                                                                                                  <  2:875507/140‑1

                GGCGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTT                                                                                                                  <  1:294825/140‑1

                GGCGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTT                                                                                                                  <  1:352390/140‑1

                 GCGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTA                                                                                                                 >  2:114097/1‑140

                  CGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAA                                                                                                                >  1:322389/1‑140

                          C‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATT                                                                                                        <  2:791745/140‑1

                                       GCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCA                                                                                            >  1:427300/1‑140

                                        CTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCAT                                                                                           <  2:916764/140‑1

                                        CTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCAT                                                                                           >  1:685106/1‑140

                                         TGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATC                                                                                          <  2:439715/140‑1

                                         TGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATC                                                                                          <  2:565408/140‑1

                                          GATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCG                                                                                         >  1:964407/1‑140

                                          GATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCG                                                                                         <  2:994383/140‑1

                                          GATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCG                                                                                         >  1:328511/1‑140

                                          GATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAATAATATTCTCACCCAGTCATCG                                                                                         <  1:148738/140‑1

                                            TTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGT                                                                                       <  2:1005659/140‑1

                                            TTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGT                                                                                       >  1:630695/1‑140

                                             TCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTG                                                                                      <  1:139898/140‑1

                                             TCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTG                                                                                      <  1:145505/140‑1

                                             TCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTG                                                                                      <  2:638664/140‑1

                                               AAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCG                                                                                    <  2:604014/140‑1

                                               AAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCG                                                                                    <  1:795089/140‑1

                                                 GGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTA                                                                                  >  1:946330/1‑140

                                                   CATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATT                                                                                <  2:886111/140‑1

                                                    ATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTA                                                                               <  2:787338/140‑1

                                                            GAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCG                                                                       <  1:834705/140‑1

                                                                          TCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCGCCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGG                                                         <  2:805915/140‑1

                                                                                    AGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTC                                               <  2:427300/140‑1

                                                                                        ATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCC                                           >  2:421314/1‑140

                                                                                        ATGAAATGACTGAGACAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGCCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCC                                           >  2:868085/1‑140

                                                                                             ATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGG                                      <  2:946330/140‑1

                                                                                               GACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGGAC                                    <  2:677319/140‑1

                                                                                                    AGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGGACCGATC                               >  2:489592/1‑140

                                                                                                          CCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGGACCGATCTTTCTG                         >  1:893564/1‑140

                                                                                                            GAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGGACCGATCTTTCTGCT                       <  1:612884/140‑1

                                                                                                               AAGAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGGTTATTGTCACCCAGGGGACCGATCTTTCTGCTGGa                    >  2:406872/1‑139

                                                                                                                 GAATTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGATCTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGGACCGATCTTTCTGCTGGTCG                  <  1:699178/140‑1

                                                                                                                    TTTCCCCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGGACCGATCTTTCTGCTGGTCGTCA               <  1:421314/140‑1

                                                                                                                         CCGCTTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGGACCGATCTTTCTGCTGGTCGTCAGGTAG          >  2:872418/1‑140

                                                                                                                             TTATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGGACCGATCTTTCTGCTGGTCGTCAGGTAGGGCT      <  2:381463/140‑1

                                                                                                                              TATTCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGGACCGATCTTTCTGCTGGTCGTCAGGTAGGGCTG     <  1:329008/140‑1

                                                                                                                                 TCGCACCTTCCTTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGGACCGATCTTTCTGCTGGTCGTCAGGTAGGGCTGGCG  >  1:656094/1‑140

GGATAACGCCTTAAATGGCGAAGAAAC‑GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1397100‑1397238

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAAGTCTGTTGAACTTAAGTAATATTCTCACCCAGTCATCGGTGCGTATTATTATCGCGCTCGGTGTGGCAGGGTTAATTGTCACCCAGGGGACCGATCTTTCTGCTGGTCGTCAGGTAGGGCTGGCG  <  NC_000913/2237645‑2237517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A