Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	120	97.0	3206026	99.3%	3183583	140.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,018,925	(C)_5→6	coding (540/1128 nt)	fliK →	flagellar hook‑length control protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,018,920	1	.	C	86.8%	219.8 / 26.8	68	coding (535/1128 nt)	fliK	flagellar hook‑length control protein
Reads supporting (aligned to +/- strand): ref base . (3/6); new base C (30/29); total (33/35)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CCTTTTTGCGATGTTGCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACG‑CCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGACACAGCCACTGC  >  NC_000913/2018784‑2019055

ccTTTTTGCGATGTTGCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACG‑ccc                                                                                                                                      >  1:431355/1‑140 (MQ=255)

ccTTTTTGCGATGTTGCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACG‑ccc                                                                                                                                      <  2:498887/140‑1 (MQ=255)

ccTTTTTGCGATGTTGCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACG‑ccc                                                                                                                                      >  1:1437045/1‑140 (MQ=255)

    tttGCGATGTTGCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACG‑CCCCCgg                                                                                                                                  <  2:152549/140‑1 (MQ=255)

     ttGCGATGTTGCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACG‑ccccccgg                                                                                                                                 <  1:1465776/140‑4 (MQ=255)

        cGATGTTGCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGTCCTCTGAGCAACTCGCAACAGCACAGCCTGATGACGCCCCCCGGcac                                                                                                                               <  2:1003510/140‑1 (MQ=255)

         gATGTTGCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGcaca                                                                                                                              <  1:28816/140‑1 (MQ=255)

          aTGTTGCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCacac                                                                                                                             >  2:295238/1‑140 (MQ=255)

              tGCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACG‑CCCCCGGCACACCAGCt                                                                                                                        <  1:479120/140‑1 (MQ=255)

               gCCGGGTTTTGACAATACGCCCAGAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCt                                                                                                                        <  2:1562868/140‑1 (MQ=255)

               gCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCt                                                                                                                        <  1:321157/140‑1 (MQ=255)

               gCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCt                                                                                                                        >  1:791567/1‑140 (MQ=255)

               gCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCt                                                                                                                        <  1:1041963/140‑1 (MQ=255)

               gCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCt                                                                                                                        >  1:1219384/1‑140 (MQ=255)

                ccGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTc                                                                                                                       >  2:1401065/1‑140 (MQ=255)

                     ttttGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCa                                                                                                                  >  2:404528/1‑140 (MQ=255)

                       ttGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCAtt                                                                                                                <  2:348502/140‑1 (MQ=255)

                           cAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAaca                                                                                                            <  1:110115/140‑1 (MQ=255)

                            aaTACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAacac                                                                                                           <  1:1356919/140‑1 (MQ=255)

                              tACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCg                                                                                                         >  2:441138/1‑140 (MQ=255)

                              tACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCg                                                                                                         <  2:986095/140‑1 (MQ=255)

                              tACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCg                                                                                                         >  1:410969/1‑140 (MQ=255)

                                  cccAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTgg                                                                                                     >  1:939671/1‑140 (MQ=255)

                                   ccAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGt                                                                                                    <  1:126510/140‑1 (MQ=255)

                                        gTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAg                                                                                               <  1:241499/140‑1 (MQ=255)

                                           aCTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAg                                                                                            >  1:1273959/1‑140 (MQ=255)

                                             tGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGcc                                                                                          <  1:233287/140‑1 (MQ=255)

                                             tGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGcc                                                                                          <  1:183089/140‑1 (MQ=255)

                                                 gcgcCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCaga                                                                                      >  2:1437999/1‑140 (MQ=255)

                                                       tCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAg                                                                                <  2:1219384/140‑1 (MQ=255)

                                                          aCCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGGCTTCTGAGCATCTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCgg                                                                             <  2:1264768/140‑1 (MQ=255)

                                                                 tACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGtcat                                                                      >  1:484960/1‑140 (MQ=255)

                                                                   ccGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACG‑CCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAg                                                                   <  1:382739/140‑1 (MQ=255)

                                                                   ccGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACG‑CCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAg                                                                   <  2:1593488/140‑1 (MQ=255)

                                                                      aCAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGca                                                                 <  2:1267803/140‑1 (MQ=255)

                                                                       cAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGcac                                                                <  1:890644/140‑1 (MQ=255)

                                                                       cAGAGAAACCAACGCTCATCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGcac                                                                <  2:692765/140‑1 (MQ=255)

                                                                         gagaAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCacac                                                              <  1:1546545/140‑1 (MQ=255)

                                                                          agaAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACAcc                                                             >  2:1030792/1‑140 (MQ=255)

                                                                          agaAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACAcc                                                             >  2:1056419/1‑140 (MQ=255)

                                                                                   cGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCGCCGGt                                                    >  1:552555/1‑140 (MQ=255)

                                                                                   cGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGt                                                    >  1:1256168/1‑140 (MQ=255)

                                                                                    gCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACG‑CCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGa                                                  <  1:15945/140‑1 (MQ=255)

                                                                                        ttCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCg                                               >  1:1520522/1‑140 (MQ=255)

                                                                                         tCACAAAGCCGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGc                                              <  2:1327462/140‑1 (MQ=255)

                                                                                         tCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGc                                              >  2:1108105/1‑140 (MQ=255)

                                                                                          cacaAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCt                                             >  2:257436/1‑140 (MQ=255)

                                                                                              aaaCTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTgccg                                         >  1:945373/1‑140 (MQ=255)

                                                                                               aaCTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTgccgc                                        <  1:295238/140‑1 (MQ=255)

                                                                                                   gACTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGc                                    <  2:1102071/140‑1 (MQ=255)

                                                                                                    aCTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGcc                                   >  2:1225243/1‑140 (MQ=255)

                                                                                                     cTTCTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGccc                                  >  1:975138/1‑140 (MQ=255)

                                                                                                        cTGAGCAACTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCt                               <  2:939671/140‑1 (MQ=255)

                                                                                                           aGCAACTCACAACAGCACAGCCCGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAAt                            <  2:484240/140‑1 (MQ=255)

                                                                                                                cTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTc                       <  1:1256856/140‑1 (MQ=255)

                                                                                                                cTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTc                       >  1:1101062/1‑140 (MQ=255)

                                                                                                                cTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTc                       >  1:300544/1‑140 (MQ=255)

                                                                                                                cTCACAACAGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAACAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTc                       >  2:773094/1‑140 (MQ=255)

                                                                                                                    caacaGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCcaca                   <  1:1521116/140‑1 (MQ=255)

                                                                                                                     aacaGCACAGCCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCacac                  <  1:1168587/140‑1 (MQ=255)

                                                                                                                       cagcacagcCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCa                <  2:431355/140‑1 (MQ=255)

                                                                                                                       cagcacagcCTGATGACG‑CCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAg               >  2:58290/1‑140 (MQ=255)

                                                                                                                          cacagcCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGac             >  2:1072495/1‑140 (MQ=255)

                                                                                                                          cacagcCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGac             >  1:1312145/1‑140 (MQ=255)

                                                                                                                          cacagcCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGac             >  1:885956/1‑140 (MQ=255)

                                                                                                                          cacagcCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGac             >  1:801804/1‑140 (MQ=255)

                                                                                                                          cacagcCTGATGACG‑CCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGaca            >  2:718499/1‑140 (MQ=255)

                                                                                                                          cacagcCTGATGACG‑CCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGaca            <  2:1521731/140‑1 (MQ=255)

                                                                                                                           acagcCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGaca            <  2:975138/140‑1 (MQ=255)

                                                                                                                             agcCTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGAcaca          >  1:688680/1‑140 (MQ=255)

                                                                                                                               ccTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGACACAGc        >  2:1468513/1‑140 (MQ=255)

                                                                                                                                cTGATGACGCCCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGACACAGcc       <  2:1437045/140‑1 (MQ=255)

                                                                                                                                    tgaCG‑CCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGACACAGCCACTGc  >  1:432583/1‑140 (MQ=255)

CCTTTTTGCGATGTTGCCGGGTTTTGACAATACGCCCAAAGTGACTGATGCGCCGTCAACCGTGTTACCGACAGAGAAACCAACGCTCTTCACAAAACTGACTTCTGAGCAACTCACAACAGCACAGCCTGATGACG‑CCCCCGGCACACCAGCTCAGCCATTAACACCGCTGGTAGCAGAAGCCCAGAGTAAAGCGGAAGTCATCAGCACACCTTCACCGGTGACCGCTGCCGCCAGCCCGCTAATCACTCCACACCAGACACAGCCACTGC  >  NC_000913/2018784‑2019055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A