Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 162 93.0 3098984 99.0% 3067994 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,236,881 C→T A121T (GCT→ACT)  ycgB ← PF04293 family protein YcgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,236,8810CT98.7% 253.2 / NA 74A121T (GCT→ACT) ycgBPF04293 family protein YcgB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (38/35);  minor base A (1/0);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTCTTCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAAC  >  NC_000913/1236754‑1237011
                                                                                                                               |                                                                                                                                  
cGCTCTTCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAAGCGAACTGGCGTCGGTCCAGCTACGGAATAAGGAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAgcgc                                                                                                                        <  2:1363893/140‑1 (MQ=255)
   tctTCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTg                                                                                                                     >  1:823224/1‑140 (MQ=255)
     ttCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCa                                                                                                                   >  2:1209580/1‑140 (MQ=255)
     ttCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCa                                                                                                                   <  2:759782/140‑1 (MQ=255)
     ttCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCa                                                                                                                   <  1:252496/140‑1 (MQ=255)
       cGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATg                                                                                                                 >  2:359910/1‑140 (MQ=255)
       cGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATg                                                                                                                 >  1:1422432/1‑140 (MQ=255)
          aCTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTa                                                                                                              >  1:1125081/1‑140 (MQ=255)
           cTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTaa                                                                                                             <  2:138928/140‑1 (MQ=255)
             cGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATg                                                                                                           >  1:448058/1‑140 (MQ=255)
             cGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATg                                                                                                           >  1:930161/1‑140 (MQ=255)
                tAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGgtg                                                                                                        <  2:1422432/140‑1 (MQ=255)
                    atatTTACGGGCAAAAATCAGATAGTCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTtct                                                                                                    <  2:1507263/140‑1 (MQ=255)
                    atatTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTtct                                                                                                    <  1:13629/140‑1 (MQ=255)
                     tatTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTtctc                                                                                                   >  1:406486/1‑140 (MQ=255)
                        ttACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGGATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTc                                                                                                >  2:1135175/1‑140 (MQ=255)
                        ttACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTc                                                                                                >  2:1040586/1‑140 (MQ=255)
                         tACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTcc                                                                                               >  1:1445286/1‑140 (MQ=255)
                         tACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTcc                                                                                               <  1:905210/140‑1 (MQ=255)
                          aCGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCa                                                                                              >  1:1368394/1‑140 (MQ=255)
                          aCGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCa                                                                                              >  1:825784/1‑140 (MQ=255)
                          aCGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCa                                                                                              >  2:549037/1‑140 (MQ=255)
                          aCGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCa                                                                                              >  2:1039578/1‑140 (MQ=255)
                          aCGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCa                                                                                              >  2:778045/1‑140 (MQ=255)
                          aCGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCa                                                                                              >  2:185109/1‑140 (MQ=255)
                           cGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCAt                                                                                             <  2:740837/140‑1 (MQ=255)
                             ggCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCa                                                                                           >  2:893932/1‑140 (MQ=255)
                             ggCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCa                                                                                           >  1:1069479/1‑140 (MQ=255)
                             ggCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCa                                                                                           >  1:1130868/1‑140 (MQ=255)
                              gCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAg                                                                                          >  2:1547662/1‑140 (MQ=255)
                               cAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAgg                                                                                         >  1:698846/1‑140 (MQ=255)
                                 aaaaTCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTa                                                                                       >  2:1272326/1‑140 (MQ=255)
                                     tCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCg                                                                                   <  2:120334/140‑1 (MQ=255)
                                        gATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGCATAAGTAATTGTTTTTGAAGAAAGAGTTACGCCCATAGCAGGCATGATACATCACCAGCGCTTGCATGGCAATGGTGTTCTCTTCCATCAGGTAAGCGATa                                                                                >  1:147390/1‑140 (MQ=255)
                                         aTAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATac                                                                               <  1:1209580/140‑1 (MQ=255)
                                         aTAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATac                                                                               <  2:1125081/140‑1 (MQ=255)
                                          tAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATaca                                                                              <  1:1272326/140‑1 (MQ=255)
                                          tAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATaca                                                                              <  2:75300/140‑1 (MQ=255)
                                          tAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATaca                                                                              <  2:683904/140‑1 (MQ=255)
                                          tAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATaca                                                                              <  1:457421/140‑1 (MQ=255)
                                            aTCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACg                                                                            >  1:875178/1‑140 (MQ=255)
                                            aTCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACg                                                                            <  2:1193895/140‑1 (MQ=255)
                                             tCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACgg                                                                           >  1:1028747/1‑140 (MQ=255)
                                                aCAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGtt                                                                        >  2:1131037/1‑140 (MQ=255)
                                                   aTCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTaga                                                                     >  1:949561/1‑140 (MQ=255)
                                                   aTCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTaga                                                                     >  1:324233/1‑140 (MQ=255)
                                                    tCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTagag                                                                    <  2:744939/140‑1 (MQ=255)
                                                       aaCTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTa                                                                 >  1:1020085/1‑140 (MQ=255)
                                                         cTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAAt                                                               <  2:1460960/140‑1 (MQ=255)
                                                            gCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGAc                                                            >  2:1328543/1‑140 (MQ=255)
                                                             cGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGGGTTAATGACg                                                           <  2:930161/140‑1 (MQ=255)
                                                              gTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGa                                                          <  1:1009038/140‑1 (MQ=255)
                                                              gTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGa                                                          <  1:366437/140‑1 (MQ=255)
                                                                  gTCCAGCTAAGGAAAAAGTAATTGTTTTTGAAGAAAGAGTTAAGCCCATAGCAGGCATGAGTCCTCACCATCGCTTTTATGTAAATGGGGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTc                                                      >  2:698265/1‑140 (MQ=255)
                                                                   tCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCa                                                     <  1:549037/140‑1 (MQ=255)
                                                                       gCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAgg                                                 <  2:698846/140‑1 (MQ=255)
                                                                        cTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGc                                                <  2:406486/140‑1 (MQ=255)
                                                                         tACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGcc                                               <  1:240988/140‑1 (MQ=255)
                                                                             gAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTc                                           >  2:308337/1‑140 (MQ=255)
                                                                             gAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTc                                           >  2:1312138/1‑140 (MQ=255)
                                                                                   gTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCt                                     >  1:963372/1‑140 (MQ=255)
                                                                                      aTTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCCCCAGCGCTTGCATGGTAATGGCGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGAc                                  <  2:1021317/140‑1 (MQ=255)
                                                                                      aTTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGAc                                  <  1:185109/140‑1 (MQ=255)
                                                                                      aTTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGAc                                  <  1:1040586/140‑1 (MQ=255)
                                                                                      aTTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGAc                                  <  1:1135175/140‑1 (MQ=255)
                                                                                      aTTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGAc                                  <  1:1547662/140‑1 (MQ=255)
                                                                                      aTTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGAc                                  <  2:823224/140‑1 (MQ=255)
                                                                                      aTTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGAc                                  <  1:359910/140‑1 (MQ=255)
                                                                                      aTTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGAc                                  <  1:778045/140‑1 (MQ=255)
                                                                                        tGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCg                                >  1:1345131/1‑140 (MQ=255)
                                                                                        tGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCg                                >  2:780317/1‑140 (MQ=255)
                                                                                         gTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGt                               <  1:1131037/140‑1 (MQ=255)
                                                                                                   aaagaGTTATGCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATTGTGTTCTCTTCCATCAGGTAAGCGTTACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAg                     <  2:448058/140‑1 (MQ=255)
                                                                                                             gCCCATAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGtc           >  2:113702/1‑140 (MQ=255)
                                                                                                                 aTAGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGa       >  2:1179669/1‑140 (MQ=255)
                                                                                                                   aGCAGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATa     >  2:297663/1‑140 (MQ=255)
                                                                                                                      aGGCATGAGTCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAAc  <  2:1130868/140‑1 (MQ=255)
                                                                                                                               |                                                                                                                                  
CGCTCTTCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAAC  >  NC_000913/1236754‑1237011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A