Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	120	97.0	3206026	99.3%	3183583	140.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,984	(G)_7→8	coding (1041/1245 nt)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,977	1	.	G	97.7%	156.9 / ‑2.8	43	coding (1034/1245 nt)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (3/39); total (3/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

AATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGC‑GGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTG  >  NC_000913/3240838‑3241104

aaTATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGc                                                                                                                                   <  2:1498201/140‑1 (MQ=255)

       cgggcgCCGCAAACACCATTACCCTGTTGACGCTGGCTGCGGGTTATAAGGCGGGGATTTCCGGCGGTCTGCCCCCCGCGCCCCCCGTGGGGGGGGGGGGTTCTCTGTTTGCCCGTGGGGCCGCCGGCC‑Gggg‑ggggg‑gg                                                                                                                          >  2:597721/1‑140 (MQ=255)

       cgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGC‑ggggg‑gg                                                                                                                          <  1:1217883/140‑1 (MQ=255)

           cgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGC‑ggggg‑gggtct                                                                                                                      <  2:933410/140‑5 (MQ=255)

           cgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGC‑GGGGG‑GGtctc                                                                                                                      <  1:1589198/140‑1 (MQ=255)

              agcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTctg                                                                                                                    <  2:745904/140‑1 (MQ=255)

                  aTCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTG‑TGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTctgctgct                                                                                                               <  2:665206/140‑1 (MQ=255)

                   tCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTctgctgct                                                                                                               <  2:1429354/140‑1 (MQ=255)

                   tCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTctgctgct                                                                                                               <  2:91165/140‑1 (MQ=255)

                          tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCa                                                                                                        <  2:228525/140‑1 (MQ=255)

                          tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCa                                                                                                        >  2:861151/1‑140 (MQ=255)

                          tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCa                                                                                                        <  1:1309723/140‑1 (MQ=255)

                          tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGGTcccc                                                                                                        >  2:1140919/1‑139 (MQ=255)

                            ccGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACt                                                                                                      >  1:1099768/1‑140 (MQ=255)

                            ccGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATcccccc                                                                                                      >  2:1026980/1‑137 (MQ=255)

                             cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGGTCTGCTGCTGGTccccccg                                                                                                     >  2:602352/1‑136 (MQ=255)

                              gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTgg                                                                                                    <  1:1384707/140‑1 (MQ=255)

                               tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGc                                                                                                   <  1:1516232/140‑1 (MQ=255)

                               tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGc                                                                                                   <  1:1140708/140‑1 (MQ=255)

                               tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGc                                                                                                   >  1:965192/1‑140 (MQ=255)

                               tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGc                                                                                                   <  1:94821/140‑1 (MQ=255)

                               tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGc                                                                                                   >  2:736599/1‑140 (MQ=255)

                               tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCACCGGc                                                                                                   >  2:1376660/1‑140 (MQ=255)

                               tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCCCTGCTGCTGGTCCCCCTGGc                                                                                                   >  2:630247/1‑140 (MQ=255)

                               tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTACCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGc                                                                                                   <  1:1515698/140‑1 (MQ=255)

                                gtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGcc                                                                                                  <  1:1108042/140‑1 (MQ=255)

                                 ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGGTCTGGTGCTGATCCCCCTCGccc                                                                                                 >  2:1469335/1‑139 (MQ=255)

                                 ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCCCCGGccc                                                                                                 >  2:1418046/1‑139 (MQ=255)

                                 ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCACTGGccc                                                                                                 >  1:1122481/1‑139 (MQ=255)

                                 ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCACCGGccc                                                                                                 >  1:342988/1‑139 (MQ=255)

                                   gACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGt                                                                                               >  1:871592/1‑140 (MQ=255)

                                    aCGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                                              <  2:44704/140‑1 (MQ=255)

                                    aCGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCCCCGGCCCGTa                                                                                              >  2:1296168/1‑140 (MQ=255)

                                        tggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCCCTGGCCTGGAatat                                                                                          >  1:950/1‑140 (MQ=255)

                                         ggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCCGATCCCACCGGCCCGTAATATg                                                                                         >  2:1256186/1‑140 (MQ=255)

                                          gctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGc                                                                                                   <  2:1217594/129‑1 (MQ=255)

                                          gctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCACTGGc                                                                                                   >  1:1217594/1‑129 (MQ=255)

                                           ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCCCCGGGCTGTAATATGtt                                                                                       >  2:490636/1‑140 (MQ=255)

                                             gCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCg                                                                                     <  1:292084/140‑1 (MQ=255)

                                                     tACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCg                                                                             <  1:602352/140‑1 (MQ=255)

                                                       cGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                           <  2:1117535/140‑1 (MQ=255)

                                                           gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCCCTGCTTCTGATCCCCCCCGCCTGTTATATGGTTGGTGTTTTGAACGat                                                                       >  2:1235150/1‑140 (MQ=255)

                                                           gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTATGGCGCATCCGGCG‑TGGCGGGGGG‑GGGGTCTCCTGCTGACCCCCCCGGCCCGGAATAAGTTCGGGATTTCGAAAGaa                                                                       >  2:537690/1‑139 (MQ=255)

                                                             gTATTCCGGTCGATCTGCCCGCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGatat                                                                     <  2:1305073/140‑1 (MQ=255)

                                                             gTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGatat                                                                     <  1:899221/140‑1 (MQ=255)

                                                              tATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATc                                                                    <  1:499644/140‑1 (MQ=255)

                                                               aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCg                                                                   <  1:1463779/140‑1 (MQ=255)

                                                                ttCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGc                                                                  <  2:1291307/140‑1 (MQ=255)

                                                                 tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCCCCGGCCCGTAATATGTTCGGTGTTTCGGACGAGATCGcc                                                                 >  2:577311/1‑140 (MQ=255)

                                                                 tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGAGCCCCCTGGCCTGTGATATGTTCGGGGTTTCGGACGACATCGcc                                                                 >  2:1161134/1‑140 (MQ=255)

                                                                    ggTCGATCTGCCCTGGGCGCTGCTGTTGAGCATGGTGGCTTCTCTGTGTGCCTGTGGCGCATGCGGCG‑TGGC‑GGGGGTGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATg                                                              <  2:1459599/140‑1 (MQ=255)

                                                                     gTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGc                                                             <  2:14497/140‑1 (MQ=255)

                                                                      tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCCCTGCTGCTGATCCCACCCGCCCGGAATATGTTCGGGGTTTCGAACGATGTCGCCATGCa                                                            >  2:956735/1‑140 (MQ=255)

                                                                                 aCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCg                                                 <  1:1256186/140‑1 (MQ=255)

                                                                                  cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCCCTGGCCCGTAATATGTTCGGGATTTCGAACGATATCGCCATGCAGGGGGGTGCCgc                                                >  2:944692/1‑139 (MQ=255)

                                                                                        tgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGGTCCCCCCGGCCCGTAATATGTTCGGTATTTCGAACGATATCGCCCTGCAGGGGGGTGCCGTCGGCtt                                          >  2:946587/1‑140 (MQ=255)

                                                                                              tGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat                                    <  2:378357/140‑1 (MQ=255)

                                                                                              tGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat                                    <  2:1256561/140‑1 (MQ=255)

                                                                                              tGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat                                    <  1:1568053/140‑1 (MQ=255)

                                                                                               gAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCACTGGCCCGTAATATGTTCGGGATTTTGAACGATATCGCCCTGCAGGGGGGTGCCGTCGGCTTTttcatc                                   >  1:150716/1‑140 (MQ=255)

                                                                                                  cGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGGATTTCGAACGATATCGCCCTGCAGGGGGGTGCCGGCGGCTTTTTCATCGGc                                >  1:363600/1‑140 (MQ=255)

                                                                                                   gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                               <  1:1351445/140‑1 (MQ=255)

                                                                                                   gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                               <  1:144381/140‑1 (MQ=255)

                                                                                                   gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                               <  2:965192/140‑1 (MQ=255)

                                                                                                   gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                               <  1:1019870/140‑1 (MQ=255)

                                                                                                   gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                               <  2:110079/140‑1 (MQ=255)

                                                                                                      gtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTAt                            <  1:861151/140‑1 (MQ=255)

                                                                                                          cTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCa                        <  1:360078/140‑1 (MQ=255)

                                                                                                             ctctGTGTGCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGa                     <  1:1296168/140‑1 (MQ=255)

                                                                                                                   gtgCCTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCACTGGGCTGTAATATGTTCGGGATTTCGAACGATATCGCCATGCGGGTGGTTGCCGTCGGCCTTATCATCGGCGGATTGCAGGGCTCTTg               >  2:699068/1‑140 (MQ=255)

                                                                                                                      ccTGTGGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCCCTGGCCTGTAATATGTTCGGGATTTCGAACGATATCGCCATGCAGGGGGGTGCCGTCGGCCTTGTCATCGGCGGATTGCAGGAGGCTTGCgg            >  2:894048/1‑139 (MQ=255)

                                                                                                                        tgtgGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGaaa          <  1:262550/140‑1 (MQ=255)

                                                                                                                        tgtgGCGCATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGaaa          <  2:871592/140‑1 (MQ=255)

                                                                                                                        tgtgGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCCCCGGCCCGTAAAATGTTCGGGATTTCGAACGGTATCGCCATGCAGGGGGGTGCCGTCGGCCTTATCATCGGCGTATTGCAGGACTCTTTCGaaa          >  2:872282/1‑140 (MQ=255)

                                                                                                                          tgGCGCATCCGGCG‑TGGCGGGGGG‑GGGCTCTGCTGCTGATCCCCCTGGCCTGTAATATTTTCGGGATTTTGAACGATATCGCCATGCAGGGGGGTGCCGGCGGCTTTTTCATCGGCGGATTGCAGGGCTCTTGCGAAAcc        >  2:630180/1‑140 (MQ=255)

                                                                                                                             cgcATCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACcgcg     <  2:1099768/140‑1 (MQ=255)

                                                                                                                                aTCCGGCGTTGCC‑GGGAG‑TGTCTATGCTACTGATCCCACTGGCCTGTACTATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTGTCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTg  <  2:880767/140‑1 (MQ=255)

                                                                                                                                aTCCGGCG‑TGGCGGGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTg  <  1:854292/140‑1 (MQ=255)

AATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCG‑TGGC‑GGGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTG  >  NC_000913/3240838‑3241104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A