Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	116	98.7	3298914	99.2%	3272522	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,430,008	C→T	A320A (GCC→GCT)	stfR →	Rac prophage; putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,430,008	0	C	T	100.0%	144.9 / NA	44	A320A (GCC→GCT)	stfR	Rac prophage; putative membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/23); total (21/23)

ACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGCGAAGACATCCGAAACGAACGCG  >  NC_000913/1429883‑1430143

aCGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGaaa                                                                                                                           <  1:1641153/140‑1 (MQ=255)

    aCGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCg                                                                                                                       >  2:1583680/1‑140 (MQ=255)

     cGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCgg                                                                                                                      >  1:1200526/1‑140 (MQ=255)

     cGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCgg                                                                                                                      >  2:551912/1‑140 (MQ=255)

             cttctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAgc                                                                                                              >  1:1600226/1‑140 (MQ=255)

                ctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGcgcc                                                                                                           >  1:1324742/1‑140 (MQ=255)

                ctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGcgcc                                                                                                           >  2:1605065/1‑140 (MQ=255)

                ctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGcgcc                                                                                                           >  1:438564/1‑140 (MQ=255)

                     aCGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATc                                                                                                      >  2:987953/1‑140 (MQ=255)

                      cGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCg                                                                                                     >  1:985204/1‑140 (MQ=255)

                       ggCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGt                                                                                                    <  2:1297944/140‑1 (MQ=255)

                        gCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTc                                                                                                   <  2:1404691/140‑1 (MQ=255)

                              ggACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTGCCGCCGGCGGAAAATGGGCAGAAAGCGCCGCATCGTCTGCGTc                                                                                             >  2:1143399/1‑140 (MQ=255)

                              ggACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTc                                                                                             <  2:1324742/140‑1 (MQ=255)

                                  agagCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACa                                                                                         >  1:429520/1‑140 (MQ=255)

                                        ccTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCcaca                                                                                   >  2:667626/1‑140 (MQ=255)

                                         cTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAAACGGCAGAAAGCGCCGCATCGACTGCTTCAACAGCCACaa                                                                                  >  2:487039/1‑140 (MQ=255)

                                                gCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGc                                                                           >  2:1328967/1‑140 (MQ=255)

                                                      ggCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAGGGGCCGGGCGGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGa                                                                     <  2:1457156/140‑1 (MQ=255)

                                                      ggCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGa                                                                     <  1:1304222/140‑1 (MQ=255)

                                                      ggCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGa                                                                     <  2:1200526/140‑1 (MQ=255)

                                                      ggCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCACAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGTGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGa                                                                     <  1:1143399/140‑1 (MQ=255)

                                                           aaaaaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCTGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGAAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCa                                                                <  1:206123/140‑1 (MQ=255)

                                                           aaaaaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCa                                                                <  2:1600226/140‑1 (MQ=255)

                                                           aaaaaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCa                                                                <  1:1328967/140‑1 (MQ=255)

                                                           aaaaaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCa                                                                <  2:1489618/140‑1 (MQ=255)

                                                           aaaaaCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCa                                                                <  2:1356753/140‑1 (MQ=255)

                                                                cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGaa                                                           <  2:1265408/140‑1 (MQ=255)

                                                                cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGaa                                                           <  2:1375069/140‑1 (MQ=255)

                                                                cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGaa                                                           <  1:1505413/140‑1 (MQ=255)

                                                                cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGaa                                                           <  1:551912/140‑1 (MQ=255)

                                                                   cGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAg                                                        >  1:917897/1‑140 (MQ=255)

                                                                     gCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGc                                                      <  1:987953/140‑1 (MQ=255)

                                                                     gCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGc                                                      <  2:985204/140‑1 (MQ=255)

                                                                      cTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGcc                                                     >  1:414836/1‑140 (MQ=255)

                                                                       ggCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCCCAGCCAGTGCTACCGCCGCGGGAAAATCGGCAGAAAGCCCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCa                                                    <  2:1621071/139‑1 (MQ=255)

                                                                            cgtcTGCCAGTGCAGCGTCAACAAGTGCGGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCgca                                               <  2:1452001/140‑1 (MQ=255)

                                                                                  ccAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCg                                         >  1:1175831/1‑140 (MQ=255)

                                                                                     gTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAgg                                      <  1:1583680/140‑1 (MQ=255)

                                                                                          gCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGc                                 >  1:1426020/1‑140 (MQ=255)

                                                                                             tCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTc                              >  1:1545003/1‑140 (MQ=255)

                                                                                             tCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTc                              >  2:736474/1‑140 (MQ=255)

                                                                                             tCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTc                              >  2:780449/1‑140 (MQ=255)

                                                                                              caacaaGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTcc                             <  2:1403596/140‑1 (MQ=255)

                                                                                                 caaGTGCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCa                          <  1:264359/140‑1 (MQ=255)

                                                                                                      gCCGGGCAGGCCTCAGCCAGTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGCGaa                     <  1:424683/140‑1 (MQ=255)

                                                                                                                         gTGCTACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGCGAAGACATCCGAAACGAAcgcg  >  1:1626130/1‑140 (MQ=255)

ACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCAGCGAAGACATCCGAAACGAACGCG  >  NC_000913/1429883‑1430143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A