Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	116	98.7	3298914	99.2%	3272522	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,236,278	(G)_6→7	coding (964/1533 nt)	ycgB ←	PF04293 family protein YcgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,236,272	1	.	G	98.8%	311.7 / ‑3.4	83	coding (970/1533 nt)	ycgB	PF04293 family protein YcgB
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (32/50); total (32/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCCAGTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑GGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTTCATCACCT  >  NC_000913/1236135‑1236406

cagccagTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTCCCACGGGCTGTTATA‑gg                                                                                                                                       <  1:573344/140‑1 (MQ=255)

       tCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑ggg‑ggggct                                                                                                                               >  1:1455210/1‑137 (MQ=255)

       tCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑ggg‑ggggct                                                                                                                               >  1:511931/1‑137 (MQ=255)

       tCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑ggg‑ggggct                                                                                                                               >  1:878381/1‑137 (MQ=255)

       tCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑ggg‑ggggct                                                                                                                               >  2:1065443/1‑137 (MQ=255)

       tCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑ggg‑ggggct                                                                                                                               >  1:1203495/1‑137 (MQ=255)

        cGGAAGCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑ggg‑ggggctg                                                                                                                              <  1:891821/140‑5 (MQ=255)

        cGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑ggg‑ggggctt                                                                                                                              <  1:1331698/140‑5 (MQ=255)

         ggAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑ggg‑ggggctgg                                                                                                                             <  2:1013193/140‑6 (MQ=255)

             ccGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGa                                                                                                                          <  2:833854/140‑1 (MQ=255)

             ccGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGa                                                                                                                          <  2:447315/140‑1 (MQ=255)

             ccGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGa                                                                                                                          <  1:98281/140‑1 (MQ=255)

             ccGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGa                                                                                                                          <  1:1345787/140‑1 (MQ=255)

             ccGGCGATATCCGGGAACCAGTATTTGTCTTCTCCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGa                                                                                                                          >  2:1495888/1‑140 (MQ=255)

               ggCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGAcc                                                                                                                        <  2:1344277/140‑1 (MQ=255)

                 cGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGTCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACcac                                                                                                                      <  1:1143257/140‑1 (MQ=255)

                 cGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACcac                                                                                                                      <  1:156127/140‑1 (MQ=255)

                   atatCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACAt                                                                                                                    <  1:183223/140‑1 (MQ=255)

                     atCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTg                                                                                                                  >  1:636870/1‑140 (MQ=255)

                     atCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTg                                                                                                                  >  2:1252361/1‑140 (MQ=255)

                        cGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGgtg                                                                                                               <  1:1156283/140‑1 (MQ=255)

                           gAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTgg                                                                                                            <  2:1137683/140‑1 (MQ=255)

                              ccAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCtg                                                                                                         >  1:1621900/1‑140 (MQ=255)

                               cAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCtgt                                                                                                        <  1:1263461/140‑1 (MQ=255)

                               cAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGCGTGGCtgt                                                                                                        >  1:137169/1‑140 (MQ=255)

                                aGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCtgtg                                                                                                       >  2:1567995/1‑140 (MQ=255)

                                aGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCtgtg                                                                                                       >  2:515584/1‑140 (MQ=255)

                                aGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCtgtg                                                                                                       >  2:56705/1‑140 (MQ=255)

                                aGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCtgtg                                                                                                       >  2:1169366/1‑140 (MQ=255)

                                    tttGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCaaa                                                                                                   <  1:1528103/140‑1 (MQ=255)

                                       gTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAAc                                                                                                >  1:547775/1‑140 (MQ=255)

                                            cttcCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAg                                                                                           >  1:744935/1‑140 (MQ=255)

                                            cttcCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGAGCAAAAACTCCAg                                                                                           >  1:794457/1‑140 (MQ=255)

                                                  ttGGCGACTGACAAATCCGTTTAATATCTTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATaaa                                                                                     >  1:914328/1‑140 (MQ=255)

                                                  ttGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATaaa                                                                                     >  1:1161806/1‑140 (MQ=255)

                                                   tGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATaaaa                                                                                    <  1:823420/140‑1 (MQ=255)

                                                    ggCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAAc                                                                                   >  1:490640/1‑140 (MQ=255)

                                                     gCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACg                                                                                  <  1:168000/140‑1 (MQ=255)

                                                      cGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGt                                                                                 >  1:815569/1‑140 (MQ=255)

                                                        aCTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTc                                                                               <  2:1621900/140‑1 (MQ=255)

                                                         cTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTcc                                                                              <  2:1172066/140‑1 (MQ=255)

                                                          tGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCg                                                                             <  1:452282/140‑1 (MQ=255)

                                                            aCAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGtt                                                                           <  1:1605475/140‑1 (MQ=255)

                                                                   cGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTcc                                                                    <  1:56705/140‑1 (MQ=255)

                                                                     tttAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCt                                                                  <  1:795518/140‑1 (MQ=255)

                                                                     tttAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCt                                                                  <  1:1065443/140‑1 (MQ=255)

                                                                      ttAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCtt                                                                 >  2:639148/1‑140 (MQ=255)

                                                                      ttAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCtt                                                                 <  2:399404/140‑1 (MQ=255)

                                                                      ttAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCtt                                                                 <  1:1495888/140‑1 (MQ=255)

                                                                         atatCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTtcat                                                              <  1:1274159/140‑1 (MQ=255)

                                                                             ccTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATa                                                          <  1:412097/140‑1 (MQ=255)

                                                                              cTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATAc                                                         <  2:1203495/140‑1 (MQ=255)

                                                                              cTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTCCCCTTCATCATAc                                                         <  2:1455210/140‑1 (MQ=255)

                                                                               tGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACa                                                        <  1:478290/140‑1 (MQ=255)

                                                                                ggAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAg                                                       <  2:1127060/140‑1 (MQ=255)

                                                                                ggAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAg                                                       <  2:1409723/140‑1 (MQ=255)

                                                                                 gAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCGCGGGCTGTTAGAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACCTGCCCTTCATCATACAGa                                                      <  2:750605/140‑1 (MQ=255)

                                                                                 gAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGa                                                      <  1:930123/140‑1 (MQ=255)

                                                                                 gAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGa                                                      <  2:807411/140‑1 (MQ=255)

                                                                                     aTGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGt                                                  <  1:839274/140‑1 (MQ=255)

                                                                                      tGGCGACCCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAATCTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt                                                 <  1:140511/140‑1 (MQ=255)

                                                                                      tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCCAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt                                                 >  2:1474568/1‑140 (MQ=255)

                                                                                      tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt                                                 <  1:1261294/140‑1 (MQ=255)

                                                                                      tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt                                                 <  1:1175619/140‑1 (MQ=255)

                                                                                      tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt                                                 >  2:114374/1‑140 (MQ=255)

                                                                                      tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt                                                 >  2:1340831/1‑140 (MQ=255)

                                                                                       ggCGAACCCGAGGGCATACGGGTTGATGCTGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTa                                                >  1:98737/1‑140 (MQ=255)

                                                                                       ggCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTa                                                >  1:1580302/1‑140 (MQ=255)

                                                                                       ggCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTa                                                >  2:553860/1‑140 (MQ=255)

                                                                                       ggCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTa                                                >  1:1503398/1‑140 (MQ=255)

                                                                                        gCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTaa                                               <  1:1541601/140‑1 (MQ=255)

                                                                                        gCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTaa                                               <  1:1252361/140‑1 (MQ=255)

                                                                                           aaCCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGa                                            <  2:636870/140‑1 (MQ=255)

                                                                                           aaCCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGa                                            <  1:315045/140‑1 (MQ=255)

                                                                                           aaCCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGa                                            >  2:1046909/1‑140 (MQ=255)

                                                                                           aaCCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGa                                            <  2:547775/140‑1 (MQ=255)

                                                                                           aaCCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGa                                            <  1:1189224/140‑1 (MQ=255)

                                                                                            aCCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGAt                                           >  2:520228/1‑140 (MQ=255)

                                                                                            aCCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGAt                                           <  2:878381/140‑1 (MQ=255)

                                                                                            aCCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGAt                                           <  2:511931/140‑1 (MQ=255)

                                                                                            aCCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGAt                                           <  1:867672/140‑1 (MQ=255)

                                                                                            aCCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGAt                                           <  1:1567995/140‑1 (MQ=255)

                                                                                                gAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGgtg                                       <  2:289026/140‑1 (MQ=255)

                                                                                                 aGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGgtgt                                      >  2:1143110/1‑140 (MQ=255)

                                                                                                   ggCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAg                                    <  1:842549/140‑1 (MQ=255)

                                                                                                        aCGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCa                               >  1:1371912/1‑140 (MQ=255)

                                                                                                        aCGGGTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCa                               >  1:1485506/1‑140 (MQ=255)

                                                                                                            gTTGATGCCGCTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAg                           <  2:744935/140‑1 (MQ=255)

                                                                                                                      cTGTACCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGCGTAGTGCCAGAAGGACGCCCAGc                 >  2:428946/1‑140 (MQ=255)

                                                                                                                          aCCACGGGCTGTTATAGGGG‑GGGCTGGAAGACCACATTGGGGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTc             >  2:1485408/1‑140 (MQ=255)

                                                                                                                               gggCTGTTATA‑GGGCGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTtca        <  1:1598644/140‑1 (MQ=255)

                                                                                                                                   tGTTATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTTCATCAc    <  1:114374/140‑1 (MQ=255)

                                                                                                                                     ttATAGGGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTTCATCACCt  >  1:1449655/1‑140 (MQ=255)

CAGCCAGTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑GGG‑GGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTTCATCACCT  >  NC_000913/1236135‑1236406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A