Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,612,237	C→T	C30Y (TGC→TAC)	yneG ←	conserved protein YneG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,612,237	0	C	T	100.0%	141.6 / NA	42	C30Y (TGC→TAC)	yneG	conserved protein YneG
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/22); total (20/22)

TGCTGAGCGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGCAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGAACGCCCTAACTGTTTCGTCAATTGTTC  >  NC_000913/1612105‑1612363

tgcagAGCGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTg                                                                                                                         >  1:387294/5‑140 (MQ=255)

   agAGCGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACg                                                                                                                      <  2:387294/139‑1 (MQ=255)

     aGCGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGct                                                                                                                    >  1:360129/1‑140 (MQ=255)

       cGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGctct                                                                                                                  >  2:422955/1‑140 (MQ=255)

       cGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGctct                                                                                                                  >  2:454757/1‑140 (MQ=255)

         aTAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCttt                                                                                                                <  2:717603/140‑1 (MQ=255)

          tAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTc                                                                                                               >  1:41637/1‑140 (MQ=255)

              cacCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGc                                                                                                           <  2:41637/140‑1 (MQ=255)

               acCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGcc                                                                                                          >  1:560558/1‑140 (MQ=255)

                ccGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGccc                                                                                                         <  2:560558/140‑1 (MQ=255)

                 cGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCa                                                                                                        >  1:494189/1‑140 (MQ=255)

                        ccGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGaaa                                                                                                 >  1:469711/1‑140 (MQ=255)

                           cgcATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCg                                                                                              >  2:865485/1‑140 (MQ=255)

                                gggggTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGccc                                                                                                         <  2:686013/124‑1 (MQ=255)

                                gggggTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGccc                                                                                                         >  1:686013/1‑124 (MQ=255)

                                 ggggTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCg                                                                                        <  1:790858/140‑1 (MQ=255)

                                       tGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAAttt                                                                                  >  1:726783/1‑140 (MQ=255)

                                         cTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTg                                                                                <  2:384872/140‑1 (MQ=255)

                                           tACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGaa                                                                              >  2:845671/1‑140 (MQ=255)

                                                  aTTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATa                                                                       >  1:439429/1‑140 (MQ=255)

                                                    tAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATaaa                                                                     <  1:208535/140‑1 (MQ=255)

                                                         ggTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAAcgcgc                                                                <  2:865089/140‑1 (MQ=255)

                                                          gTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAAcgcgcg                                                               <  2:360129/140‑1 (MQ=255)

                                                          gTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAAcgcgcg                                                               <  2:494189/140‑1 (MQ=255)

                                                          gTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAAcgcgcg                                                               <  2:439429/140‑1 (MQ=255)

                                                               gCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAagag                                                          <  1:422955/140‑1 (MQ=255)

                                                                 cGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATATTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTg                                                        <  2:517286/140‑1 (MQ=255)

                                                                  ggAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAag                                                            <  2:553677/135‑1 (MQ=255)

                                                                  ggAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAag                                                            >  1:553677/1‑135 (MQ=255)

                                                                     gCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATc                                                    >  1:879393/1‑140 (MQ=255)

                                                                     gCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATc                                                    >  1:160757/1‑140 (MQ=255)

                                                                        aGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCgag                                                 >  1:936720/1‑140 (MQ=255)

                                                                         gACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCgaga                                                <  1:845671/140‑1 (MQ=255)

                                                                              ttAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTg                                           <  2:726783/140‑1 (MQ=255)

                                                                                aGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCa                                         >  1:91050/1‑140 (MQ=255)

                                                                                  cGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCAtt                                       >  1:818602/1‑140 (MQ=255)

                                                                                             gccgcATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGaa                            <  2:879393/140‑1 (MQ=255)

                                                                                             gccgcATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGaa                            <  2:160757/140‑1 (MQ=255)

                                                                                               cgcATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGaa                            <  2:485221/138‑1 (MQ=255)

                                                                                               cgcATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGaa                            >  1:485221/1‑138 (MQ=255)

                                                                                                    gttgttCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGAACGCCCTa                     <  1:483515/140‑1 (MQ=255)

                                                                                                                       gcCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGAACGCCCTAACTGTTTCGTCAATTGTTc  <  2:936720/140‑1 (MQ=255)

TGCTGAGCGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGCAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGAACGCCCTAACTGTTTCGTCAATTGTTC  >  NC_000913/1612105‑1612363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A