Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,177,836	T→C	S173P (TCG→CCG)	lolE →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,177,836	0	T	C	100.0%	121.0 / NA	35	S173P (TCG→CCG)	lolE	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/18); total (17/18)

CGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACTCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCCAACAATATCTTGATAT  >  NC_000913/1177700‑1177969

cgcATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGa                                                                                                                                    <  1:186706/140‑1 (MQ=255)

   aTTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATc                                                                                                                                 >  2:905981/1‑140 (MQ=255)

    ttACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATcc                                                                                                                                <  1:458514/140‑1 (MQ=255)

     tACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCTGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATccc                                                                                                                               >  1:830087/1‑140 (MQ=255)

     tACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAACGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATccc                                                                                                                               <  2:316262/140‑1 (MQ=255)

     tACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATccc                                                                                                                               >  1:230539/1‑140 (MQ=255)

        ccTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAg                                                                                                                            >  2:597431/1‑140 (MQ=255)

            gTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATa                                                                                                                        >  1:233195/1‑140 (MQ=255)

                 tCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATGCCGAGCATAAACTg                                                                                                                   >  2:1078755/1‑140 (MQ=255)

                 tCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGc                                                                                                                           >  1:1073376/1‑132 (MQ=255)

                 tCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGc                                                                                                                           <  2:1073376/132‑1 (MQ=255)

                 tCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGGGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTg                                                                                                                   >  1:985147/1‑140 (MQ=255)

                 tCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGATGTGAAAGAGAAGCATTGGGGTTGGGGGTCGAGTATGATCCCCAACCCGAATCCCGAGCATGAAcgg                                                                                                                   >  2:60499/1‑138 (MQ=255)

                    aGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCTGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATg                                                                                                                <  2:830087/140‑1 (MQ=255)

                                  cgcAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACgtgt                                                                                                  >  2:1007022/1‑140 (MQ=255)

                                       ttttAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGtt                                                                                             <  1:828944/140‑1 (MQ=255)

                                       ttttAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGtt                                                                                             <  1:685624/140‑1 (MQ=255)

                                           aaaGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCa                                                                                         <  2:230539/140‑1 (MQ=255)

                                                 ggCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGc                                                                                   <  2:985147/140‑1 (MQ=255)

                                                 ggCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGc                                                                                   <  1:807454/140‑1 (MQ=255)

                                                                  catcGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTTTGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGa                                                                  <  2:951243/140‑1 (MQ=255)

                                                                  catcGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGa                                                                  <  2:134328/140‑1 (MQ=255)

                                                                   atcGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAg                                                                 <  1:1007022/140‑1 (MQ=255)

                                                                    tcGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTTTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGt                                                                >  2:748093/1‑140 (MQ=255)

                                                                                tGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGAt                                                    <  1:597431/140‑1 (MQ=255)

                                                                                                    aaGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATccc                                >  1:976351/1‑140 (MQ=255)

                                                                                                     aGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCg                               >  1:256655/1‑140 (MQ=255)

                                                                                                          ggggATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGc                          >  1:985085/1‑140 (MQ=255)

                                                                                                           gggATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCg                         >  1:541996/1‑140 (MQ=255)

                                                                                                            ggATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCgg                        <  2:256655/140‑1 (MQ=255)

                                                                                                                  ggTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCTATGATCCCGCTGGCGGATGccc                  <  2:976351/140‑1 (MQ=255)

                                                                                                                       cGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCcaacaa             >  1:490126/1‑140 (MQ=255)

                                                                                                                                tCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCCAACAATATCTTGat    <  1:1078755/140‑1 (MQ=255)

                                                                                                                                  cccAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCCAACAATATCTTGatat  <  2:490126/140‑1 (MQ=255)

                                                                                                                                  cccAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCCAACAATATCTTGatat  >  2:631904/1‑140 (MQ=255)

                                                                                                                                  cccAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTAGTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCCAACAATATCTTGatat  >  2:1009471/1‑140 (MQ=255)

CGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACTCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCCAACAATATCTTGATAT  >  NC_000913/1177700‑1177969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A