Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,035,711	A→G	T216A (ACC→GCC)	hyaC →	hydrogenase 1 cytochrome b subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,035,711	0	A	G	100.0%	204.6 / NA	57	T216A (ACC→GCC)	hyaC	hydrogenase 1 cytochrome b subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/31); total (26/31)

TCTACTGGACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGG  >  NC_000913/1035574‑1035845

tCTACTGGACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTccgcc                                                                                                                                      <  2:348533/140‑1 (MQ=255)

       gACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCa                                                                                                                               <  2:59400/140‑1 (MQ=255)

       gACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCa                                                                                                                               >  1:85440/1‑140 (MQ=255)

           ggTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACgg                                                                                                                           <  1:279187/140‑1 (MQ=255)

           ggTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACgg                                                                                                                           <  1:754602/140‑1 (MQ=255)

            gTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCAGCCATGGTCAACGGc                                                                                                                          >  2:46668/1‑140 (MQ=255)

               gCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTAc                                                                                                                       >  2:816410/1‑140 (MQ=255)

                cAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTAcc                                                                                                                      <  1:569531/140‑1 (MQ=255)

                 aaCTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCg                                                                                                                     >  1:167268/1‑140 (MQ=255)

                      aaTGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGcc                                                                                                                >  2:743172/1‑140 (MQ=255)

                             aTTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCT‑CGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAAttt                                                                                                        >  2:70910/1‑140 (MQ=255)

                               tCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTGGCCACAAATTTg                                                                                                       >  2:841972/1‑140 (MQ=255)

                                  caGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCa                                                                                                    <  2:395020/140‑1 (MQ=255)

                                    gCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCaaa                                                                                                  >  2:96959/1‑140 (MQ=255)

                                    gCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCaaa                                                                                                  <  1:79147/140‑1 (MQ=255)

                                      tGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTGGCCACAAATTTGGCAAAAt                                                                                                <  1:841972/140‑1 (MQ=255)

                                       ggCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATa                                                                                               <  2:85440/140‑1 (MQ=255)

                                         cATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTgg                                                                                                      >  1:121921/1‑131 (MQ=255)

                                         cATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTgg                                                                                                      <  2:121921/131‑1 (MQ=255)

                                          aTCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGt                                                                                            <  2:167268/140‑1 (MQ=255)

                                                  gggATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGa                                                                                    >  1:320011/1‑140 (MQ=255)

                                                    gATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGc                                                                                  <  2:19965/140‑1 (MQ=255)

                                                        tgGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTc                                                                              >  2:268547/1‑140 (MQ=255)

                                                        tgGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTc                                                                              >  2:347649/1‑140 (MQ=255)

                                                         ggCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGtt                                                                               >  2:28842/1‑138 (MQ=255)

                                                         ggCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGtt                                                                               <  1:28842/138‑1 (MQ=255)

                                                         ggCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCa                                                                             >  1:149371/1‑140 (MQ=255)

                                                         ggCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCa                                                                             <  1:780572/140‑1 (MQ=255)

                                                         ggCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCa                                                                             <  1:347649/140‑1 (MQ=255)

                                                            tGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATga                                                                          <  1:361636/140‑1 (MQ=255)

                                                               ttGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATgagcg                                                                       >  1:630517/1‑140 (MQ=255)

                                                                 ggCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCAGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATgagcgag                                                                     <  1:46668/140‑1 (MQ=255)

                                                                 ggCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATATCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATgagcgag                                                                     <  1:41264/140‑1 (MQ=255)

                                                                        ttGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAAcgcg                                                              <  1:743172/140‑1 (MQ=255)

                                                                          gtgATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCgtg                                                            <  2:320011/140‑1 (MQ=255)

                                                                            gATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCgtggt                                                          <  1:816410/140‑1 (MQ=255)

                                                                            gATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCgtggt                                                          <  1:102473/140‑1 (MQ=255)

                                                                                 gTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCa                                                     <  1:96959/140‑1 (MQ=255)

                                                                                  tCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCAt                                                    >  1:598128/1‑140 (MQ=255)

                                                                                     tGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATggg                                                 <  1:498487/140‑1 (MQ=255)

                                                                                     tGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATggg                                                 >  1:146063/1‑140 (MQ=255)

                                                                                          aCATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTgg                                            >  2:262351/1‑140 (MQ=255)

                                                                                           cATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTggg                                           <  1:143748/140‑1 (MQ=255)

                                                                                           cATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTggg                                           <  1:535072/140‑1 (MQ=255)

                                                                                           cATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTggg                                           >  2:535072/1‑140 (MQ=255)

                                                                                            aTGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCt                                              <  2:25382/136‑1 (MQ=255)

                                                                                            aTGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCt                                              >  1:25382/1‑136 (MQ=255)

                                                                                            aTGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGc                                          >  1:512544/1‑140 (MQ=255)

                                                                                                cgcTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAAcc                                      >  1:206273/1‑140 (MQ=255)

                                                                                                cgcTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAAcc                                      >  2:703058/1‑140 (MQ=255)

                                                                                                cgcTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAAcc                                      >  2:407398/1‑140 (MQ=255)

                                                                                                cgcTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAAcc                                      >  1:739650/1‑140 (MQ=255)

                                                                                                         aaGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTggg                             <  2:72896/140‑1 (MQ=255)

                                                                                                         aaGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTggg                             <  2:146063/140‑1 (MQ=255)

                                                                                                          aGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGc                            <  1:70910/140‑1 (MQ=255)

                                                                                                                      cgacgaCACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCtt                <  2:130915/140‑1 (MQ=255)

                                                                                                                      cgacgaCACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCtt                >  2:925561/1‑140 (MQ=255)

                                                                                                                                    tctcCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTgg  <  2:667197/140‑1 (MQ=255)

TCTACTGGACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGG  >  NC_000913/1035574‑1035845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A