Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,440,941	(A)_7→8	intergenic (‑157/‑117)	ydbK ← / → ydbJ	putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,440,934	1	.	A	100.0%	153.3 / NA	44	intergenic (‑150/‑124)	ydbK/ydbJ	putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (19/25); total (19/25)

CATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑AAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGAGCAGC  >  NC_000913/1440797‑1441068

cATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑aa                                                                                                                                      <  1:1021592/140‑1 (MQ=255)

     cgcAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑aaaaaaa                                                                                                                                 >  2:209994/1‑140 (MQ=255)

     cgcAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑aaaaaaa                                                                                                                                 >  2:1125990/1‑140 (MQ=255)

     cgcAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑a                                                                                                                                       >  2:43194/1‑134 (MQ=255)

     cgcAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑a                                                                                                                                       <  1:43194/134‑1 (MQ=255)

               gggCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATaa                                                                                                                        >  1:1052637/1‑140 (MQ=255)

                    cACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAg                                                                                                                   >  1:1019651/1‑140 (MQ=255)

                                   cgcCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATg                                                                                                           >  1:10099/1‑133 (MQ=255)

                                   cgcCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATg                                                                                                           <  2:10099/133‑1 (MQ=255)

                                   cgcCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCa                                                                                                    <  1:209994/140‑1 (MQ=255)

                                   cgcCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCa                                                                                                    >  1:713834/1‑140 (MQ=255)

                                        agtagtAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAAcgcg                                                                                               <  2:181458/140‑1 (MQ=255)

                                           agtaATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAg                                                                                            <  2:969780/140‑1 (MQ=255)

                                               aTCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACa                                                                                        <  2:374730/140‑1 (MQ=255)

                                                tCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGAAAAAATGCGGGGCAACGCGCAGGACAg                                                                                       >  1:586713/1‑140 (MQ=255)

                                                    tCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGctct                                                                                   >  2:611314/1‑140 (MQ=255)

                                                    tCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGctct                                                                                   >  1:67915/1‑140 (MQ=255)

                                                    tCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGctct                                                                                   >  1:1087568/1‑140 (MQ=255)

                                                    tCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGctct                                                                                   >  2:1159368/1‑140 (MQ=255)

                                                    tCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGctct                                                                                   >  2:855413/1‑140 (MQ=255)

                                                    tCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGctct                                                                                   <  1:20428/140‑1 (MQ=255)

                                                       aTTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGa                                                                                >  1:1129913/1‑140 (MQ=255)

                                                         tttAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGAACAGCTCTCGAcg                                                                              <  2:636048/140‑1 (MQ=255)

                                                           tAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGAcgcc                                                                            <  2:586713/140‑1 (MQ=255)

                                                           tAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGAcgcc                                                                            <  2:1056043/140‑1 (MQ=255)

                                                              cAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACgccgcc                                                                         <  2:1019651/140‑1 (MQ=255)

                                                               aaaTGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGccc                                                                        <  1:856273/140‑1 (MQ=255)

                                                                aaTGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCct                                                                       <  1:953045/140‑1 (MQ=255)

                                                                  tGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCctct                                                                     >  2:51229/1‑140 (MQ=255)

                                                                     cTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCtgtg                                                                  <  1:51229/140‑1 (MQ=255)

                                                                       ttcttcTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGtgct                                                                <  2:67915/140‑1 (MQ=255)

                                                                         cttctGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGtgctgc                                                              <  2:1129913/140‑1 (MQ=255)

                                                                         cttctGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGtgctgc                                                              <  1:855413/140‑1 (MQ=255)

                                                                           tctGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCt                                                            >  2:812498/1‑140 (MQ=255)

                                                                            ctGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCta                                                           <  2:439410/140‑1 (MQ=255)

                                                                              gCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCtatt                                                         <  2:1058691/140‑1 (MQ=255)

                                                                                         tttGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCgg                                              >  1:946856/1‑140 (MQ=255)

                                                                                                cccccACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGtt                                       >  2:949076/1‑140 (MQ=255)

                                                                                                cccccACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGtt                                       <  2:49282/140‑1 (MQ=255)

                                                                                                      caTCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCtt                                 >  2:266682/1‑140 (MQ=255)

                                                                                                      caTCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCtt                                 >  2:1210119/1‑140 (MQ=255)

                                                                                                       aTCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTg                                <  1:1210119/140‑1 (MQ=255)

                                                                                                       aTCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTg                                <  2:1052637/140‑1 (MQ=255)

                                                                                                                  gaatgTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAgg                     <  1:360629/140‑1 (MQ=255)

                                                                                                                         ttGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGaaaa              >  1:331208/1‑140 (MQ=255)

                                                                                                                                aaCAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGa       <  2:946856/140‑1 (MQ=255)

                                                                                                                                 aCAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAGTGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGag      >  1:1082263/1‑140 (MQ=255)

                                                                                                                                   agagGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGagca    <  2:331208/140‑1 (MQ=255)

                                                                                                                                     agGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGagcagc  <  2:55336/140‑1 (MQ=255)

                                                                                                                                     agGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGagcagc  <  1:31469/140‑1 (MQ=255)

CATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑AAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGAGCAGC  >  NC_000913/1440797‑1441068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A