Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	713,628	C→T	A24V (GCG→GTG)	pgm →	phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	713,628	0	C	T	100.0%	166.2 / NA	48	A24V (GCG→GTG)	pgm	phosphoglucomutase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/20); total (28/20)

TCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAG  >  NC_000913/713492‑713755

tCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCa                                                                                                                              <  1:814769/140‑1 (MQ=255)

tCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCa                                                                                                                              <  2:698228/140‑1 (MQ=255)

   cggcggAATTGATTGAGAAGGTTTGCGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAata                                                                                                                           <  1:707157/140‑1 (MQ=255)

     gcggAATTGATTGAGAAGGTTTGCGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAAtatt                                                                                                                         <  1:1064407/140‑1 (MQ=255)

                            cGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAggg                                                                                                  >  1:986878/1‑140 (MQ=255)

                            cGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAggg                                                                                                  >  2:449495/1‑140 (MQ=255)

                            cGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAggg                                                                                                  <  2:875000/140‑1 (MQ=255)

                                aCTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATg                                                                                              <  2:871107/140‑1 (MQ=255)

                                             ttGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGt                                                                                 >  2:380430/1‑140 (MQ=255)

                                                     aaaggacaaagCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCg                                                                         >  1:30566/1‑140 (MQ=255)

                                                        ggacaaagCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTa                                                                      <  2:586684/140‑1 (MQ=255)

                                                             aagCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACt                                                                    >  2:1024047/1‑137 (MQ=255)

                                                             aagCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACt                                                                    <  1:1024047/137‑1 (MQ=255)

                                                              agCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCg                                                                >  2:429568/1‑140 (MQ=255)

                                                              agCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCg                                                                <  1:380430/140‑1 (MQ=255)

                                                                cAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGt                                                              <  2:30566/140‑1 (MQ=255)

                                                                   tGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCAc                                                           >  2:482468/1‑140 (MQ=255)

                                                                   tGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCAc                                                           >  2:53259/1‑140 (MQ=255)

                                                                   tGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCAc                                                           <  1:630004/140‑1 (MQ=255)

                                                                      cAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGt                                                        >  1:1076473/1‑140 (MQ=255)

                                                                        aTCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCgg                                                                        <  1:270754/122‑1 (MQ=255)

                                                                        aTCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCgg                                                                        >  2:270754/1‑122 (MQ=255)

                                                                        aTCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTgg                                                      >  1:126665/1‑140 (MQ=255)

                                                                                   tGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAGATTCGGTACTTCCGGTCACCGTGGCAGTGCAgcgc                                           >  2:747980/1‑140 (MQ=255)

                                                                                   tGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAgcgc                                           >  2:497474/1‑140 (MQ=255)

                                                                                   tGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAgcgc                                           >  1:1014600/1‑140 (MQ=255)

                                                                                       ggCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCca                                       <  1:429568/140‑1 (MQ=255)

                                                                                        gCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCcac                                      >  2:728446/1‑140 (MQ=255)

                                                                                          cAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAg                                    >  2:772962/1‑140 (MQ=255)

                                                                                                      caGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGcc                        >  2:1004108/1‑140 (MQ=255)

                                                                                                       agagTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCg                       >  2:157321/1‑140 (MQ=255)

                                                                                                        gagTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGc                      <  1:1004108/140‑1 (MQ=255)

                                                                                                         agTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGca                     >  2:66474/1‑140 (MQ=255)

                                                                                                         agTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGca                     >  2:937853/1‑140 (MQ=255)

                                                                                                         agTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGca                     >  1:232920/1‑140 (MQ=255)

                                                                                                          gTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGcgc                    <  1:449495/140‑3 (MQ=255)

                                                                                                           tgatttgattAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGcaca                   >  2:1039156/1‑140 (MQ=255)

                                                                                                                tgattAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTg              >  2:90906/1‑140 (MQ=255)

                                                                                                                  attAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGc            >  1:169685/1‑140 (MQ=255)

                                                                                                                   ttAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCg           <  2:460452/140‑1 (MQ=255)

                                                                                                                     aaCGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGAt         >  2:474966/1‑140 (MQ=255)

                                                                                                                       cGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCg       >  2:412253/1‑140 (MQ=255)

                                                                                                                         tCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCt     <  2:232920/140‑1 (MQ=255)

                                                                                                                         tCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCt     <  2:126665/140‑1 (MQ=255)

                                                                                                                         tCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCt     <  1:482468/140‑1 (MQ=255)

                                                                                                                            cccAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGc      >  2:322967/1‑136 (MQ=255)

                                                                                                                            cccAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGc      <  1:322967/136‑1 (MQ=255)

                                                                                                                            cccAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAg  >  2:963108/1‑140 (MQ=255)

TCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAG  >  NC_000913/713492‑713755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A