Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,070,755	G→A	R164C (CGC→TGC)	fbaA ←	fructose‑bisphosphate aldolase class II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,070,755	0	G	A	100.0%	189.9 / NA	57	R164C (CGC→TGC)	fbaA	fructose‑bisphosphate aldolase class II
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (36/21); total (36/21)

TCGGTGTATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCAC  >  NC_000913/3070619‑3070891

tCGGTGTATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTc                                                                                                                                       <  1:140631/140‑1 (MQ=255)

              aTCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGc                                                                                                                         <  1:727925/140‑1 (MQ=255)

              aTCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGc                                                                                                                         >  2:326451/1‑140 (MQ=255)

                  aCGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGAt                                                                                                                     >  1:1006757/1‑140 (MQ=255)

                       ttCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGa                                                                                                                <  2:5375/140‑1 (MQ=255)

                         ccGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATg                                                                                                              <  1:813532/140‑1 (MQ=255)

                         ccGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATg                                                                                                              <  1:373647/140‑1 (MQ=255)

                                ggTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCtt                                                                                                       >  1:1001637/1‑140 (MQ=255)

                                      cAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCagag                                                                                                 <  1:210626/140‑1 (MQ=255)

                                       aGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAgaga                                                                                                <  1:466593/140‑1 (MQ=255)

                                         tGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAtt                                                                                              >  2:227031/1‑140 (MQ=255)

                                         tGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAtt                                                                                              >  2:599760/1‑140 (MQ=255)

                                          gCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttc                                                                                             >  2:843172/1‑140 (MQ=255)

                                          gCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttc                                                                                             >  1:66765/1‑140 (MQ=255)

                                           cAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttct                                                                                            >  2:217020/1‑140 (MQ=255)

                                           cAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttct                                                                                            >  2:180857/1‑140 (MQ=255)

                                           cAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttct                                                                                            >  1:983707/1‑140 (MQ=255)

                                           cAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttct                                                                                            >  1:970044/1‑140 (MQ=255)

                                           cAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttct                                                                                            >  2:727659/1‑140 (MQ=255)

                                                   tCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAg                                                                                    >  1:78292/1‑140 (MQ=255)

                                                   tCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAg                                                                                    >  1:188903/1‑140 (MQ=255)

                                                   tCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAg                                                                                    >  1:218209/1‑140 (MQ=255)

                                                   tCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAg                                                                                    >  1:952762/1‑140 (MQ=255)

                                                        gtgGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTA‑TTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGAt                                                                              <  2:453696/140‑1 (MQ=255)

                                                         tgGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGAt                                                                              <  1:775015/140‑1 (MQ=255)

                                                              gttgtCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCAtgt                                                                         >  1:78831/1‑140 (MQ=255)

                                                                 gtCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTgag                                                                      >  1:944554/1‑140 (MQ=255)

                                                                 gtCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTgag                                                                      >  1:317340/1‑140 (MQ=255)

                                                                 gtCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGAATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTgag                                                                      >  2:331084/1‑140 (MQ=255)

                                                                                   accaccGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTAc                                                    <  1:180857/140‑1 (MQ=255)

                                                                                      accGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCgg                                                 <  2:970044/140‑1 (MQ=255)

                                                                                            gCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTg                                           >  2:605324/1‑140 (MQ=255)

                                                                                               aCCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGa                                        >  1:17247/1‑140 (MQ=255)

                                                                                               aCCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGa                                        >  2:709138/1‑140 (MQ=255)

                                                                                                 ccAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAg                                      <  2:186760/140‑1 (MQ=255)

                                                                                                 ccAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAg                                      <  2:95536/140‑1 (MQ=255)

                                                                                                  cAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAgt                                     >  2:755821/1‑140 (MQ=255)

                                                                                                   aGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAgtg                                    <  1:599760/140‑1 (MQ=255)

                                                                                                     ttCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtt                                  >  1:652353/1‑140 (MQ=255)

                                                                                                     ttCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtt                                  >  2:37350/1‑140 (MQ=255)

                                                                                                     ttCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtt                                  >  2:442191/1‑140 (MQ=255)

                                                                                                     ttCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtt                                  >  2:936670/1‑140 (MQ=255)

                                                                                                     ttCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtt                                  >  1:435550/1‑140 (MQ=255)

                                                                                                     ttCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtt                                  >  1:418443/1‑140 (MQ=255)

                                                                                                           ttCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCAc                            <  1:612313/140‑1 (MQ=255)

                                                                                                             ccAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCAccc                          <  2:78292/140‑1 (MQ=255)

                                                                                                                  gTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTc                     >  1:220065/1‑140 (MQ=255)

                                                                                                                  gTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTc                     <  2:66765/140‑1 (MQ=255)

                                                                                                                   tCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTcc                    >  2:636075/1‑140 (MQ=255)

                                                                                                                   tCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTcc                    >  2:208038/1‑140 (MQ=255)

                                                                                                                      tGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAAc                 >  2:705225/1‑140 (MQ=255)

                                                                                                                             tttGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGt          <  1:331084/140‑1 (MQ=255)

                                                                                                                             tttGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGt          <  2:1006757/140‑1 (MQ=255)

                                                                                                                                 gACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGAt      >  2:109143/1‑140 (MQ=255)

                                                                                                                                     tGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCAc  <  2:571164/140‑1 (MQ=255)

                                                                                                                                     tGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCAc  >  1:847652/1‑140 (MQ=255)

                                                                                                                                     tGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCAc  <  2:17247/140‑1 (MQ=255)

TCGGTGTATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCAC  >  NC_000913/3070619‑3070891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A