Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	120	97.0	3206026	99.3%	3183583	140.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,770,354	T→C	intergenic (+168/‑18)	ydiK → / → rprA	putative transporter YdiK/small regulatory RNA RprA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,770,354	0	T	C	100.0%	157.8 / NA	42	intergenic (+168/‑18)	ydiK/rprA	putative transporter YdiK/small regulatory RNA RprA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/18); total (24/18)

ACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGTATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGATGGGCTTTTTTTTAACATTTTTCCGC  >  NC_000913/1770224‑1770490

accGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCctact                                                                                                                                 >  1:1137847/1‑140 (MQ=255)

 ccGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCctacta                                                                                                                                <  1:638745/140‑1 (MQ=255)

 ccGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCctacta                                                                                                                                >  1:616413/1‑140 (MQ=255)

  cGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATTCCTACTAt                                                                                                                               >  1:1187394/1‑140 (MQ=255)

  cGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTAt                                                                                                                               >  1:1583033/1‑140 (MQ=255)

    cTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTa                                                                                                                             >  2:463300/1‑140 (MQ=255)

    cTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTa                                                                                                                             >  2:934493/1‑140 (MQ=255)

       aTTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCt                                                                                                                          <  1:224970/140‑1 (MQ=255)

          gATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCAc                                                                                                                       <  2:632483/140‑1 (MQ=255)

                  cttgctCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATaa                                                                                                               <  1:123243/140‑1 (MQ=255)

                      ctctTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCa                                                                                                           >  2:407488/1‑140 (MQ=255)

                      ctctTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCa                                                                                                           >  1:903764/1‑140 (MQ=255)

                           ccccATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACata                                                                                                      >  2:1194575/1‑140 (MQ=255)

                                tatTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGAt                                                                                                 >  1:165761/1‑140 (MQ=255)

                                   tttCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTta                                                                                              >  2:791057/1‑140 (MQ=255)

                                    ttCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTtat                                                                                             <  1:1222705/140‑1 (MQ=255)

                                     tCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTtata                                                                                            >  2:660739/1‑140 (MQ=255)

                                     tCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTtata                                                                                            >  1:1356184/1‑140 (MQ=255)

                                            tACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATgg                                                                                     >  1:1539014/1‑140 (MQ=255)

                                            tACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATgg                                                                                     >  1:1260295/1‑140 (MQ=255)

                                                            gtagtaCCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGaa                                                                     >  1:521253/1‑140 (MQ=255)

                                                            gtagtaCCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGaa                                                                     >  1:987241/1‑140 (MQ=255)

                                                               gtaCCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAaaca                                                                  <  1:1375526/140‑1 (MQ=255)

                                                                      tCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAAtt                                                           <  2:1187394/140‑1 (MQ=255)

                                                                      tCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAAtt                                                           >  2:383687/1‑140 (MQ=255)

                                                                       cGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGTAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTg                                                          >  2:1313603/1‑140 (MQ=255)

                                                                       cGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTg                                                          <  2:903764/140‑1 (MQ=255)

                                                                        gCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGc                                                         >  2:371337/1‑140 (MQ=255)

                                                                               cTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTgtgtgt                                                  <  2:987241/140‑1 (MQ=255)

                                                                               cTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTgtgtgt                                                  <  1:934493/140‑1 (MQ=255)

                                                                               cTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTgtgtgt                                                  <  1:463300/140‑1 (MQ=255)

                                                                                        ttttAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGc                                         <  2:521253/140‑1 (MQ=255)

                                                                                        ttttAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGc                                         >  1:62371/1‑140 (MQ=255)

                                                                                        ttttAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGc                                         >  1:187347/1‑140 (MQ=255)

                                                                                         tttAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGcc                                        <  1:407488/140‑1 (MQ=255)

                                                                                         tttAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGcc                                        <  1:1321748/140‑1 (MQ=255)

                                                                                          ttAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGccc                                       <  2:391412/140‑1 (MQ=255)

                                                                                                      gaCGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGAt                           <  2:616413/140‑1 (MQ=255)

                                                                                                        cGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGATgg                         >  2:229849/1‑140 (MQ=255)

                                                                                                                     cGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGATGGGCTTTTTTTTAAc            >  1:477830/1‑140 (MQ=255)

                                                                                                                            aGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGATGGGCTTTTTTTTAACATTTTTc     <  2:187347/140‑1 (MQ=255)

                                                                                                                               ccGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGATGGGCTTTTTTTTAACATTTTTCCGc  <  2:1583033/140‑1 (MQ=255)

ACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGTATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGATGGGCTTTTTTTTAACATTTTTCCGC  >  NC_000913/1770224‑1770490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A