Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,801,794	T→C	E259E (GAA→GAG)	thrS ←	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,801,794	0	T	C	100.0%	161.4 / NA	45	E259E (GAA→GAG)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/19); total (26/19)

ACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAA  >  NC_000913/1801666‑1801928

acacGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCat                                                                                                                             <  1:650830/140‑1 (MQ=255)

acacGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCat                                                                                                                             <  1:323983/140‑1 (MQ=255)

      tCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTa                                                                                                                       >  2:557152/1‑140 (MQ=255)

         atcatGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCt                                                                                                                                 <  2:442824/127‑1 (MQ=255)

         atcatGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCt                                                                                                                                 >  1:442824/1‑127 (MQ=255)

         atcatGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAg                                                                                                                    >  1:463377/1‑140 (MQ=255)

                aCGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGc                                                                                                             >  1:8930/1‑140 (MQ=255)

                 cGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCt                                                                                                            >  2:771891/1‑140 (MQ=255)

                       tttAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCATCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAc                                                                                                      <  2:986049/140‑1 (MQ=255)

                         tAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCg                                                                                                    >  2:160130/1‑140 (MQ=255)

                              tCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGAtttt                                                                                               >  2:591992/1‑140 (MQ=255)

                                  gATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACgg                                                                                           >  2:767390/1‑140 (MQ=255)

                                        ggTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTcg                                                                                     <  1:160130/140‑1 (MQ=255)

                                         gTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTcgc                                                                                    >  1:1078429/1‑140 (MQ=255)

                                               tttCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTcg                                                                              >  1:966416/1‑140 (MQ=255)

                                                 tCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTcgcg                                                                            >  2:1027592/1‑140 (MQ=255)

                                                        aGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGcttctt                                                                     >  2:807241/1‑140 (MQ=255)

                                                         gaacgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTcgcg                                                                                   >  2:97235/1‑125 (MQ=255)

                                                         gaacgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTcgcg                                                                                   <  1:97235/125‑1 (MQ=255)

                                                             gaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAgg                                                                <  2:440371/140‑1 (MQ=255)

                                                             gaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAgg                                                                <  2:1078429/140‑1 (MQ=255)

                                                             gaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAgg                                                                <  1:1027592/140‑1 (MQ=255)

                                                             gaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAgg                                                                <  1:771891/140‑1 (MQ=255)

                                                             gaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAgg                                                                <  1:767390/140‑1 (MQ=255)

                                                                      cTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAgg                                                       <  2:841756/140‑1 (MQ=255)

                                                                        tCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTa                                                     <  1:58842/140‑1 (MQ=255)

                                                                         ccAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTaa                                                    >  1:311347/1‑140 (MQ=255)

                                                                                 cGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAg                                            >  2:994878/1‑140 (MQ=255)

                                                                                       aTGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCttt                                      >  2:400864/1‑140 (MQ=255)

                                                                                       aTGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCttt                                      >  2:1019739/1‑140 (MQ=255)

                                                                                       aTGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCttt                                      >  2:415392/1‑140 (MQ=255)

                                                                                           tCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTg                                  >  1:243513/1‑140 (MQ=255)

                                                                                                ccGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGc                             >  2:786663/1‑140 (MQ=255)

                                                                                                ccGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGc                             >  2:829806/1‑140 (MQ=255)

                                                                                                ccGTCGTTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGc                             >  2:937083/1‑140 (MQ=255)

                                                                                                     gtTGTGCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACg                        >  2:143655/1‑140 (MQ=255)

                                                                                                        gtgCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGC‑TTTTTGTCTGCCCACGccgt                    <  1:1019739/140‑1 (MQ=255)

                                                                                                         tgCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgt                    <  2:966416/140‑1 (MQ=255)

                                                                                                         tgCCAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgt                    <  1:1027882/140‑1 (MQ=255)

                                                                                                           ccAGAATACCATACCCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgtac                  >  1:444089/1‑140 (MQ=255)

                                                                                                                   ccataccCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATa          <  1:143655/140‑1 (MQ=255)

                                                                                                                   ccataccCGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATa          <  2:902575/140‑1 (MQ=255)

                                                                                                                         ccGGCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGc    <  1:197447/140‑1 (MQ=255)

                                                                                                                           ggCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCaa  >  1:1056278/1‑140 (MQ=255)

                                                                                                                           ggCGCCTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGATTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCaa  >  2:196114/1‑140 (MQ=255)

ACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAA  >  NC_000913/1801666‑1801928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A