Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,645,263	G→A	A688V (GCC→GTC)	pbpC ←	peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,645,263	0	G	A	100.0%	102.2 / NA	30	A688V (GCC→GTC)	pbpC	peptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/14); total (16/14)

GAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCGGGCAATC  >  NC_000913/2645141‑2645396

gAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGc                                                                                                                      <  2:159601/140‑1 (MQ=255)

            gTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTc                                                                                                          <  2:79936/140‑1 (MQ=255)

             ttCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCg                                                                                                         >  2:726942/1‑140 (MQ=255)

                    gCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCgg                                                                                                  <  2:341615/140‑1 (MQ=255)

                    gCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCgg                                                                                                  >  1:491148/1‑140 (MQ=255)

                     ccccTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCggt                                                                                                 >  2:477731/1‑140 (MQ=255)

                          ccACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTaa                                                                                            >  2:245908/1‑140 (MQ=255)

                          ccACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTaa                                                                                            >  1:961616/1‑140 (MQ=255)

                                  ttGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAg                                                                                    >  1:346536/1‑140 (MQ=255)

                                    gCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAgcg                                                                                  <  1:207389/140‑1 (MQ=255)

                                     cAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTaa                                                                                            <  2:83733/129‑1 (MQ=255)

                                     cAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTaa                                                                                            >  1:83733/1‑129 (MQ=255)

                                      aaCGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGcgcg                                                                                <  1:595203/140‑1 (MQ=255)

                                       aCGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGa                                                                               <  1:424194/140‑1 (MQ=255)

                                       aCGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGa                                                                               <  1:385883/140‑1 (MQ=255)

                                           cAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGtt                                                                           >  1:349961/1‑140 (MQ=255)

                                                   cTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCg                                                                   <  1:477731/140‑1 (MQ=255)

                                                     tCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCggg                                                                 <  1:888353/140‑1 (MQ=255)

                                                                 ggTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTc                                                     >  1:1006279/1‑140 (MQ=255)

                                                                 ggTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGGTc                                                     >  1:674617/1‑140 (MQ=255)

                                                                  gTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGGTcc                                                    >  2:816995/1‑140 (MQ=255)

                                                                         tttAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTa                                             >  2:274700/1‑140 (MQ=255)

                                                                             ataatCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCgg                                         >  2:1031669/1‑140 (MQ=255)

                                                                              taatCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGc                                        <  1:245908/140‑1 (MQ=255)

                                                                                atCGCGCCATCGCGAACGCCTGTCAGCTGAAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGGGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCgggct                                      <  2:77976/140‑4 (MQ=255)

                                                                                 tCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAg                                     >  2:25492/1‑140 (MQ=255)

                                                                                        aTCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTa                              <  2:489452/140‑1 (MQ=255)

                                                                                          cgcgAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATc                            <  1:726942/140‑1 (MQ=255)

                                                                                                           tGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTg           >  2:310541/1‑140 (MQ=255)

                                                                                                                  ggAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCGGGCaa    >  1:255631/1‑140 (MQ=255)

                                                                                                                    aGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCGGGCAATc  <  1:25492/140‑1 (MQ=255)

GAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCGGGCAATC  >  NC_000913/2645141‑2645396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A