Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,174,743	A→G	G218G (GGT→GGC)	parE ←	DNA topoisomerase IV subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,174,743	0	A	G	100.0%	102.9 / NA	28	G218G (GGT→GGC)	parE	DNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/13); total (15/13)

CACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGT  >  NC_000913/3174616‑3174873

cACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAg                                                                                                                        <  2:226440/140‑1 (MQ=255)

   cGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGCAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGCCCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCAc                                                                                                                     <  2:1023846/140‑1 (MQ=255)

   cGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCAc                                                                                                                     <  2:750752/140‑1 (MQ=255)

        tCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCg                                                                                                                <  2:52614/140‑1 (MQ=255)

        tCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCg                                                                                                                <  2:730836/140‑1 (MQ=255)

        tCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCg                                                                                                                >  2:328217/1‑140 (MQ=255)

        tCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCg                                                                                                                >  2:122345/1‑140 (MQ=255)

           ggCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCgttg                                                                                                             <  2:691363/140‑1 (MQ=255)

                  acaGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTa                                                                                                      >  2:64900/1‑140 (MQ=255)

                  acaGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTa                                                                                                      <  2:800942/140‑1 (MQ=255)

                  acaGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTa                                                                                                      <  2:442431/140‑1 (MQ=255)

                  acaGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTa                                                                                                      <  2:474236/140‑1 (MQ=255)

                     gTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTGCCGATAAACGGTATTTCCGGCAGCGTCGGCAGACCTTTTACCGCGTCCGCCATGTAATCATACAGGCCGACCTGATAGCACCAGCGTTGTTCGGTAttg                                                                                                   <  2:700063/140‑1 (MQ=255)

                       aGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGTGTTGTTCGGTAttgtt                                                                                                 >  2:486105/1‑140 (MQ=255)

                          gcCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGAGAAACGGATATTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCAAGGAAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGAt                                                                                              >  2:486054/1‑140 (MQ=255)

                                tCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATc                                                                                        >  1:925638/1‑140 (MQ=255)

                                tCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATc                                                                                        >  2:142605/1‑140 (MQ=255)

                                      gCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTaaa                                                                                  >  1:913462/1‑140 (MQ=255)

                                          cAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTg                                                                              >  2:562911/1‑140 (MQ=255)

                                                     gCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGcc                                                                   >  1:1021358/1‑140 (MQ=255)

                                                         aaTTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAggg                                                               <  2:621249/140‑1 (MQ=255)

                                                           ttACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGca                                                             >  1:757441/1‑140 (MQ=255)

                                                               cGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAAt                                                         >  1:607305/1‑140 (MQ=255)

                                                                gATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATa                                                        >  2:349147/1‑140 (MQ=255)

                                                                        gTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTg                                                <  1:898622/140‑1 (MQ=255)

                                                                                  cAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGca                                      <  2:871350/140‑1 (MQ=255)

                                                                                                                  ggTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCggg      <  2:524692/140‑1 (MQ=255)

                                                                                                                  ggTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCggg      <  1:495010/140‑1 (MQ=255)

                                                                                                                   ttAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGc     >  1:175583/2‑140 (MQ=255)

                                                                                                                   gTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGc     >  2:529288/1‑140 (MQ=255)

                                                                                                                      aTCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGt  >  1:549393/1‑140 (MQ=255)

CACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGT  >  NC_000913/3174616‑3174873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A