Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,403,790	C→T	R103* (CGA→TGA)	yhdH →	acrylyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,403,790	0	C	T	100.0%	162.3 / NA	49	R103* (CGA→TGA)	yhdH	acrylyl‑CoA reductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/28); total (21/28)

CCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGG  >  NC_000913/3403655‑3403922

ccGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGt                                                                                                                                  <  2:376972/140‑1 (MQ=255)

      atTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAgg                                                                                                                            >  1:262969/1‑140 (MQ=255)

                tCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGActggctg                                                                                                                  >  2:852676/1‑140 (MQ=255)

                  gATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGt                                                                                                                >  1:166235/1‑140 (MQ=255)

                  gATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGt                                                                                                                >  2:839887/1‑140 (MQ=255)

                   aTTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGtt                                                                                                               >  2:717559/1‑140 (MQ=255)

                    ttttGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTg                                                                                                              <  1:839887/140‑1 (MQ=255)

                       tGCCGGAACTGTACGCCCCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCa                                                                                                           <  1:343582/140‑1 (MQ=255)

                       tGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGTGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCa                                                                                                           <  1:245835/140‑1 (MQ=255)

                            gAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCg                                                                                                      <  2:166235/140‑1 (MQ=255)

                              aCTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa                                                                                                    <  2:262969/140‑1 (MQ=255)

                              aCTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa                                                                                                    <  1:403607/140‑1 (MQ=255)

                              aCTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa                                                                                                    <  2:31532/140‑1 (MQ=255)

                                 gTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAgg                                                                                                 >  1:968199/1‑140 (MQ=255)

                                  tACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAggg                                                                                                >  1:833948/1‑140 (MQ=255)

                                       aCCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGa                                                                                           >  1:223698/1‑140 (MQ=255)

                                            cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCg                                                                                                      >  2:794693/1‑124 (MQ=255)

                                            cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCg                                                                                                      <  1:794693/124‑1 (MQ=255)

                                            cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAcgcgc                                                                                      >  1:28886/1‑140 (MQ=255)

                                            cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAc                                                                                          >  1:71781/1‑136 (MQ=255)

                                            cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAc                                                                                          <  2:71781/136‑1 (MQ=255)

                                               aGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTa                                                                                   <  1:954355/140‑1 (MQ=255)

                                                    cgcgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCa                                                                              <  1:504493/140‑1 (MQ=255)

                                                     gcgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCaa                                                                             <  2:223698/140‑1 (MQ=255)

                                                      cgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAAt                                                                            >  1:225607/1‑140 (MQ=255)

                                                       gTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATg                                                                           <  2:750232/140‑1 (MQ=255)

                                                            cATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTAt                                                                      >  1:1019760/1‑140 (MQ=255)

                                                             aTGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATc                                                                     >  2:123679/1‑140 (MQ=255)

                                                               gCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCgg                                                                   <  1:852676/140‑1 (MQ=255)

                                                                 cGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTa                                                                 <  1:717559/140‑1 (MQ=255)

                                                                      aggaggTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGcc                                                            <  1:123679/140‑1 (MQ=255)

                                                                            tgtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCg                                                                    <  2:829796/126‑1 (MQ=255)

                                                                            tgtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCg                                                                    >  1:829796/1‑126 (MQ=255)

                                                                                 cTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGc                                                 <  2:28886/140‑1 (MQ=255)

                                                                                 cTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGc                                                 <  2:419899/140‑1 (MQ=255)

                                                                                  tCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGcc                                                <  2:225607/140‑1 (MQ=255)

                                                                                     ctggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATg                                             <  2:1019760/140‑1 (MQ=255)

                                                                                     ctggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATg                                             <  1:462127/140‑1 (MQ=255)

                                                                                     ctggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCAATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATg                                             <  2:614947/140‑1 (MQ=255)

                                                                                        gctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGttt                                                       <  2:965824/127‑1 (MQ=255)

                                                                                        gctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGttt                                                       >  1:965824/1‑127 (MQ=255)

                                                                                            gggCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCtgtgtg                                      >  1:8592/1‑140 (MQ=255)

                                                                                                  tGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATgg                                >  2:940851/1‑140 (MQ=255)

                                                                                                    gTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGgcg                              <  1:346442/140‑1 (MQ=255)

                                                                                                       aaaaCCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTg                           >  2:437744/1‑140 (MQ=255)

                                                                                                                 gggTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGATGTGTGTGATGGCGCTGGAAGATGCCg                 >  2:389328/1‑140 (MQ=255)

                                                                                                                             ggAGCAGGCGTGAGTAAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGAAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGc     <  1:940851/140‑1 (MQ=255)

                                                                                                                                 cAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAgg  <  2:209936/139‑1 (MQ=255)

                                                                                                                                 cAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAgg  >  1:209936/1‑139 (MQ=255)

CCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGG  >  NC_000913/3403655‑3403922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A