Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,162,938	A→G	S101G (AGC→GGC)	lpoB →	outer membrane lipoprotein ‑ activator of MrcB activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,162,938	0	A	G	100.0%	109.7 / NA	33	S101G (AGC→GGC)	lpoB	outer membrane lipoprotein ‑ activator of MrcB activity
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/17); total (16/17)

CCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTAGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACTGTCGATGGC  >  NC_000913/1162802‑1163074

cccGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCg                                                                                                                                       <  1:361279/140‑1 (MQ=255)

       cagcCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCctgct                                                                                                                                >  2:593729/1‑140 (MQ=255)

       cagcCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCctgct                                                                                                                                >  1:113894/1‑140 (MQ=255)

          ccAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGt                                                                                                                             <  1:504798/140‑1 (MQ=255)

            aGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTg                                                                                                                           <  1:969418/140‑1 (MQ=255)

            aGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTg                                                                                                                           <  1:573893/140‑1 (MQ=255)

                      gAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTaa                                                                                                                 <  1:780830/140‑1 (MQ=255)

                       aGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTaac                                                                                                                <  1:226596/140‑1 (MQ=255)

                       aGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTaac                                                                                                                <  2:113894/140‑1 (MQ=255)

                       aGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTaac                                                                                                                >  2:620155/1‑140 (MQ=255)

                       aGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTaac                                                                                                                <  1:50309/140‑1 (MQ=255)

                                      cTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGt                                                                                                 >  1:135331/1‑140 (MQ=255)

                                             gCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGa                                                                                          >  2:604958/1‑140 (MQ=255)

                                              ccTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGaa                                                                                         <  1:620155/140‑1 (MQ=255)

                                                            tCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGa                                                                           >  1:699288/1‑140 (MQ=255)

                                                                 aTTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGaa                                                                      >  2:921917/1‑140 (MQ=255)

                                                                      gACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCt                                                                 <  1:799665/140‑1 (MQ=255)

                                                                          ggAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGa                                                             >  1:493099/1‑140 (MQ=255)

                                                                                gcgcAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATgcg                                                       <  1:926624/140‑1 (MQ=255)

                                                                                gcgcAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATgcg                                                       <  1:279469/140‑1 (MQ=255)

                                                                                   cAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTg                                                    >  2:795399/1‑140 (MQ=255)

                                                                                      tGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCt                                                 <  1:501671/140‑1 (MQ=255)

                                                                                              aTGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACgg                                         >  2:606855/1‑140 (MQ=255)

                                                                                                             cTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGt                          >  2:11010/1‑140 (MQ=255)

                                                                                                             cTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGt                          <  2:135331/140‑1 (MQ=255)

                                                                                                                ggggCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTc                       >  2:939631/1‑140 (MQ=255)

                                                                                                                         ggggTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCa              <  1:604958/140‑1 (MQ=255)

                                                                                                                         ggggTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCa              <  1:593729/140‑1 (MQ=255)

                                                                                                                            gTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACt           >  2:588379/1‑140 (MQ=255)

                                                                                                                                 tGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACTGTCGa      >  2:3685/1‑140 (MQ=255)

                                                                                                                                 tGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACTGTCGa      >  1:840209/1‑140 (MQ=255)

                                                                                                                                    gggTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACTGTCGATgg   <  2:840209/140‑1 (MQ=255)

                                                                                                                                     ggTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACTGTCGATGGc  >  2:1045073/1‑140 (MQ=255)

CCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTAGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACTGTCGATGGC  >  NC_000913/1162802‑1163074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A