Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,007,752	T→C	G81G (GGT→GGC)	ygeX →	2,3‑diaminopropionate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,007,752	0	T	C	100.0%	154.7 / NA	41	G81G (GGT→GGC)	ygeX	2,3‑diaminopropionate ammonia‑lyase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/22); total (19/22)

AATAGCTGGTTATCGCCCAACACCGCTTTGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGT  >  NC_000913/3007623‑3007887

aaTAGCTGGTTATCGCCCAACACCGCTTTGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCa                                                                                                                               <  2:997318/140‑1 (MQ=255)

 aTAGCTGGTTATCGCCCAACACCGCTTTGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCAt                                                                                                                              <  2:824327/140‑1 (MQ=255)

 aTAGCTGGTTATCGCCCAACACCGCTTTGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCAt                                                                                                                              <  1:66926/140‑1 (MQ=255)

  tAGCTGGTTATCGCCCAACACCGCTTTGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAGACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATc                                                                                                                             <  2:546386/140‑1 (MQ=255)

   aGCTGGTTATCGCCCAACACCGCTTTGTGCGCTGGACGCTCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCg                                                                                                                            <  2:440614/140‑1 (MQ=255)

    gCTGGTTATCGCCCAACACCGCTTTGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGc                                                                                                                           >  2:940186/1‑140 (MQ=255)

          tATCGCCCAACACCGCTTTGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAatt                                                                                                                     >  1:199148/1‑140 (MQ=255)

                  aaCACCGCTTTGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATtgtgtg                                                                                                             >  2:575669/1‑140 (MQ=255)

                   acacCGCTTTGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGa                                                                                                            <  2:412060/140‑1 (MQ=255)

                   acacCGCTTTGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGa                                                                                                            <  1:7424/140‑1 (MQ=255)

                          tttGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGATTCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAatat                                                                                                     >  2:216606/1‑140 (MQ=255)

                          tttGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAatat                                                                                                     <  2:386877/140‑1 (MQ=255)

                           ttGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATatc                                                                                                    <  1:940186/140‑1 (MQ=255)

                              tgCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATatcatc                                                                                                 >  1:1023396/1‑140 (MQ=255)

                                  cTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGa                                                                                             <  1:874833/140‑1 (MQ=255)

                                     gACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGatat                                                                                          >  2:614741/1‑140 (MQ=255)

                                        gATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTAGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGa                                                                                       >  1:889428/1‑140 (MQ=255)

                                          tctcGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGaaa                                                                                     >  2:878393/1‑140 (MQ=255)

                                          tctcGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGaaa                                                                                     >  1:534065/1‑140 (MQ=255)

                                           ctcGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAAc                                                                                    <  1:847913/140‑1 (MQ=255)

                                                   ccTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCAt                                                                            <  1:688005/140‑1 (MQ=255)

                                                      ttttGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTg                                                                         >  1:970451/1‑140 (MQ=255)

                                                      ttttGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTg                                                                         >  1:624507/1‑140 (MQ=255)

                                                              tgAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTg                                                                 >  2:900284/1‑140 (MQ=255)

                                                              tgAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTg                                                                 <  2:13900/140‑1 (MQ=255)

                                                              tgAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTg                                                                 >  1:595474/1‑140 (MQ=255)

                                                                aagaaAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGaa                                                               >  2:339590/1‑140 (MQ=255)

                                                                          tcGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATc                                                     >  1:168650/1‑140 (MQ=255)

                                                                            gTCAAAGACGATTCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCgg                                                   <  1:216606/140‑1 (MQ=255)

                                                                                      aaTCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATg                                         >  2:766522/1‑140 (MQ=255)

                                                                                       aTCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGa                                        >  1:867263/1‑140 (MQ=255)

                                                                                          aaaaCGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACtt                                     <  2:1023396/140‑1 (MQ=255)

                                                                                            aaCGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTc                                   <  1:878393/140‑1 (MQ=255)

                                                                                               gATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTcgcg                                <  1:642445/140‑1 (MQ=255)

                                                                                                      tCTGAACGCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGaccacca                         <  1:853793/140‑1 (MQ=255)

                                                                                                             gCCTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACgg                  <  2:18368/140‑1 (MQ=255)

                                                                                                               cTTCAAAATGCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCa                >  1:259250/1‑140 (MQ=255)

                                                                                                                        gCTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGgc       <  1:900284/140‑1 (MQ=255)

                                                                                                                         cTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGgcg      >  1:656589/1‑140 (MQ=255)

                                                                                                                         cTTGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGgcg      <  1:478937/140‑1 (MQ=255)

                                                                                                                           tGGCGGCGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGcgcg    <  2:595474/140‑1 (MQ=255)

                                                                                                                             gcggcgCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGGTGTCATTTGAGCACCTGAAAAATGCCACCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGgt  <  2:281349/140‑1 (MQ=255)

AATAGCTGGTTATCGCCCAACACCGCTTTGTGCGCTGGACGATCTCGCAAACCTTTTTGGTGTGAAGAAAATTCTCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGT  >  NC_000913/3007623‑3007887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A