Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,910,848	G→A	L191L (CTG→CTA)	yebQ →	putative transporter YebQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,910,848	0	G	A	97.7%	134.7 / ‑4.1	43	L191L (CTG→CTA)	yebQ	putative transporter YebQ
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (20/22); total (21/22)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

GCCGTCTCTTCTGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGTTAATTGCTGCG  >  NC_000913/1910723‑1910983

gCCGTCTCTTCTGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTtc                                                                                                                           >  1:692303/1‑140 (MQ=255)

 ccGTCTCTTCTGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTtct                                                                                                                          <  2:173516/140‑1 (MQ=255)

     ctctTCTGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTcgcg                                                                                                                      >  2:801638/1‑140 (MQ=255)

     ctctTCTGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCCCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTcgcg                                                                                                                      <  2:374034/140‑1 (MQ=255)

      tcttctGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCgcgc                                                                                                                     <  2:446046/140‑1 (MQ=255)

                         cAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAc                                                                                                  <  2:603139/140‑1 (MQ=255)

                         cAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAc                                                                                                  <  2:1061012/140‑1 (MQ=255)

                         cAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAc                                                                                                  <  2:568412/140‑1 (MQ=255)

                          aaTTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAcc                                                                                                 >  1:780401/1‑140 (MQ=255)

                                   agcaATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAgcg                                                                                        <  2:229307/140‑1 (MQ=255)

                                    gcaATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAgcgc                                                                                       >  2:349308/1‑140 (MQ=255)

                                       aTCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGt                                                                                    >  2:600216/1‑140 (MQ=255)

                                            ctcCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGtgatga                                                                               <  1:801638/140‑1 (MQ=255)

                                            ctcCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGtgatga                                                                               <  1:84921/140‑1 (MQ=255)

                                                  cGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGt                                                                         >  1:744714/1‑140 (MQ=255)

                                                    cATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTa                                                                       >  1:960389/1‑140 (MQ=255)

                                                         tGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTGAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCtt                                                                  <  2:543057/140‑1 (MQ=255)

                                                         tGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTGAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCtt                                                                  >  1:543057/1‑140 (MQ=255)

                                                         tGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCtt                                                                  <  2:718793/140‑1 (MQ=255)

                                                             aaTGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGc                                                              <  2:627306/140‑1 (MQ=255)

                                                                 gTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGAGATGAACGCGTTAACCTTCGGCCTGc                                                          >  2:962013/1‑140 (MQ=255)

                                                                     tttttAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTAt                                                      >  1:729847/1‑140 (MQ=255)

                                                                           aTCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCt                                                            >  2:39759/1‑128 (MQ=255)

                                                                           aTCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCt                                                            <  1:39759/128‑1 (MQ=255)

                                                                           aTCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGc                                                >  1:216892/1‑140 (MQ=255)

                                                                                cGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGa                                           >  1:1066050/1‑140 (MQ=255)

                                                                                cGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGa                                           <  1:379406/140‑1 (MQ=255)

                                                                                 gTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAg                                          <  2:744714/140‑1 (MQ=255)

                                                                                          ggTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACt                                                  <  2:447528/123‑1 (MQ=255)

                                                                                          ggTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACt                                                  >  1:447528/1‑123 (MQ=255)

                                                                                           gTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCt                                <  2:1066050/140‑1 (MQ=255)

                                                                                               tatCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGc                                 >  1:1056198/1‑135 (MQ=255)

                                                                                               tatCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGc                                 <  2:1056198/135‑1 (MQ=255)

                                                                                                    cccTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCaa                       >  2:816473/1‑140 (MQ=255)

                                                                                                      cTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATc                     >  2:61075/1‑140 (MQ=255)

                                                                                                        gCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGc                   <  2:109655/140‑1 (MQ=255)

                                                                                                         cTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCt                  <  1:61075/140‑1 (MQ=255)

                                                                                                          ttCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTg                 <  2:960389/140‑1 (MQ=255)

                                                                                                               gCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGtt            >  2:17390/1‑140 (MQ=255)

                                                                                                                   tGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGTTAAtt        >  2:671325/1‑140 (MQ=255)

                                                                                                                     cGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGTTAATtgc      >  1:975635/1‑140 (MQ=255)

                                                                                                                       ttttCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGTTAATtgctg    >  2:317981/1‑140 (MQ=255)

                                                                                                                         ttCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGTTAATTGCTGCg  <  1:73308/140‑1 (MQ=255)

GCCGTCTCTTCTGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGTTAATTGCTGCG  >  NC_000913/1910723‑1910983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A