Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F28 I1 R1 134 60.5 2175152 94.0% 2044642 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,719,230 T→C T125A (ACC→GCC)  anmK ← anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,719,2300TC100.0% 205.1 / NA 58T125A (ACC→GCC) anmKanhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/30);  total (28/30)

CCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCA  >  NC_000913/1719097‑1719364
                                                                                                                                     |                                                                                                                                      
ccAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGgcgcgtgc                                                                                                                                  <  2:512817/140‑1 (MQ=255)
  aaTATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCca                                                                                                                                <  2:581452/140‑1 (MQ=255)
   atatTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCcac                                                                                                                               <  2:816833/140‑1 (MQ=255)
        gAGCACCATTCGTCACTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAAttt                                                                                                                          >  1:831151/1‑140 (MQ=255)
             ccATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTg                                                                                                                     >  1:62372/1‑140 (MQ=255)
              cATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGt                                                                                                                    <  2:849067/140‑1 (MQ=255)
               aTTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGtt                                                                                                                   >  2:407712/1‑140 (MQ=255)
                ttCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTa                                                                                                                  >  2:1047650/1‑140 (MQ=255)
                ttCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTa                                                                                                                  >  2:889192/1‑140 (MQ=255)
                     cgCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGcc                                                                                                             <  1:655676/140‑1 (MQ=255)
                     cgCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGcc                                                                                                             <  1:1047650/140‑1 (MQ=255)
                          ggTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCt                                                                                                        >  2:301050/1‑140 (MQ=255)
                                    gCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAgtgtg                                                                                              >  1:684814/1‑140 (MQ=255)
                                     ccAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAgtgtgt                                                                                             <  1:407712/140‑1 (MQ=255)
                                     ccAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAgtgtgt                                                                                             <  1:640977/140‑1 (MQ=255)
                                     ccAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAgtgtgt                                                                                             >  1:140115/1‑140 (MQ=255)
                                      cagcagCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGCAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGtgtgtg                                                                                            >  2:163319/1‑140 (MQ=255)
                                         cagcGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAgtgtg                                                                                              >  2:994664/1‑135 (MQ=255)
                                         cagcGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAgtgtg                                                                                              <  1:994664/135‑1 (MQ=255)
                                                   tgGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGt                                                                               <  2:191461/140‑1 (MQ=255)
                                                   tgGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGt                                                                               <  1:140959/140‑1 (MQ=255)
                                                       aCGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGt                                                                           <  2:799810/140‑1 (MQ=255)
                                                       aCGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGt                                                                           <  2:831151/140‑1 (MQ=255)
                                                       aCGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGt                                                                           <  1:301050/140‑1 (MQ=255)
                                                           aGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCAt                                                                       >  2:534759/1‑140 (MQ=255)
                                                           aGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCAt                                                                       >  1:443627/1‑140 (MQ=255)
                                                             gTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGc                                                                     >  2:533612/1‑140 (MQ=255)
                                                               aCCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCa                                                                   <  2:62372/140‑1 (MQ=255)
                                                                       tGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGttt                                                           >  2:1084873/1‑140 (MQ=255)
                                                                       tGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGttt                                                           >  1:454348/1‑140 (MQ=255)
                                                                        gcgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTg                                                          <  1:1084873/140‑1 (MQ=255)
                                                                        gcgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTg                                                          >  2:259281/1‑140 (MQ=255)
                                                                         cgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGa                                                                 >  1:94410/1‑132 (MQ=255)
                                                                         cgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGa                                                                 <  2:94410/132‑1 (MQ=255)
                                                                         cgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGa                                                         >  1:470744/1‑140 (MQ=255)
                                                                           ccTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGAcc                                                       >  1:270304/1‑140 (MQ=255)
                                                                              tGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTg                                                    >  1:402263/1‑140 (MQ=255)
                                                                                    cccAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAAcc                                              >  1:826947/1‑140 (MQ=255)
                                                                                    cccAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAAcc                                              <  2:443627/140‑1 (MQ=255)
                                                                                       aaGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGAt                                           <  1:889192/140‑1 (MQ=255)
                                                                                        agacAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATc                                          >  1:955352/4‑140 (MQ=255)
                                                                                         ggCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCg                                         <  2:826947/140‑1 (MQ=255)
                                                                                          gCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAAc                                               >  2:435493/1‑133 (MQ=255)
                                                                                          gCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAAc                                               <  1:435493/133‑1 (MQ=255)
                                                                                          gCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCAAAATTTGATTGTTATCGCCAATCTGTAAAGGGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGATCGTGACAACCGATCGc                                        >  1:385908/1‑140 (MQ=255)
                                                                                            aaTATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCaa                                      >  1:938643/1‑140 (MQ=255)
                                                                                               atCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACta                                   <  2:470744/140‑1 (MQ=255)
                                                                                               atCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACta                                   <  1:259281/140‑1 (MQ=255)
                                                                                                 cgcgACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGACAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACtata                                 >  2:968879/1‑140 (MQ=255)
                                                                                                   cgACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCg                                         <  2:800852/130‑1 (MQ=255)
                                                                                                   cgACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCg                                         >  1:800852/1‑130 (MQ=255)
                                                                                                    gACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGAt                                           <  2:860067/127‑1 (MQ=255)
                                                                                                    gACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGAt                                           >  1:860067/1‑127 (MQ=255)
                                                                                                             aaTCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGc                     <  2:402263/140‑1 (MQ=255)
                                                                                                                    gACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGAtttt              <  2:270304/140‑1 (MQ=255)
                                                                                                                     aCAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTg             <  2:859953/140‑1 (MQ=255)
                                                                                                                     aCAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTg             <  2:912211/140‑1 (MQ=255)
                                                                                                                          cGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCt        >  2:891773/1‑140 (MQ=255)
                                                                                                                               ttCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGc   <  1:534759/140‑1 (MQ=255)
                                                                                                                                tCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCa  <  1:64130/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
CCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCA  >  NC_000913/1719097‑1719364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A