breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 2,799 A→G *821W (TGA→TGG thrA → fused aspartate kinase/homoserine dehydrogenase 1
RA 9,905 (C)6→7 intergenic (+12/+23) mog → / ← satP molybdopterin adenylyltransferase/acetate/succinate:H(+) symporter
RA 86,183 A→G Y185C (TAC→TGC)  ilvI → acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
RA 101,615 T→C V284A (GTC→GCC)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase
RA 103,419 A→G T89A (ACC→GCC)  ftsQ → cell division protein FtsQ
RA 108,767 A→G A163A (GCA→GCG secA → protein translocation ATPase
RA 112,234 A→G R122R (CGT→CGC zapD ← cell division factor ZapD
RA 150,021 A→G G311G (GGT→GGC yadC ← fimbrial tip‑adhesin YadC
RA 195,993 A→G Y106C (TAC→TGC)  cdsA → CDP‑diglyceride synthetase
RA 216,985 A→G Y7H (TAC→CAC)  yaeF ← peptidase C92 family protein
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 268,509 A→G V151A (GTT→GCT)  ykfA ← CP4‑6 prophage; putative GTP‑binding protein YkfA
RA 278,775 G→A R10C (CGC→TGC)  afuC ← CP4‑6 prophage; ABC transporter ATP‑binding protein AfuC
RA 279,935 A→G intergenic (‑60/+450) insA‑2 ← / ← yagB IS1 protein InsA/orphan antitoxin YagB
RA 283,807 A→G T203A (ACC→GCC)  yagF → CP4‑6 prophage; D‑xylonate dehydratase
RA 287,576 A→G Y263C (TAC→TGC)  yagH → CP4‑6 prophage; putative xylosidase/arabinosidase
RA 292,395 T→C Y185C (TAC→TGC)  yagK ← CP4‑6 prophage; uncharacterized protein YagK
RA 303,093 (G)6→7 coding (103/615 nt) yagU → inner membrane protein that contributes to acid resistance
RA 324,995 C→T A124T (GCC→ACC)  ykgH ← uncharacterized protein YkgH
RA 337,584 A→G E269E (GAA→GAG yahF → putative acyl‑CoA synthetase YahF
RA 375,721 C→T A88V (GCG→GTG)  mhpT → 3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 387,582 T→C R204R (CGT→CGC tauC → taurine ABC transporter membrane subunit
RA 407,543 A→G K39R (AAG→AGG)  aroM → protein AroM
RA 415,717 A→G S13P (TCA→CCA)  sbcC ← ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
RA 459,368 G→A D161N (GAT→AAT)  lon → Lon protease
RA 506,791 A→G Y98H (TAC→CAC)  ybaK ← Cys‑tRNA(Pro) and Cys‑tRNA(Cys) deacylase
RA 512,835 A→G N87S (AAC→AGC)  ybaT → putative transporter YbaT
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 670,318 A→G T85T (ACT→ACC nadD ← nicotinate‑mononucleotide adenylyltransferase
JC JC 679,481 IS5 (–) +4 bp intergenic (‑75/‑24) ybeT ← / → ybeU Sel1 repeat‑containing protein YbeT/DUF1266 domain‑containing protein YbeU
RA 833,991 T→C C308R (TGT→CGT)  dinG → ATP‑dependent DNA helicase DinG
RA 837,442 (A)8→7 intergenic (+6/+223) hcxB → / ← ybiJ hydroxycarboxylate dehydrogenase B/DUF1471 domain‑containing protein YbiJ
RA 857,914 (T)5→4 coding (1148/1266 nt) ybiU ← DUF1479 domain‑containing protein YbiU
RA 884,345 (G)5→4 coding (673/1233 nt) mdfA → multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 894,476 C→T N231N (AAC→AAT potF → putrescine ABC transporter periplasmic binding protein
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 934,792 A→G E523E (GAA→GAG ftsK → cell division DNA translocase FtsK
RA 943,068 T→G S704A (TCT→GCT)  dmsA → dimethyl sulfoxide reductase subunit A
RA 952,685 A→G R290R (CGT→CGC pflB ← pyruvate formate‑lyase
RA 974,067 A→G K177K (AAA→AAG cmoM → tRNA cmo(5)U34 methyltransferase
RA 976,235 T→C I205T (ATT→ACT)  mukE → chromosome partitioning protein MukE
RA 1,001,023 (A)8→7 coding (1808/2601 nt) elfC → putative fimbrial usher protein ElfC
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,029,454 Δ1 bp coding (516/1191 nt) rlmI ← 23S rRNA m(5)C1962 methyltransferase
RA 1,064,804 A→G T257A (ACG→GCG)  yccE → uncharacterized protein YccE
RA 1,066,190 T→C C202C (TGT→TGC agp → glucose‑1‑phosphatase
RA 1,129,332 A→G V498V (GTA→GTG murJ → putative lipid II flippase MurJ
RA 1,136,798 G→A L175L (TTG→TTA flgI → flagellar P‑ring protein
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,200,747 A→G S20S (AGT→AGC intE ← e14 prophage; putative integrase
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,236,881 C→T A121T (GCT→ACT)  ycgB ← PF04293 family protein YcgB
RA 1,237,865 A→G S99G (AGC→GGC)  dadA → D‑amino acid dehydrogenase
RA 1,260,895 (A)7→8 intergenic (‑92/+33) dauA ← / ← prs aerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase
RA 1,274,761 T→C S326S (AGT→AGC ychO → putative invasin YchO
RA 1,295,487 C→T A879T (GCT→ACT)  adhE ← aldehyde‑alcohol dehydrogenase
RA 1,319,831 A→G G518G (GGT→GGC trpD ← anthranilate synthase subunit TrpD
RA 1,348,839 A→G V25A (GTG→GCG)  rnb ← RNase II
RA 1,353,137 A→G C164C (TGT→TGC sapD ← putrescine ABC exporter ATP binding protein SapD
RA 1,360,148 A→G L254P (CTG→CCG)  puuA ← glutamate‑putrescine ligase
RA 1,397,844 T→C H260R (CAC→CGC)  uspE ← universal stress protein with a role cellular motility
RA 1,400,524 A→G V417A (GTA→GCA)  abgT ← p‑aminobenzoyl glutamate:H(+) symporter
RA 1,438,355 T→C T810T (ACA→ACG ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,474,342 T→C V41A (GTG→GCG)  pdxI → pyridoxine 4‑dehydrogenase
RA 1,505,496 T→G K195N (AAA→AAC ydcO ← putative transport protein YdcO
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,570,413 A→G V26A (GTA→GCA)  gadC ← L‑glutamate:4‑aminobutyrate antiporter
RA 1,588,201 A→G G32G (GGT→GGC ydeR ← putative fimbrial protein YdeR
RA 1,594,131 (C)8→6 pseudogene (3955‑3956/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,604,295 T→C G83G (GGT→GGC lsrD → Autoinducer‑2 ABC transporter membrane subunit LsrD
RA 1,631,706 T→C N194D (AAC→GAC)  ydfJ ← putative transporter YdfJ
RA 1,633,705 T→C G28G (GGT→GGC pinQ → Qin prophage; putative site‑specific recombinase
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,658,260 T→C C64C (TGT→TGC ynfE → putative selenate reductase YnfE
RA 1,661,914 T→C G453G (GGT→GGC ynfF → putative selenate reductase YnfF
RA 1,674,092 G→A P60P (CCG→CCA tqsA → autoinducer 2 exporter
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,703,538 T→C E257E (GAA→GAG ydgJ ← putative oxidoreductase YdgJ
RA 1,704,559 T→C R3R (CGT→CGC blr → beta‑lactam resistance protein
RA 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
RA 1,750,257 A→T I31N (ATC→AAC)  ydhU ← putative cytochrome YdhU
RA 1,771,945 T→C V292A (GTC→GCC)  ydiM → putative exporter YdiM
RA 1,806,470 T→C V34A (GTG→GCG)  pfkB → 6‑phosphofructokinase II
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,883,729 C→T S181F (TCC→TTC)  yeaV → putative transporter YeaV
RA 1,928,893 G→A intergenic (‑54/+155) ptrB ← / ← yebE oligopeptidase B/conserved inner membrane protein YebE
RA 1,933,631 T→C D325G (GAC→GGC)  edd ← phosphogluconate dehydratase
RA 1,959,168 (C)6→7 coding (99/747 nt) cutC ← protein CutC
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,987,599 G→A P54S (CCC→TCC)  yecJ ← DUF2766 domain‑containing protein YecJ
RA 1,996,183 T→C N217S (AAC→AGC)  sdiA ← DNA‑binding transcriptional dual regulator SdiA
RA 2,015,321 T→C L148L (TTG→CTG)  fliG → flagellar motor switch protein FliG
RA 2,045,291 G→A P118P (CCG→CCA yeeJ → inverse autotransporter adhesin
RA 2,087,633 G→A T209M (ACG→ATG)  yeeY ← putative DNA‑binding transcriptional regulator YeeY
RA 2,166,877 C→T H404Y (CAC→TAC)  yegQ → putative peptidase YegQ
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,227,441 A→G pseudogene (1318/1436 nt) mdtQ ← putative multidrug resistance outer membrane protein MdtQ
RA 2,273,000 T→C Y213H (TAC→CAC)  yejA → putative oligopeptide ABC transporter periplasmic binding protein
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,532,596 T→C V63A (GTT→GCT)  cysK → O‑acetylserine sulfhydrylase A
RA 2,536,991 G→A R83C (CGT→TGT)  pdxK ← pyridoxal kinase I
RA 2,546,759 T→C C29C (TGT→TGC murP → N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
RA 2,585,922 T→C S64S (AGT→AGC narQ → sensory histidine kinase NarQ
RA 2,588,164 G→A P190P (CCG→CCA acrD → multidrug efflux pump RND permease AcrD
RA 2,735,510 T→C T168A (ACT→GCT)  rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase
RA 2,744,933 T→C Y136C (TAC→TGC)  trmD ← tRNA m(1)G37 methyltransferase
RA 2,760,492 T→C intergenic (+98/‑55) yfjI → / → yfjJ CP4‑57 prophage; protein YfjI/CP4‑57 prophage; protein YfjJ
RA 2,782,804 A→G intergenic (‑78/+261) ypjA ← / ← pinH adhesin‑like autotransporter YpjA/putative invertase fragment
RA 2,794,219 T→C intergenic (+204/‑34) gabT → / → gabP 4‑aminobutyrate aminotransferase GabT/4‑aminobutyrate:H(+) symporter
RA 2,798,813 A→G intergenic (‑318/‑351) stpA ← / → alaE DNA‑binding transcriptional repressor StpA with RNA chaperone activity/L‑alanine exporter
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,821,878 T→C N45S (AAC→AGC)  alaS ← alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,865,053 (C)6→5 intergenic (+41/‑48) ygbM → / → ygbN putative 2‑oxo‑tetronate isomerase YgbM/putative transporter YgbN
RA 2,927,799 T→C intergenic (+127/‑430) ppnN → / → sdaC nucleotide 5'‑monophosphate nucleosidase/serine:H(+) symporter SdaC
RA 2,942,901 (T)8→7 intergenic (+1/+17) gcvB → / ← ygdI small regulatory RNA GcvB/DUF903 domain‑containing lipoprotein YgdI
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,977,716 A→G V395A (GTT→GCT)  lysA ← diaminopimelate decarboxylase
RA 2,993,351 C→T P420S (CCT→TCT)  ygeH → putative transcriptional regulator YgeH
RA 3,028,144 A→G T342A (ACC→GCC)  ghxQ → guanine/hypoxanthine transporter GhxQ
RA 3,059,205 T→C T41A (ACA→GCA)  rpiA ← ribose‑5‑phosphate isomerase A
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,080,992 T→C D215G (GAC→GGC)  tktA ← transketolase 1
RA 3,153,184 A→G K317E (AAA→GAA)  metC → cystathionine beta‑lyase/L‑cysteine desulfhydrase
RA 3,240,945 T→C A334A (GCT→GCC sstT → serine/threonine:Na(+) symporter
RA 3,287,400 T→C intergenic (+375/‑26) agaI → / → yraH putative deaminase AgaI/putative fimbrial protein YraH
RA 3,297,389 (G)5→6 coding (750/1041 nt) yraQ ← permease family protein YraQ
RA 3,334,125 T→C V139A (GTA→GCA)  ispB → all‑trans‑octaprenyl‑diphosphate synthase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,375,556 C→T Q230* (CAG→TAG)  dcuD → putative transporter DcuD
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,397,734 C→T G21D (GGC→GAC)  rng ← RNase G
RA 3,403,827 G→A G115E (GGG→GAG)  yhdH → acrylyl‑CoA reductase
RA 3,445,283 T→C A105A (GCA→GCG rplR ← 50S ribosomal subunit protein L18
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,613,291 T→C V108A (GTA→GCA)  acpT → holo‑[acyl carrier protein] synthase 2
RA 3,795,090 T→C A54A (GCT→GCC waaF → ADP‑heptose‑‑LPS heptosyltransferase 2
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,884,188 T→C intergenic (+82/‑148) ysdD → / → rpmH protein YsdD/50S ribosomal subunit protein L34
RA 3,934,828 A→G M351V (ATG→GTG)  rbsA → ribose ABC transporter ATP binding subunit
RA 4,019,831 A→G T70A (ACC→GCC)  ubiJ → ubiquinone biosynthesis accessory factor UbiJ
RA 4,152,699 C→T V134I (GTC→ATC)  ppc ← phosphoenolpyruvate carboxylase
RA 4,295,029 A→G E183E (GAA→GAG gltP → glutamate/aspartate : H(+) symporter GltP
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,304,147 A→G F86S (TTC→TCC)  ytcA ← putative lipoprotein YtcA
MC 4,326,086 Δ100 bp between other yjdN ← / ← yjdM conserved protein YjdN/conserved protein YjdM
RA 4,361,448 (A)8→7 coding (487/1539 nt) cadC ← DNA‑binding transcriptional activator CadC
RA 4,377,043 A→G T57A (ACG→GCG)  gdx → guanidinium exporter
RA 4,391,437 A→G S25P (TCT→CCT)  rsgA ← ribosome small subunit‑dependent GTPase A
RA 4,417,416 A→G I155M (ATA→ATG yjfP → carboxylesterase
RA 4,426,751 A→G G224G (GGT→GGC yjfZ ← protein YjfZ
RA 4,433,902 C→T L41L (CTG→CTA qorB ← NAD(P)H:quinone oxidoreductase
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,466,573 A→G V225A (GTT→GCT)  treR ← DNA‑binding transcriptional repressor TreR
RA 4,539,762 A→G intergenic (‑261/‑1195) nanC ← / → fimB N‑acetylneuraminic acid outer membrane channel/regulator for fimA
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,639,212 A→G E345E (GAA→GAG creD → putative inner membrane protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 84019 84151 133 30 [29] [29] 30 leuL/leuO leu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO
* * ÷ NC_000913 1806194 1806365 172 32 [27] [28] 30 ydiY/pfkB acid‑inducible putative outer membrane protein YdiY/6‑phosphofructokinase II
* * ÷ NC_000913 2033785 2033975 191 33 [29] [27] 30 rseX/yedS small regulatory RNA RseX/putative outer membrane protein YedS, N‑terminal fragment
* * ÷ NC_000913 2249608 2249711 104 33 [29] [24] 30 [yeiE] [yeiE]
* * ÷ NC_000913 3413534 3413663 130 30 [29] [29] 30 envR/acrE DNA‑binding transcriptional repressor EnvR/multidrug efflux pump membrane fusion lipoprotein AcrE
* * ÷ NC_000913 3423770–3424234 3424558–3424238 5–789 34 [24] [28] 40 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3751874 3752017 144 30 [29] [29] 35 [yiaU] [yiaU]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =30 (0.330)67 (0.830) 38/246 0.8 75.9% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 16 (0.200)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780530 (0.330)54 (0.670) 40/246 0.6 71.8% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 16 (0.200)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)48 (0.540) 32/270 1.5 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =106 (1.160)140 (1.540) 73/276 0.0 72.1% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)100 (1.090) 55/278 0.3 97.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA