Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F28 I1 R1 134 60.5 2175152 94.0% 2044642 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,479,590 A→G A151A (GCA→GCG)  ynbC → hydrolase/methyltransferase domain‑containing protein YnbC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,479,5900AG100.0% 203.0 / NA 57A151A (GCA→GCG) ynbChydrolase/methyltransferase domain‑containing protein YnbC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/28);  total (29/28)

GGTGGTAGTGATCGCGCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATT  >  NC_000913/1479461‑1479725
                                                                                                                                 |                                                                                                                                       
ggtggtAGTGATCGCGCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTgcgc                                                                                                                               >  1:559548/1‑140 (MQ=255)
       gTGATCGCGCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGtt                                                                                                                        <  2:559548/140‑1 (MQ=255)
          aTCGCGCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATg                                                                                                                     >  1:668403/1‑140 (MQ=255)
           tCGCGCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATgg                                                                                                                    >  2:814640/1‑140 (MQ=255)
            cgcgCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGc                                                                                                                   <  2:730725/140‑1 (MQ=255)
            cgcgCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGc                                                                                                                   >  1:431193/1‑140 (MQ=255)
              cgcAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGtt                                                                                                                        >  1:1068443/1‑133 (MQ=255)
              cgcAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGtt                                                                                                                        <  2:1068443/133‑1 (MQ=255)
              cgcAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGtt                                                                                                                        >  2:628932/1‑133 (MQ=255)
              cgcAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGtt                                                                                                                        <  1:628932/133‑1 (MQ=255)
               gcAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATc                                                                                                                >  1:629834/1‑140 (MQ=255)
                    gCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTg                                                                                                           >  2:633069/1‑140 (MQ=255)
                     cGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGc                                                                                                          <  1:3688/140‑1 (MQ=255)
                      gTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCg                                                                                                         <  1:491646/140‑1 (MQ=255)
                          gcgcAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGt                                                                                                     <  1:1029500/140‑1 (MQ=255)
                                      ttGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTttatt                                                                                         <  1:894028/140‑1 (MQ=255)
                                        gCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTaa                                                                                       <  1:747024/140‑1 (MQ=255)
                                          cacaTGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAAtt                                                                                     >  1:339792/1‑140 (MQ=255)
                                          cacaTGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAAtt                                                                                     >  2:1046584/1‑140 (MQ=255)
                                              tGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTa                                                                                 >  2:29019/1‑140 (MQ=255)
                                                    cATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGaa                                                                           <  2:227680/140‑1 (MQ=255)
                                                    cATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGaa                                                                           <  1:633069/140‑1 (MQ=255)
                                                    cATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGaa                                                                           <  1:896203/140‑1 (MQ=255)
                                                        aTTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGa                                                                       <  1:814640/140‑1 (MQ=255)
                                                        aTTATGCACCGGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGa                                                                       >  2:571869/1‑140 (MQ=255)
                                                           aTGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGa                                                                       >  1:75048/1‑137 (MQ=255)
                                                           aTGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGa                                                                       <  2:75048/137‑1 (MQ=255)
                                                           aTGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGc                                                                    >  2:791463/1‑140 (MQ=255)
                                                                  tGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGa                                                             <  1:791463/140‑1 (MQ=255)
                                                                   gCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACg                                                                     >  1:272261/1‑131 (MQ=255)
                                                                   gCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACg                                                                     <  2:272261/131‑1 (MQ=255)
                                                                   gCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGAc                                                            >  1:698713/1‑140 (MQ=255)
                                                                   gCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGAc                                                            >  1:800651/1‑140 (MQ=255)
                                                                    cAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGAcc                                                           >  1:70561/1‑140 (MQ=255)
                                                                      aTTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCa                                                         >  1:211712/1‑140 (MQ=255)
                                                                      aTTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCa                                                         <  1:571869/140‑1 (MQ=255)
                                                                          gcgGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGAt                                                     <  1:821883/140‑1 (MQ=255)
                                                                                  gTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACg                                             >  2:963204/1‑140 (MQ=255)
                                                                                       ggCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACgggggg                                        <  2:698713/140‑1 (MQ=255)
                                                                                        gCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGc                                       >  2:498346/1‑140 (MQ=255)
                                                                                         cTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCg                                      <  2:431193/140‑1 (MQ=255)
                                                                                            ctcCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGt                                   <  1:366901/140‑1 (MQ=255)
                                                                                             tcCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGtt                                  >  2:927774/1‑140 (MQ=255)
                                                                                              ccGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGttt                                 <  2:1037515/140‑1 (MQ=255)
                                                                                               cGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTc                                <  1:1046584/140‑1 (MQ=255)
                                                                                                 gCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCaa                              <  1:29019/140‑1 (MQ=255)
                                                                                                  ccTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCaat                             >  2:1074884/1‑140 (MQ=255)
                                                                                                   cTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCaata                            <  1:1074884/140‑1 (MQ=255)
                                                                                                       aaGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGa                        >  2:453555/1‑140 (MQ=255)
                                                                                                         ggTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATc                      <  1:498346/140‑1 (MQ=255)
                                                                                                            tAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGc                   >  2:342124/1‑140 (MQ=255)
                                                                                                             aaaTTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCt                  <  2:629834/140‑1 (MQ=255)
                                                                                                             aaaTTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCt                  >  2:843082/1‑140 (MQ=255)
                                                                                                                    aTGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATcaca           >  1:654596/1‑140 (MQ=255)
                                                                                                                          cGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCg     >  2:510731/1‑140 (MQ=255)
                                                                                                                             tGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGAtt  <  2:70561/140‑1 (MQ=255)
                                                                                                                             tGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGAtt  >  1:27295/1‑140 (MQ=255)
                                                                                                                                 |                                                                                                                                       
GGTGGTAGTGATCGCGCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATT  >  NC_000913/1479461‑1479725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A