Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	120	97.0	3206026	99.3%	3183583	140.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,551,302	(T)_5→6	coding (1172/2394 nt)	malP ←	maltodextrin phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,551,297	1	.	T	98.8%	284.5 / ‑3.5	82	coding (1177/2394 nt)	malP	maltodextrin phosphorylase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (37/44); total (38/44)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACAC‑TT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCAT  >  NC_000913/3551160‑3551429

gTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACAC‑tt                                                                                                                                      >  2:650786/1‑140 (MQ=255)

 tGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACAC‑tt‑t                                                                                                                                    >  2:531408/1‑140 (MQ=255)

     atTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACAC‑tt‑ttttc                                                                                                                                >  1:1002318/1‑138 (MQ=255)

       tCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACAC‑tt‑ttttcat                                                                                                                              >  1:638290/1‑136 (MQ=255)

       tCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACAC‑TTCTTT‑CAt                                                                                                                              >  1:1591698/1‑140 (MQ=255)

           ggAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGcc                                                                                                                          >  2:995044/1‑140 (MQ=255)

               cAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCATACTTT‑TTT‑CATCGCCCGGc                                                                                                                      <  2:1110794/140‑1 (MQ=255)

               cAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGc                                                                                                                      <  1:1181814/140‑1 (MQ=255)

               cAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGc                                                                                                                      <  2:50362/140‑1 (MQ=255)

               cAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGc                                                                                                                      <  1:132208/140‑1 (MQ=255)

               cAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGc                                                                                                                      >  2:1121650/1‑140 (MQ=255)

                  aTCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAg                                                                                                                   <  1:67291/140‑1 (MQ=255)

                  aTCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAg                                                                                                                   >  1:773613/1‑140 (MQ=255)

                       tccccaccaGATCCGAGTGCAGCGCCGCAACACCGTTCCCCGCGAACCCGCCAACCACACACAGGTTCGCCATATTCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGtttt                                                                                                              <  2:328569/137‑1 (MQ=255)

                       tCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGtttt                                                                                                              >  1:1249517/1‑140 (MQ=255)

                       tCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGtttt                                                                                                              >  2:867519/1‑140 (MQ=255)

                       tCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGtttt                                                                                                              >  2:788493/1‑140 (MQ=255)

                        caccacCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTtc                                                                                                             <  2:412807/140‑1 (MQ=255)

                          ccaccaGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTtctc                                                                                                           <  1:436561/140‑1 (MQ=255)

                             ccaGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCACCCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTAc                                                                                                        <  2:325059/140‑1 (MQ=255)

                             ccaGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTAc                                                                                                        <  2:1458410/140‑1 (MQ=255)

                              caGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTAcc                                                                                                       >  1:1306996/1‑140 (MQ=255)

                               aGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCa                                                                                                      >  1:879720/1‑140 (MQ=255)

                                gATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAg                                                                                                     <  2:430004/140‑1 (MQ=255)

                                 aTCCGAGTGCAGCGCCGCAACACGTTTCCCCGGGAAGCCGCCAGCCACACACAGGCTCGCCATACGCTCTTGTTTGTGGTGCACCACCGCCAGTTTGGCCCGCACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGc                                                                                                    <  2:1057537/140‑1 (MQ=255)

                                 aTCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGc                                                                                                    >  2:724108/1‑140 (MQ=255)

                                 aTCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGc                                                                                                    >  2:1039824/1‑140 (MQ=255)

                                   ccGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGt                                                                                                  >  1:1403322/1‑140 (MQ=255)

                                   ccGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGt                                                                                                  >  1:526134/1‑140 (MQ=255)

                                        tGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTaa                                                                                             >  1:1250796/1‑140 (MQ=255)

                                           ggcgcCGCAACCCCGTTCCCCGCGAACCCGCCAACCACCCACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGc                                                                                          <  2:81487/139‑1 (MQ=255)

                                           aGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGc                                                                                          >  2:840564/1‑140 (MQ=255)

                                             cgccgcAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGa                                                                                        >  1:63616/1‑140 (MQ=255)

                                             cgccgcAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGa                                                                                        >  1:1112630/1‑140 (MQ=255)

                                             cgccgcAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGa                                                                                        >  1:561888/1‑140 (MQ=255)

                                             cgccgcAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGa                                                                                        >  2:1440503/1‑140 (MQ=255)

                                                     ccccGTCCCCCGCGAACCCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAAt                                                                                <  2:1585855/138‑1 (MQ=255)

                                                     ccccGTCCCCCGCGAACCCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAAt                                                                                <  2:1571252/138‑1 (MQ=255)

                                                       ccttcccccGCGAAACCGCCACCCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCCCCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAAttt                                                                              <  2:1591698/133‑1 (MQ=255)

                                                       ccGTTCACCGCGAACCCGCCAACCACACACAGGTCCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAAttt                                                                              <  2:773613/140‑1 (MQ=255)

                                                       ccGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAAttt                                                                              <  1:531408/140‑1 (MQ=255)

                                                          ttCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGt                                                                           >  1:1029305/1‑140 (MQ=255)

                                                          ttCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGt                                                                           >  2:374757/1‑140 (MQ=255)

                                                            cACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTta                                                                         <  1:995044/140‑1 (MQ=255)

                                                            cACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTta                                                                         <  1:370814/140‑1 (MQ=255)

                                                             aCCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTtaa                                                                        <  1:650786/140‑1 (MQ=255)

                                                              ccGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTtaat                                                                       <  2:1447480/140‑1 (MQ=255)

                                                                        gCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCAtg                                                             <  2:561888/140‑1 (MQ=255)

                                                                         ccAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCAtgt                                                            >  1:1263858/1‑140 (MQ=255)

                                                                         ccAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCAtgt                                                            <  1:1162779/140‑1 (MQ=255)

                                                                         ccAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCAtgt                                                            >  2:1326712/1‑140 (MQ=255)

                                                                             ccACACTCAGGTTCGCCATATGCACTTGTTTGTCGTGCGCCACCGCCAGTTTGGCCCACAC‑TT‑TTTGCATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGgcg                                                        <  2:1265396/140‑1 (MQ=255)

                                                                             ccACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGgcg                                                        <  1:636413/140‑1 (MQ=255)

                                                                             ccACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGgcg                                                        <  1:1039824/140‑1 (MQ=255)

                                                                             ccACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGgcg                                                        <  2:1249517/140‑1 (MQ=255)

                                                                              cacacacaGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGgcgc                                                       <  2:1002318/140‑1 (MQ=255)

                                                                                     aGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTa                                                <  1:788493/140‑1 (MQ=255)

                                                                                       gTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAg                                              >  2:1363182/1‑140 (MQ=255)

                                                                                       gTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAg                                              <  1:1121650/140‑1 (MQ=255)

                                                                                       gTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAg                                              >  2:1317966/1‑140 (MQ=255)

                                                                                       gTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAg                                              >  2:1316105/1‑140 (MQ=255)

                                                                                          cGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCt                                           >  1:546579/1‑140 (MQ=255)

                                                                                          cGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCt                                           <  2:74258/140‑1 (MQ=255)

                                                                                               tatGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCAc                                      >  2:1322963/1‑140 (MQ=255)

                                                                                                 tGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCa                                    >  2:53138/1‑140 (MQ=255)

                                                                                                 tGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCa                                    >  2:1027965/1‑140 (MQ=255)

                                                                                                 tGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCa                                    >  1:660630/1‑140 (MQ=255)

                                                                                                       tgtttgtCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTca                              >  2:726900/1‑140 (MQ=255)

                                                                                                       tgtttgtCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTca                              >  2:1366358/1‑140 (MQ=255)

                                                                                                       tgttggtCGTGCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTca                              <  2:1029305/140‑1 (MQ=255)

                                                                                                                 gCACCACCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCg                    <  1:374757/140‑1 (MQ=255)

                                                                                                                  caccacCGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGt                   >  1:1354715/1‑140 (MQ=255)

                                                                                                                       ccGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAg              <  1:867519/140‑1 (MQ=255)

                                                                                                                       ccGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAg              <  2:383108/140‑1 (MQ=255)

                                                                                                                       ccGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAg              <  1:1440503/140‑1 (MQ=255)

                                                                                                                       ccGCCAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGGTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAg              >  1:491870/1‑140 (MQ=255)

                                                                                                                       ccGCCACTTTGGCCCAGACTTT‑TTT‑CATCGCCCGCCCAGGCTTTCTCTACCAGCGTTTTAAAGCGAGGATTAATTTCGGTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTCGCACATGCCAGCGTTCCAg              >  2:1492307/1‑140 (MQ=255)

                                                                                                                          ccAGTTTGGCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAgcgc           >  2:467825/1‑140 (MQ=255)

                                                                                                                                  gCCCACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCa   >  2:1032605/1‑140 (MQ=255)

                                                                                                                                   cccACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCAt  <  1:785587/140‑1 (MQ=255)

                                                                                                                                   cccACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCAt  <  2:546579/140‑1 (MQ=255)

                                                                                                                                   cccACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCAt  <  1:1326712/140‑1 (MQ=255)

                                                                                                                                   cccACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCAt  <  2:135694/140‑1 (MQ=255)

                                                                                                                                   cccACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCAt  <  2:1234319/140‑1 (MQ=255)

                                                                                                                                   cccACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCAt  <  1:604349/140‑1 (MQ=255)

                                                                                                                                   cccACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCAt  <  1:413326/140‑1 (MQ=255)

                                                                                                                                   cccACACTTT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCAt  <  2:879720/140‑1 (MQ=255)

GTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACAC‑TT‑TTT‑CATCGCCCGGCCAGGTTTTCTCTACCAGCGTTTTAAAGCGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCAT  >  NC_000913/3551160‑3551429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A