Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,591,066	G→A	T420T (ACC→ACT)	ugpB ←	sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,591,066	0	G	A	100.0%	158.5 / NA	46	T420T (ACC→ACT)	ugpB	sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/22); total (24/22)

TGTGCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCGGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGCCCTTTGGTGAACGGCAACGGCGGCTT  >  NC_000913/3590930‑3591191

tgtgCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTa                                                                                                                            >  2:481706/1‑140 (MQ=255)

  tgCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATc                                                                                                                          >  1:749805/1‑140 (MQ=255)

  tgCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATc                                                                                                                          >  1:733104/1‑140 (MQ=255)

  tgCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATc                                                                                                                          >  1:207507/1‑140 (MQ=255)

  tgCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATc                                                                                                                          >  1:26592/1‑140 (MQ=255)

  tgCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATc                                                                                                                          >  2:508466/1‑140 (MQ=255)

      ggATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGt                                                                                                                      <  1:883080/140‑1 (MQ=255)

        aTGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGc                                                                                                                    <  2:783668/140‑1 (MQ=255)

        aTGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGc                                                                                                                    <  2:749805/140‑1 (MQ=255)

         tGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGcc                                                                                                                   <  2:689789/140‑1 (MQ=255)

           cgcttcgcttATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCctg                                                                                                                 <  1:10901/140‑1 (MQ=255)

                             tACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCCCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTAcc                                                                                               <  2:908325/140‑1 (MQ=255)

                               cacGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCgg                                                                                             >  1:75217/1‑140 (MQ=255)

                                     aTGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCcaca                                                                                       >  1:235573/1‑140 (MQ=255)

                                       gCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACg                                                                                     <  2:207507/140‑1 (MQ=255)

                                              cAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCa                                                                              <  2:973563/140‑1 (MQ=255)

                                                gATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGc                                                                            >  1:436849/1‑140 (MQ=255)

                                                 aTAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGct                                                                           >  1:287481/1‑140 (MQ=255)

                                                   aaGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGctct                                                                         <  2:131894/140‑1 (MQ=255)

                                                    aGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCtt                                                                        <  2:235573/140‑1 (MQ=255)

                                                     gCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTc                                                                       >  1:591013/1‑140 (MQ=255)

                                                               tCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCa                                                                              >  1:459346/1‑123 (MQ=255)

                                                               tCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCa                                                                              <  2:459346/123‑1 (MQ=255)

                                                               tCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATc                                                             >  1:507048/1‑140 (MQ=255)

                                                                  gACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCAcg                                                          <  2:733104/140‑1 (MQ=255)

                                                                     aTTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCgg                                                       >  1:681241/1‑140 (MQ=255)

                                                                     aTTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCgg                                                       >  1:867622/1‑140 (MQ=255)

                                                                       tACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATTACGCGGAt                                                     <  1:508466/140‑1 (MQ=255)

                                                                          acTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTg                                                  >  2:473830/1‑140 (MQ=255)

                                                                              aTTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGc                                              >  1:637788/1‑140 (MQ=255)

                                                                               ttAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCa                                             <  1:117906/140‑1 (MQ=255)

                                                                               ttAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCa                                             >  1:40150/1‑140 (MQ=255)

                                                                                  aGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGt                                          <  1:481706/140‑1 (MQ=255)

                                                                                    aCTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTg                                        >  1:495355/1‑140 (MQ=255)

                                                                                       tcgtcgATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGccc                                     <  2:235133/140‑1 (MQ=255)

                                                                                        cgtcgATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCa                                    >  2:565591/1‑140 (MQ=255)

                                                                                        cgtcgATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCa                                    >  1:184834/1‑140 (MQ=255)

                                                                                        cgtcgATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGAGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCCGATCTGCGGCATGTTTcccc                                    >  2:415211/1‑139 (MQ=255)

                                                                                          tcgATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGa                                  <  2:436849/140‑1 (MQ=255)

                                                                                            gATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACg                                <  2:26592/140‑1 (MQ=255)

                                                                                              tttCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCa                              <  2:591013/140‑1 (MQ=255)

                                                                                               ttCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAg                             <  2:75217/140‑1 (MQ=255)

                                                                                                tctcAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGc                            <  2:442798/140‑1 (MQ=255)

                                                                                                  tcAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGccc                          >  1:475361/1‑140 (MQ=255)

                                                                                                          gcgcAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGCCCTTTGGTGa                  <  1:899645/140‑1 (MQ=255)

                                                                                                          gcgcAGCAACTGATTTCCACGCTCAACGGAAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGCCCTTTGGTGa                  >  1:9636/1‑140 (MQ=255)

                                                                                                           cgcAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGCCCTTTGGTGaa                 >  1:605282/1‑140 (MQ=255)

                                                                                                                          ccACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGCCCTTTGGTGAACGGCAACGGCGGCtt  >  2:392819/1‑140 (MQ=255)

TGTGCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCGGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGCCCTTTGGTGAACGGCAACGGCGGCTT  >  NC_000913/3590930‑3591191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A