Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,975,132	T→C	T295A (ACC→GCC)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,975,132	0	T	C	100.0%	145.9 / NA	41	T295A (ACC→GCC)	aas	acyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/24); total (17/24)

ACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTT  >  NC_000913/2974996‑2975267

aCATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAg                                                                                                                                      <  2:354857/140‑1 (MQ=255)

     tGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCtt                                                                                                                                 >  1:741719/1‑140 (MQ=255)

      gCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCttt                                                                                                                                <  2:167395/140‑1 (MQ=255)

       cTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCtttt                                                                                                                               >  2:388404/1‑140 (MQ=255)

           tAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTAccc                                                                                                                           <  1:746302/140‑1 (MQ=255)

            aaaTCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTAcccc                                                                                                                          >  1:189415/1‑140 (MQ=255)

              aTCTTCCAGATAGACCCGGCGCCCCTGAGTGAGTTGCTCGGGCAGAGGCCAGATTTTGCCTTTATCGAGAAACTGGGGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCgg                                                                                                                        <  2:419438/140‑1 (MQ=255)

              aTCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCgg                                                                                                                        <  2:355554/140‑1 (MQ=255)

                 ttCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAg                                                                                                                     <  1:742044/140‑1 (MQ=255)

                 ttCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAg                                                                                                                     >  2:471546/1‑140 (MQ=255)

                  tCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAgt                                                                                                                    >  1:529704/1‑140 (MQ=255)

                   ccAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAgtg                                                                                                                   <  2:877182/140‑1 (MQ=255)

                   ccAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAgtg                                                                                                                   <  1:814840/140‑1 (MQ=255)

                   ccAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAgtg                                                                                                                   <  1:404708/140‑1 (MQ=255)

                       aTAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGt                                                                                                               >  2:310676/1‑140 (MQ=255)

                           aCCCAGCGCACCTGAGTAAGTTGCTCCGACAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAAAAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTtcat                                                                                                           >  1:770767/1‑140 (MQ=255)

                                 cgcACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGc                                                                                                     >  1:170393/1‑140 (MQ=255)

                                       tGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCACCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCAt                                                                                               <  2:345872/140‑1 (MQ=255)

                                                tGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAGATGGTTTTGATTTTAGCCGCCGTAATAGCACTGGGCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCCGCGCCg                                                                                      >  2:299679/1‑140 (MQ=255)

                                                  cTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACggg                                                                                    >  2:663959/1‑140 (MQ=255)

                                                    ccGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCg                                                                                  >  1:548079/1‑140 (MQ=255)

                                                    ccGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCg                                                                                  <  2:189415/140‑1 (MQ=255)

                                                            tGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGcccc                                                                          >  2:910863/1‑140 (MQ=255)

                                                            tGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGcccc                                                                          >  1:791507/1‑140 (MQ=255)

                                                                    tttGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCa                                                                  <  2:1011150/140‑1 (MQ=255)

                                                                       gCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTCATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCTATGGCCCCAAAAATCACTg                                                               >  1:991981/1‑140 (MQ=255)

                                                                              tCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACt                                                        >  2:745763/1‑140 (MQ=255)

                                                                              tCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACt                                                        <  1:471546/140‑1 (MQ=255)

                                                                                   aaaCTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGc                                                   <  2:840752/140‑1 (MQ=255)

                                                                                    aaCTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGcc                                                  >  2:756446/1‑140 (MQ=255)

                                                                                        ggCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCa                                              <  2:33430/140‑1 (MQ=255)

                                                                                             gAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTggg                                         <  1:927343/140‑1 (MQ=255)

                                                                                              aaGTGAAGATGTTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGgc                                        <  2:741719/140‑1 (MQ=255)

                                                                                                          ttttCATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCTATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGACg                            <  2:991981/140‑1 (MQ=255)

                                                                                                           tttGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGa                           <  1:388404/140‑1 (MQ=255)

                                                                                                                tttCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGt                      <  1:387658/140‑1 (MQ=255)

                                                                                                                  tCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTc                    <  2:464033/140‑1 (MQ=255)

                                                                                                                  tCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTc                    <  1:910863/140‑1 (MQ=255)

                                                                                                                                 gCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTa     <  1:956269/140‑1 (MQ=255)

                                                                                                                                 gCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTa     <  2:74583/140‑1 (MQ=255)

                                                                                                                                    cTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTAttt  >  1:120941/1‑140 (MQ=255)

                                                                                                                                    cTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTAttt  >  1:103304/1‑140 (MQ=255)

ACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTT  >  NC_000913/2974996‑2975267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A