Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,441,545	A→G	C67R (TGT→CGT)	hslJ ←	lipoprotein implicated in Novobiocin resistance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,441,545	0	A	G	100.0%	208.4 / NA	56	C67R (TGT→CGT)	hslJ	lipoprotein implicated in Novobiocin resistance
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (32/24); total (32/24)

CCTTCTTTCAGCATTTCGCTAATGGTGTTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACACATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACA  >  NC_000913/1441409‑1441672

ccTTCTTTCAGCATTTCGCTAATGGTGTTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCAt                                                                                                                              >  1:946821/1‑140 (MQ=255)

 cttcttTCAGCATTTCGCTAATGGTGTTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCAtg                                                                                                                             >  2:509723/1‑140 (MQ=255)

              ttCGCTAATGGTGTTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAatca                                                                                                                >  1:179826/1‑140 (MQ=255)

                     aTGGTGTTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCAtttt                                                                                                         <  1:36211/140‑1 (MQ=255)

                        gtgtTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTc                                                                                                      >  2:268009/1‑140 (MQ=255)

                        gtgtTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTc                                                                                                      >  2:509727/1‑140 (MQ=255)

                         tgtTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCa                                                                                                     <  2:179826/140‑1 (MQ=255)

                          gtTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCAc                                                                                                    <  1:964432/140‑1 (MQ=255)

                               cGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAg                                                                                               >  2:632809/1‑140 (MQ=255)

                                   ttCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGa                                                                                           >  2:438634/1‑140 (MQ=255)

                                             tGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCgg                                                                                 >  2:1014713/1‑140 (MQ=255)

                                               cGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGAt                                                                               >  1:729968/1‑140 (MQ=255)

                                               cGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGAt                                                                               >  1:1105223/1‑140 (MQ=255)

                                                     aGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTAt                                                                         <  1:632809/140‑1 (MQ=255)

                                                       cgcACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCg                                                                       <  2:729968/140‑1 (MQ=255)

                                                            atcatACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCACCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGt                                                                  <  2:546778/140‑1 (MQ=255)

                                                              catACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCa                                                                <  1:268009/140‑1 (MQ=255)

                                                                tACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACg                                                              <  2:946821/140‑1 (MQ=255)

                                                                     gTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCtt                                                         <  1:509727/140‑1 (MQ=255)

                                                                      tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGt                                                                  >  2:697678/1‑130 (MQ=255)

                                                                      tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGt                                                                  <  1:697678/130‑1 (MQ=255)

                                                                      tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTa                                                        >  1:886533/1‑140 (MQ=255)

                                                                      tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTa                                                        >  2:552769/1‑140 (MQ=255)

                                                                      tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTa                                                        >  2:104805/1‑140 (MQ=255)

                                                                      tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTa                                                        >  2:1069560/1‑140 (MQ=255)

                                                                      tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTa                                                        >  2:942669/1‑140 (MQ=255)

                                                                        aTTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTAcc                                                      >  2:935399/1‑140 (MQ=255)

                                                                          tGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGt                                                    >  2:899095/1‑140 (MQ=255)

                                                                               gCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACg                                               <  1:88062/140‑1 (MQ=255)

                                                                               gCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACg                                               <  1:509723/140‑1 (MQ=255)

                                                                               gCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACg                                               <  1:552769/140‑1 (MQ=255)

                                                                               gCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACg                                               >  1:1028441/1‑140 (MQ=255)

                                                                               gCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCAATTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACg                                               <  1:1015419/140‑1 (MQ=255)

                                                                                    ttGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTc                                          >  2:386997/1‑140 (MQ=255)

                                                                                     tGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTcc                                         >  1:1198007/1‑140 (MQ=255)

                                                                                          gTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGcac                                    <  2:886533/140‑1 (MQ=255)

                                                                                           tCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGcaca                                   >  1:947286/1‑140 (MQ=255)

                                                                                             aGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACaaa                                 >  2:1197317/1‑140 (MQ=255)

                                                                                              gTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAg                                <  1:1014713/140‑1 (MQ=255)

                                                                                               ttCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGc                               >  1:833660/1‑140 (MQ=255)

                                                                                                 cACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCga                             >  2:296340/1‑140 (MQ=255)

                                                                                                 cACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCga                             <  1:942669/140‑1 (MQ=255)

                                                                                                  aCCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCgat                            >  2:401832/1‑140 (MQ=255)

                                                                                                            aGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAg                  <  2:1105223/140‑1 (MQ=255)

                                                                                                            aGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAg                  <  1:899095/140‑1 (MQ=255)

                                                                                                            aGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCATTCCAGCACAAAGCGATGATGCTGTAg                  <  1:935399/140‑1 (MQ=255)

                                                                                                              tttGCCTTCACCGCTAAAGCGGTTACGCATGCTGCTGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCt                >  1:501984/1‑140 (MQ=255)

                                                                                                              tttGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCg                              <  2:979101/126‑1 (MQ=255)

                                                                                                              tttGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCg                              >  1:979101/1‑126 (MQ=255)

                                                                                                              tttGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCt                >  2:1186222/1‑140 (MQ=255)

                                                                                                                tGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGt              >  2:69928/1‑140 (MQ=255)

                                                                                                                 gCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTATGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGtt             <  1:386997/140‑1 (MQ=255)

                                                                                                                  ccTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTc            <  2:947286/140‑1 (MQ=255)

                                                                                                                        ccGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGt      >  2:947760/1‑140 (MQ=255)

                                                                                                                            tAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACa  >  2:846328/1‑140 (MQ=255)

                                                                                                                            tAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACa  <  1:104805/140‑1 (MQ=255)

                                                                                                                            tAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACa  >  2:372638/1‑140 (MQ=255)

CCTTCTTTCAGCATTTCGCTAATGGTGTTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACACATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACA  >  NC_000913/1441409‑1441672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A