Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,296,985	C→T	P379P (CCG→CCA)	adhE ←	aldehyde‑alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,296,985	0	C	T	97.8%	152.0 / ‑5.5	45	P379P (CCG→CCA)	adhE	aldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (22/22); total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTC  >  NC_000913/1296849‑1297119

cccAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTggt                                                                                                                                     >  2:48578/1‑140 (MQ=255)

     gAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTggttggtt                                                                                                                                >  1:626900/1‑140 (MQ=255)

     gAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTggttgg                                                                                                                                  >  2:672754/1‑138 (MQ=255)

     gAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTggttgg                                                                                                                                  <  1:672754/138‑1 (MQ=255)

             aCCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGt                                                                                                                        <  1:136330/140‑1 (MQ=255)

             aCCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGt                                                                                                                        <  2:396665/140‑1 (MQ=255)

              cccAGAGCCAGGGAAGGCGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTc                                                                                                                       >  1:736876/1‑140 (MQ=255)

                cAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGt                                                                                                                        <  2:698466/137‑1 (MQ=255)

                cAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGt                                                                                                                        >  1:698466/1‑137 (MQ=255)

                cAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAg                                                                                                                     <  2:626900/140‑1 (MQ=255)

                  gagTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAgtg                                                                                                                   <  2:1047196/140‑1 (MQ=255)

                    gTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTa                                                                                                                 <  2:210446/140‑1 (MQ=255)

                      cAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACa                                                                                                               <  2:349945/140‑1 (MQ=255)

                         ggAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGc                                                                                                            >  2:835882/1‑140 (MQ=255)

                          gAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCa                                                                                                           <  1:817700/140‑1 (MQ=255)

                           aaGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCaa                                                                                                          <  1:306105/140‑1 (MQ=255)

                               tGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAgg                                                                                                      >  2:125838/1‑140 (MQ=255)

                                gCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGt                                                                                                     <  1:48578/140‑1 (MQ=255)

                                gCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGt                                                                                                     <  2:62636/140‑1 (MQ=255)

                                   aGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATg                                                                                                  >  1:640955/1‑140 (MQ=255)

                                         aaGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGAt                                                                                            >  1:1055589/1‑140 (MQ=255)

                                          aGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATa                                                                                           <  2:867052/140‑1 (MQ=255)

                                                    gTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAg                                                                                 >  1:293004/1‑140 (MQ=255)

                                                     tCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGc                                                                                >  2:1057192/1‑140 (MQ=255)

                                                              ccaccCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGttt                                                                       >  2:263235/1‑140 (MQ=255)

                                                                 cccTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTtctc                                                                    >  1:270212/1‑140 (MQ=255)

                                                                  ccTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTctct                                                                   <  2:1055589/140‑1 (MQ=255)

                                                                      agaCGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCtt                                                               >  2:772765/1‑140 (MQ=255)

                                                                        aCGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCtttt                                                             >  2:40463/1‑140 (MQ=255)

                                                                            tGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTa                                                         >  2:282077/1‑140 (MQ=255)

                                                                                                 cgcCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTa                                     <  2:397103/139‑1 (MQ=255)

                                                                                                 cgcCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTa                                     >  1:397103/1‑139 (MQ=255)

                                                                                                    cGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAgcgc                                 <  1:282077/140‑1 (MQ=255)

                                                                                                    cGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAgcgc                                 <  1:125838/140‑1 (MQ=255)

                                                                                                     gTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTc                                               >  2:168325/1‑125 (MQ=255)

                                                                                                     gTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTc                                               <  1:168325/125‑1 (MQ=255)

                                                                                                     gTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAgcg                                  >  1:783366/1‑138 (MQ=255)

                                                                                                     gTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAgcg                                  <  2:783366/138‑1 (MQ=255)

                                                                                                     gTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGcgcg                                >  2:738663/1‑140 (MQ=255)

                                                                                                     gTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGACCAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGcgcg                                >  1:266726/1‑140 (MQ=255)

                                                                                                        ttCATTTTCTGACCGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTa                             >  1:228855/1‑140 (MQ=255)

                                                                                                                     cGAAGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTc                <  1:301626/140‑1 (MQ=255)

                                                                                                                       aaGTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCgg              >  1:237666/1‑140 (MQ=255)

                                                                                                                         gTAAGAAACGCGAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCgggg            <  2:504387/140‑1 (MQ=255)

                                                                                                                                   cgAGCTGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTc  <  1:1057192/140‑1 (MQ=255)

CCCAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTC  >  NC_000913/1296849‑1297119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A