Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,644,370	(G)_5→6	intergenic (‑87/+63)	rlmN ← / ← ndk	23S rRNA m(2)A2503 methyltransferase/tRNA m(2)A37 methyltransferase/nucleoside diphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,644,365	1	.	G	100.0%	94.7 / NA	27	intergenic (‑82/+68)	rlmN/ndk	23S rRNA m(2)A2503 methyltransferase/tRNA m(2)A37 methyltransferase/nucleoside diphosphate kinase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (17/10); total (17/10)

TGATTTTTCCATCTTTCGTGGTGACGTTTTCAGGTGTGACTAATTGTTCAGACATATGCTATTCCGGCCTCGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCC‑GGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCG  >  NC_000913/2644229‑2644499

tGATTTTTCCATCTTTCGTGGTGACGTTTTCAGGTGTGACTAATTGTTCAGACATATGCTATTCCGGCCTCGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCC‑ggg                                                                                                                                     <  1:331852/140‑1 (MQ=255)

            cTTTCGTGGTGACGTTTTCAGGTGTGACTAATTGTTCAGACATATGCTATTCCGGCCTCGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCAtt                                                                                                                          >  2:878168/1‑140 (MQ=255)

                       aCGTTTTCAGGTGTGACTAATTGTTCAGACATATGCTATTCCGGCCTCGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCt                                                                                                               >  1:879031/1‑140 (MQ=255)

                       aCGTTTTCAGGTGTGACTAATTGTTCAGACATATGCTATTCCGGCCTCGTTATTACACGTTATGGCCCCTGGAAGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCt                                                                                                               >  1:499429/1‑140 (MQ=255)

                                         aaTTGTTCAGACATATGCTATTCCGGCCTCGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGt                                                                                             <  1:878168/140‑1 (MQ=255)

                                           ttGTTCAGACATATGCTATTCCGGCCTCGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGttt                                                                                           >  1:335691/1‑140 (MQ=255)

                                                cAGACATATGCTATTCCGGCCTCGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCAcg                                                                                      <  1:903449/140‑1 (MQ=255)

                                                        tGCTATTCCGGCCTCGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCAttt                                                                              >  2:333588/1‑140 (MQ=255)

                                                            aTTCCGGCCTCGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGa                                                                          <  2:383354/140‑1 (MQ=255)

                                                                  gCCTCGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACg                                                                           <  2:934390/133‑1 (MQ=255)

                                                                  gCCTCGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACg                                                                           >  1:934390/1‑133 (MQ=255)

                                                                      cGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAAc                                                                <  1:284656/140‑1 (MQ=255)

                                                                                 gTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCggg                                                     <  2:879031/140‑1 (MQ=255)

                                                                                 gTTATGGCCCCTGGAAGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCggg                                                     <  2:499429/140‑1 (MQ=255)

                                                                                   tATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGca                                                   >  2:542187/1‑140 (MQ=255)

                                                                                    aTGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGcac                                                  >  2:62002/1‑140 (MQ=255)

                                                                                     tGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGcaca                                                 >  1:28564/1‑140 (MQ=255)

                                                                                       gcaccTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGCACAct                                               <  2:303563/137‑1 (MQ=255)

                                                                                            tGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGCACActtcgcc                                          <  1:62002/140‑1 (MQ=255)

                                                                                                   ttGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGTGGGCACACTTCGCCTTCGCCa                                   >  1:38357/1‑140 (MQ=255)

                                                                                                   ttGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCa                                   >  2:149022/1‑140 (MQ=255)

                                                                                                   ttGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCa                                   >  1:50444/1‑140 (MQ=255)

                                                                                                   ttGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCa                                   >  1:200236/1‑140 (MQ=255)

                                                                                                     gAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCaaa                                 >  1:504904/1‑140 (MQ=255)

                                                                                                          aaGAAACGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCAAAGAAAt                            <  2:200236/140‑1 (MQ=255)

                                                                                                             aaaCGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCAAAGAAATAAg                         >  1:760884/1‑140 (MQ=255)

                                                                                                                cGCCCCGGTGAGCGGCTGCTCGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCAAAGAAATAAGCGa                      >  2:974735/1‑140 (MQ=255)

                                                                                                                                    cGTCCGGGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCg  >  2:543655/1‑140 (MQ=255)

TGATTTTTCCATCTTTCGTGGTGACGTTTTCAGGTGTGACTAATTGTTCAGACATATGCTATTCCGGCCTCGTTATTACACGTTATGGCCCCTGGAGGGTTGAAAAAAGAAACGCCCCGGTGAGCGGCTGCTCGTCC‑GGGGGCGCTGCATTGTACAAATTCTGGCGCACGGATGCCACGTTTGCACGCGGCATTTACGAAATTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCG  >  NC_000913/2644229‑2644499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A