Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 132 61.4 2079690 99.1% 2060972 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,720,390 A→G K146K (AAA→AAG)  patZ → peptidyl‑lysine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,720,3900AG100.0% 177.5 / NA 47K146K (AAA→AAG) patZpeptidyl‑lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/26);  total (21/26)

CGGCATCGCAACACGAAGATCTCCGCGCCTGCGCCCTGCGCCATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTG  >  NC_000913/2720260‑2720525
                                                                                                                                  |                                                                                                                                       
cGGCATCGCAACACGAAGATCTCCGCGCCTGCGCCCTGCGCCATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAg                                                                                                                                <  2:859572/140‑1 (MQ=255)
cGGCATCGCAACACGAAGATCTCCGCGCCTGCGCCCTGCGCCATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAg                                                                                                                                <  2:59347/140‑1 (MQ=255)
 ggCATCGCAACACGAAGATCTCCGCGCCTGCGCCCTGCGCCATAACATGCGCCTGCTTGGACCAAACGGCCGGGGTTTCCTGGCCCCCGGGCAGGGCCTGAAGCCCGCCTTTTCGCCTGGCCCGATTAACGCCGGCAAGc                                                                                                                               >  2:637666/1‑140 (MQ=255)
           cacGAAGATCTCCGCGCCTGCGCCCTGCGCCATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATT‑AGCGCGGCAAGCTGGCGTTTAtt                                                                                                                    <  2:319732/140‑1 (MQ=255)
            acGAAGATCTCCGCGCCTGCGCCCTGCGCCATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTAtt                                                                                                                    <  2:8235/140‑1 (MQ=255)
                   tctcCGCGCCTGCGCCCTGCGCCATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAAt                                                                                                             >  1:352523/1‑140 (MQ=255)
                      ccGCGCCTGCGCCCTGCGCCATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCgg                                                                                                          >  1:283743/1‑140 (MQ=255)
                         cgcCTGCGCCCTGCGCCATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTg                                                                                                       <  2:844348/140‑1 (MQ=255)
                            ctgcgccctgcgccATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCg                                                                                                    <  2:486185/140‑1 (MQ=255)
                              gcgccctgcgccATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtc                                                                                                  >  1:359150/1‑140 (MQ=255)
                                     gcgccATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAaca                                                                                           <  1:80530/140‑1 (MQ=255)
                                     gcgccATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAaca                                                                                           <  2:310612/140‑1 (MQ=255)
                                      cgccATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAacac                                                                                          >  1:485695/1‑140 (MQ=255)
                                           tAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATcc                                                                                     >  1:454688/1‑140 (MQ=255)
                                               aTGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGa                                                                                 <  1:630341/140‑1 (MQ=255)
                                                tGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGAc                                                                                >  1:301150/1‑140 (MQ=255)
                                                        cTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCa                                                                        >  2:32851/1‑140 (MQ=255)
                                                         ttGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCaa                                                                       <  1:139116/140‑1 (MQ=255)
                                                            gACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAg                                                                    <  1:490138/140‑1 (MQ=255)
                                                              ccAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCg                                                                  <  2:283743/140‑1 (MQ=255)
                                                                 aaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTaa                                                               >  2:621609/1‑140 (MQ=255)
                                                                 aaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTaa                                                               >  1:863570/1‑140 (MQ=255)
                                                                 aaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTaa                                                               >  1:603791/1‑140 (MQ=255)
                                                                  aCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAg                                                              >  2:669410/1‑140 (MQ=255)
                                                                      tCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATgg                                                          <  1:913901/140‑1 (MQ=255)
                                                                        tGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGc                                                        <  2:1029415/140‑1 (MQ=255)
                                                                         gggTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCt                                                       <  2:454688/140‑1 (MQ=255)
                                                                         gggTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCt                                                       <  1:637666/140‑1 (MQ=255)
                                                                           gTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCttt                                                     <  2:653725/140‑1 (MQ=255)
                                                                              tACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTcc                                                  >  1:892949/1‑140 (MQ=255)
                                                                                cTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTa                                                >  2:1034672/1‑140 (MQ=255)
                                                                                cTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTa                                                >  1:440852/1‑140 (MQ=255)
                                                                                cTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTa                                                >  2:496714/1‑140 (MQ=255)
                                                                                    cTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACttt                                            >  1:703237/1‑140 (MQ=255)
                                                                                               ggTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCgg                                 >  2:843603/1‑140 (MQ=255)
                                                                                                 tCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCg                               <  1:584561/140‑1 (MQ=255)
                                                                                                 tCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCg                               >  2:355329/1‑140 (MQ=255)
                                                                                                        gCCAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCt                        <  1:669410/140‑1 (MQ=255)
                                                                                                         ccAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTg                       <  2:301150/140‑1 (MQ=255)
                                                                                                          cAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTgg                      >  1:632713/1‑140 (MQ=255)
                                                                                                          cAGCTTTTCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTgg                      >  2:810049/1‑140 (MQ=255)
                                                                                                                 tCGCCTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGa               <  1:123471/140‑1 (MQ=255)
                                                                                                                     cTGTGCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGtt           <  1:355329/140‑1 (MQ=255)
                                                                                                                       gtgCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtga         >  2:311258/1‑140 (MQ=255)
                                                                                                                         gCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatg       <  1:810049/140‑1 (MQ=255)
                                                                                                                         gCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatg       <  1:188647/140‑1 (MQ=255)
                                                                                                                         gCCGATTAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatg       <  2:863570/140‑1 (MQ=255)
                                                                                                                              ttAAGCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTg  <  2:441756/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                       
CGGCATCGCAACACGAAGATCTCCGCGCCTGCGCCCTGCGCCATAACATGCGCCTGCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTG  >  NC_000913/2720260‑2720525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A