Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	116	98.7	3298914	99.2%	3272522	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,439,814	T→C	D324G (GAC→GGC)	ydbK ←	putative pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,439,814	0	T	C	98.7%	284.8 / ‑4.6	78	D324G (GAC→GGC)	ydbK	putative pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (43/34); total (43/35)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

CTTTGGATGAAAGACCATAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGTCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAACCACTTCTTCACAG  >  NC_000913/1439677‑1439951

cTTTGGATGAAAGACCATAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGTcc                                                                                                                                         <  1:1078945/140‑1 (MQ=255)

         aaaGACCATAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGcc                                                                                                                                <  1:702872/140‑1 (MQ=255)

           aGACCATAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCAc                                                                                                                              <  2:835170/140‑1 (MQ=255)

            gACCATAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACg                                                                                                                             <  2:1253966/140‑1 (MQ=255)

            gACCATAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACg                                                                                                                             <  2:1009383/140‑1 (MQ=255)

            gACCATAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACg                                                                                                                             <  1:399996/140‑1 (MQ=255)

            gACCATAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACg                                                                                                                             <  1:1435611/140‑1 (MQ=255)

            gACCATAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACg                                                                                                                             <  1:100153/140‑1 (MQ=255)

              ccATAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCt                                                                                                                           <  1:758822/140‑1 (MQ=255)

              ccATAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCt                                                                                                                           <  2:1378355/140‑1 (MQ=255)

                aTAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGc                                                                                                                         >  2:1616881/1‑140 (MQ=255)

                   gcgcCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTa                                                                                                                      <  2:1366060/140‑1 (MQ=255)

                    cgcCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTAc                                                                                                                     >  2:241934/1‑140 (MQ=255)

                     gcCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACg                                                                                                                    <  2:701212/140‑1 (MQ=255)

                     cccccccAATGACACGGGGCGGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACg                                                                                                                    <  2:1067550/139‑1 (MQ=255)

                      cccACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACgg                                                                                                                   >  1:1228047/1‑140 (MQ=255)

                         accaATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATc                                                                                                                >  1:1117961/1‑140 (MQ=255)

                          ccaATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTACCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATcc                                                                                                               <  2:1485382/140‑1 (MQ=255)

                                   cGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGc                                                                                                      >  1:674104/1‑140 (MQ=255)

                                    ggggCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCt                                                                                                     <  2:1551594/140‑1 (MQ=255)

                                     gggCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCtt                                                                                                    >  1:1450980/1‑140 (MQ=255)

                                     gggCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCtt                                                                                                    >  1:1540547/1‑140 (MQ=255)

                                      ggCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTg                                                                                                   >  2:425468/1‑140 (MQ=255)

                                      ggCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTg                                                                                                   <  1:1219951/140‑1 (MQ=255)

                                       gCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGc                                                                                                  >  1:961770/1‑140 (MQ=255)

                                         agagTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAg                                                                                                >  1:955299/1‑140 (MQ=255)

                                          gagTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGt                                                                                               <  1:1319243/140‑1 (MQ=255)

                                               tCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATg                                                                                          >  2:438936/1‑140 (MQ=255)

                                                cgcgCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGt                                                                                         >  2:217844/1‑140 (MQ=255)

                                                cgcgCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGt                                                                                         >  1:564959/1‑140 (MQ=255)

                                                cgcgCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGt                                                                                         >  1:786769/1‑140 (MQ=255)

                                                cgcgCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGCTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGt                                                                                         >  2:847175/1‑140 (MQ=255)

                                                cgcgCTCACCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACACTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGt                                                                                         >  2:1151912/1‑140 (MQ=255)

                                                          attattAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGgaga                                                                               <  1:339315/140‑1 (MQ=255)

                                                                   ggCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGt                                                                      >  1:1304894/1‑140 (MQ=255)

                                                                    gCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTa                                                                     >  1:1391671/1‑140 (MQ=255)

                                                                    gCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTa                                                                     <  1:13235/140‑1 (MQ=255)

                                                                     cTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTAc                                                                    >  2:1101278/1‑140 (MQ=255)

                                                                      ttCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCg                                                                                   >  2:919291/1‑124 (MQ=255)

                                                                      ttCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCg                                                                                   <  1:919291/124‑1 (MQ=255)

                                                                      ttCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACa                                                                   >  2:1459886/1‑140 (MQ=255)

                                                                        cTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAgg                                                                 >  2:15893/1‑140 (MQ=255)

                                                                        cTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAgg                                                                 >  1:642458/1‑140 (MQ=255)

                                                                        cTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAgg                                                                 >  1:640508/1‑140 (MQ=255)

                                                                        cTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAgg                                                                 >  1:257800/1‑140 (MQ=255)

                                                                        cTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAgg                                                                 >  1:1181851/1‑140 (MQ=255)

                                                                         tGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGc                                                                >  1:645403/1‑140 (MQ=255)

                                                                          gCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCg                                                               <  1:1153264/140‑1 (MQ=255)

                                                                          gCCAGGGCGGTCATTCCATCCAGATAGGGCGGTTCTGCTTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCg                                                               <  1:1280768/140‑1 (MQ=255)

                                                                                gCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACttt                                                         >  2:999193/1‑140 (MQ=255)

                                                                                            tCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAAc                                             >  2:318796/1‑140 (MQ=255)

                                                                                              cAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACtt                                           >  1:200800/1‑140 (MQ=255)

                                                                                              cAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACtt                                           >  1:1196690/1‑140 (MQ=255)

                                                                                                gATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACtttt                                         >  2:1588098/1‑140 (MQ=255)

                                                                                                gATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACtttt                                         >  1:744784/1‑140 (MQ=255)

                                                                                                 aTAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACttttt                                        >  1:37065/1‑140 (MQ=255)

                                                                                                     agCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGcc                                    >  2:1158087/1‑140 (MQ=255)

                                                                                                      gCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCa                                   >  2:328711/1‑140 (MQ=255)

                                                                                                        ggTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACg                                 <  1:242962/140‑1 (MQ=255)

                                                                                                               ccTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGc                          <  1:425468/140‑1 (MQ=255)

                                                                                                                cTGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCa                         <  2:1391671/140‑1 (MQ=255)

                                                                                                                 tGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCaa                        <  1:586809/140‑1 (MQ=255)

                                                                                                                 tGGGCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCaa                        <  2:955299/140‑1 (MQ=255)

                                                                                                                   ggCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAAtt                      >  1:134531/1‑140 (MQ=255)

                                                                                                                    gCACCGGGTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATtc                     >  1:820660/1‑140 (MQ=255)

                                                                                                                          ggTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAAc               <  2:640508/140‑1 (MQ=255)

                                                                                                                          ggTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAAc               <  2:257800/140‑1 (MQ=255)

                                                                                                                          ggTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAAc               >  2:495545/1‑140 (MQ=255)

                                                                                                                          ggTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAAc               <  1:217844/140‑1 (MQ=255)

                                                                                                                          ggTTCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAAc               <  1:241934/140‑1 (MQ=255)

                                                                                                                            ttCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAACCa             <  1:1416327/140‑1 (MQ=255)

                                                                                                                             tCTTTGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAACCAc            <  2:744784/140‑1 (MQ=255)

                                                                                                                                 tGGTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAACCActtct        <  2:1228047/140‑1 (MQ=255)

                                                                                                                                  ggTTCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAACCActtctt       >  2:1546039/1‑140 (MQ=255)

                                                                                                                                     tCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGTGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAACCACTTCTTcac    >  1:215370/1‑140 (MQ=255)

                                                                                                                                     tCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAACCACTTCTTcac    <  2:571789/140‑1 (MQ=255)

                                                                                                                                     tCTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAACCACTTCTTcac    <  2:1139000/140‑1 (MQ=255)

                                                                                                                                      cTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAACCACTTCTTcaca   >  1:1102270/1‑140 (MQ=255)

                                                                                                                                      cTGCCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAA‑GGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAACCACTTCTTCACAg  >  2:1070990/1‑140 (MQ=255)

CTTTGGATGAAAGACCATAGCGCCCACCAATGACACGGGGCAGAGTTTCGCGCTCGCCATTATTAAAGGCTTCTGCCAGTGCGGTCATTACATCCAGATAGAGCGGTTCTGCCTGGGCACCGGGTTCTTTGGTTCTGTCCAGTACCGCCACGCTGCGTACGGATCCCGGCAGAGCTTGCAGTAAATGTTTAGCGGAGAAGGGGCGGTACAGGCGAACTTTCAGCACGCCAACTTTTTCGCCACGGGTTAGCAATTCATCAACCACTTCTTCACAG  >  NC_000913/1439677‑1439951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A