Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,060,354	A→G	T367A (ACG→GCG)	torA →	trimethylamine N‑oxide reductase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,060,354	0	A	G	100.0%	113.7 / NA	32	T367A (ACG→GCG)	torA	trimethylamine N‑oxide reductase 1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/16); total (16/16)

TGGGTTTTGAGCAGTTCCTGCCGTATCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAACGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTGGCGGCGATGCTGGGGCAAATTGGCCTGCCAGGTGGTGGTTTTGGTTTTGGC  >  NC_000913/1060217‑1060479

tgGGTTTTGAGCAGTTCCTGCCGTATCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCg                                                                                                                             >  1:764838/1‑140 (MQ=255)

tgGGTTTTGAGCAGTTCCTGCCGTATCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCg                                                                                                                             >  1:451135/1‑140 (MQ=255)

     tttGAGCAGTTCCTGCCGTATCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCg                                                                                                                             >  1:53853/1‑135 (MQ=255)

            aGTTCCTGCCGTATCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTgct                                                                                                                 <  1:122455/140‑1 (MQ=255)

              ttCCTGCCGTATCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTgctgg                                                                                                               >  2:4626/1‑140 (MQ=255)

              ttCCTGCCGTATCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTgctgg                                                                                                               >  1:834741/1‑140 (MQ=255)

                        aTCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTgctggc                                                                                                              <  1:596658/131‑1 (MQ=255)

                        aTCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTgctggc                                                                                                              >  2:596658/1‑131 (MQ=255)

                        aTCTGCTGGGTGAGAAACACGATCACCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGACGCCGAAACCATCCGTGCCCTGGCACGGCCGCGGGCGGCGAACAGAGAGCAGATTTTTGCTGGGTGgggcgtg                                                                                                     >  2:651577/1‑140 (MQ=255)

                         tCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGgtgcgtgc                                                                                                    <  1:215294/140‑1 (MQ=255)

                            gctgGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGc                                                                                                 <  1:510322/140‑1 (MQ=255)

                              tgGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGt                                                                                               <  2:51284/140‑1 (MQ=255)

                                 gTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATg                                                                                            >  1:552028/1‑140 (MQ=255)

                                   gagaAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATgca                                                                                          <  2:451135/140‑1 (MQ=255)

                                   gagaAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATgca                                                                                          <  2:764838/140‑1 (MQ=255)

                                     gaAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATgcagc                                                                                        >  1:841961/1‑140 (MQ=255)

                                       aaGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCAc                                                                                      <  2:552028/140‑1 (MQ=255)

                                           cGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCTGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTg                                                                                  <  2:834741/140‑1 (MQ=255)

                                           cGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTg                                                                                  <  1:4626/140‑1 (MQ=255)

                                           cGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTg                                                                                  >  1:570724/1‑140 (MQ=255)

                                              tCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAAc                                                                               >  2:549327/1‑140 (MQ=255)

                                                                aTGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATtg                                                             <  2:841961/140‑1 (MQ=255)

                                                                    gCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGt                                                         >  1:925667/1‑140 (MQ=255)

                                                                            aCTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTggcgg                                                 <  2:925667/140‑1 (MQ=255)

                                                                             cTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTggcggc                                                >  2:643483/1‑140 (MQ=255)

                                                                               gACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGTGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCAGGGTGAACGGTGGGCGTGGATGATTGTGGTTCTGGGGGCGa                                              >  2:838917/1‑140 (MQ=255)

                                                                                                aaaCCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTGGCGGCGATGCTGGGGCAAATTGGc                             <  1:835170/140‑1 (MQ=255)

                                                                                                  aCCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTGGCGGCGATGCTGGGGCAAATTGGCCt                           >  2:183068/1‑140 (MQ=255)

                                                                                                     aTTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTGGCGGCGATGCTGGGGCAAATTGGCCTGcc                        <  1:549327/140‑1 (MQ=255)

                                                                                                      ttCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTGGCGGCGATGCTGGGGCAAATTGGCCTGCCa                       >  1:133296/1‑140 (MQ=255)

                                                                                                          tGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTGGCGGCGATGCTGGGGCAAATTGGc                             >  2:246023/1‑130 (MQ=255)

                                                                                                          tGGGCTGGCGCGGCAGATGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTGGCGGCGATGCTGGGGCAAATTGGc                             <  1:246023/130‑1 (MQ=255)

                                                                                                                           tGGCGGCGAACAGAGCGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTGGCGGCGATGCTGGGGCAAATTGGCCTGCCAGGTGGTGGTTTTGGTTTtggc  <  1:838917/140‑1 (MQ=255)

TGGGTTTTGAGCAGTTCCTGCCGTATCTGCTGGGTGAGAAAGACGGTCAGCCGAAAGATGCCGCATGGGCTGAAAAACTGACCGGCATTGATGCCGAAACCATTCGTGGGCTGGCGCGGCAGATGGCGGCGAACAGAACGCAAATTATTGCTGGCTGGTGCGTGCAGCGTATGCAGCACGGTGAACAGTGGGCGTGGATGATTGTGGTTCTGGCGGCGATGCTGGGGCAAATTGGCCTGCCAGGTGGTGGTTTTGGTTTTGGC  >  NC_000913/1060217‑1060479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A