Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,471,844	T→C	pseudogene (219/519 nt)	yfdL ←	CPS‑53 (KpLE1) prophage; putative uncharacterized protein YfdL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,471,844	0	T	C	100.0%	141.6 / NA	40	pseudogene (219/519 nt)	yfdL	CPS‑53 (KpLE1) prophage; putative uncharacterized protein YfdL
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/18); total (22/18)

AAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCG  >  NC_000913/2471719‑2471980

aaaTAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGcccc                                                                                                                            <  2:208166/140‑1 (MQ=255)

           tgtgTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACAt                                                                                                                 >  2:239099/1‑140 (MQ=255)

             tgtCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACAttt                                                                                                               >  2:802520/1‑140 (MQ=255)

                ccTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCtt                                                                                                            >  2:169766/1‑140 (MQ=255)

                    agaCGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAg                                                                                                        >  2:795173/1‑140 (MQ=255)

                    agaCGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAg                                                                                                        >  2:239116/1‑140 (MQ=255)

                       cGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATGTCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCg                                                                                                     >  2:489816/1‑140 (MQ=255)

                       cGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCg                                                                                                     >  1:646375/1‑140 (MQ=255)

                         tCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCt                                                                                                   >  1:230874/1‑140 (MQ=255)

                                       aTTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAg                                                                                     >  1:267221/1‑140 (MQ=255)

                                         tGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGt                                                                                   <  2:646375/140‑1 (MQ=255)

                                          ggCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGtt                                                                                  <  2:230874/140‑1 (MQ=255)

                                          ggCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGtt                                                                                  >  2:886504/1‑140 (MQ=255)

                                          ggCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGtt                                                                                  >  2:874440/1‑140 (MQ=255)

                                                cATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgag                                                                            >  2:702253/1‑140 (MQ=255)

                                                 aTTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgaga                                                                           <  2:908505/140‑1 (MQ=255)

                                                 aTTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgaga                                                                           <  1:239116/140‑1 (MQ=255)

                                                 aTTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgaga                                                                           <  1:312124/140‑1 (MQ=255)

                                                 aTTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgaga                                                                           <  1:795173/140‑1 (MQ=255)

                                                 aTTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCAGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgaga                                                                           <  2:494115/140‑1 (MQ=255)

                                                          ttAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGc                                                                  >  2:255931/1‑140 (MQ=255)

                                                                    tGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGt                                                        >  2:462101/1‑140 (MQ=255)

                                                                    tGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGt                                                        >  2:874752/1‑140 (MQ=255)

                                                                     ggCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTc                                                       <  2:267221/140‑1 (MQ=255)

                                                                       cAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCa                                                     >  1:915621/1‑140 (MQ=255)

                                                                             ttGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGa                                               <  1:874752/140‑1 (MQ=255)

                                                                              tGATGTCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGat                                              <  1:489816/140‑1 (MQ=255)

                                                                                    ccAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGc                                         <  1:544227/139‑1 (MQ=255)

                                                                                    ccAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGc                                         >  2:544227/1‑139 (MQ=255)

                                                                                             gAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGAtt                               >  1:808126/1‑140 (MQ=255)

                                                                                              aaGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTc                              >  2:153898/1‑140 (MQ=255)

                                                                                                 cAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTg                           <  1:81308/140‑1 (MQ=255)

                                                                                                   gAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCg                         >  1:439986/1‑140 (MQ=255)

                                                                                                    aaaGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCgg                        <  1:802520/140‑1 (MQ=255)

                                                                                                              aCTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAAcagca              >  2:547858/1‑140 (MQ=255)

                                                                                                                       atCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGaa     >  1:324302/1‑140 (MQ=255)

                                                                                                                       atCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGaa     <  2:808126/140‑1 (MQ=255)

                                                                                                                       atCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGaa     <  2:607085/140‑1 (MQ=255)

                                                                                                                       atCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGaa     <  1:547858/140‑1 (MQ=255)

                                                                                                                          aGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAgcg  <  1:153898/140‑1 (MQ=255)

AAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCG  >  NC_000913/2471719‑2471980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A