Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,227,995	C→T	D105D (GAC→GAT)	ycgL →	PF05166 family protein YcgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,227,995	0	C	T	100.0%	98.8 / NA	29	D105D (GAC→GAT)	ycgL	PF05166 family protein YcgL
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/15); total (14/15)

GAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGCAAGGTGCGCTGCTGGATTATCCGGTGAG  >  NC_000913/1227860‑1228128

gaagaaACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTa                                                                                                                                   <  1:811854/140‑1 (MQ=255)

  agaaACTTGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAAc                                                                                                                                 >  2:524255/1‑140 (MQ=255)

               tGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGatat                                                                                                                    >  1:691378/1‑140 (MQ=255)

               tGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGatat                                                                                                                    >  1:725730/1‑140 (MQ=255)

               tGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGatat                                                                                                                    >  1:844073/1‑140 (MQ=255)

                           aaaGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCa                                                                                                        <  2:648476/140‑1 (MQ=255)

                                         cATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGgc                                                                                          >  1:745233/1‑140 (MQ=255)

                                         cATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGgc                                                                                          >  2:454859/1‑140 (MQ=255)

                                                  aGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGtttt                                                                                 >  1:504540/1‑140 (MQ=255)

                                                  aGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGtttt                                                                                 >  1:105368/1‑140 (MQ=255)

                                                    cAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCa                                                                               <  2:691378/140‑1 (MQ=255)

                                                       ggTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCatat                                                                            <  1:196821/140‑1 (MQ=255)

                                                         ttACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATcc                                                                          <  2:380300/140‑1 (MQ=255)

                                                          tACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCa                                                                         >  1:504809/1‑140 (MQ=255)

                                                             tATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTc                                                                                >  2:86637/1‑130 (MQ=255)

                                                             tATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTc                                                                                <  1:86637/130‑1 (MQ=255)

                                                                 tGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAg                                                                  >  2:336091/1‑140 (MQ=255)

                                                                           gCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGaa                                                        <  2:504809/140‑1 (MQ=255)

                                                                             caccacCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACg                                                      <  2:906759/140‑1 (MQ=255)

                                                                              accaccCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGt                                                     <  2:844073/140‑1 (MQ=255)

                                                                                           ttGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACAt                                        >  2:685042/1‑140 (MQ=255)

                                                                                                     aaCATCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGc                              <  2:725730/140‑1 (MQ=255)

                                                                                                       aaTCTTTCCGTCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGCaa                            <  2:363498/139‑1 (MQ=255)

                                                                                                                 tCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGCAAGGTg                        <  1:442735/134‑1 (MQ=255)

                                                                                                                 tCATGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGCAAGGTg                        >  2:442735/1‑134 (MQ=255)

                                                                                                                   aTGGGGCAGAAAACAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGCAAGGTGCGctgctg                >  2:824462/1‑140 (MQ=255)

                                                                                                                            aaaaCAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGCAAGGTGCGCTGCTGGATTATCCg       <  2:542751/140‑1 (MQ=255)

                                                                                                                            aaaaCAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGCAAGGTGCGCTGCTGGATTATCCg       <  2:46174/140‑1 (MQ=255)

                                                                                                                              aaCAGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGCAAGGTGCGCTGCTGGATTATCCGGt     <  1:824462/140‑1 (MQ=255)

                                                                                                                                 aGACGATACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGCAAGGTGCGCTGCTGGATTATCCGGTGAg  <  2:105368/140‑1 (MQ=255)

GAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGCAAGGTGCGCTGCTGGATTATCCGGTGAG  >  NC_000913/1227860‑1228128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A