Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F28 I1 R1
|
120 |
56.0 |
1898522 |
99.4% |
1887130 |
138.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,176,179 |
T→C |
N48S (AAC→AGC) |
gatZ ← |
tagatose‑1,6‑bisphosphate aldolase 2 subunit GatZ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,176,179 | 0 | T | C | 100.0%
| 79.8
/ NA
| 23 | N48S (AAC→AGC) | gatZ | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatZ |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/13); total (10/13) |
AGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGG > NC_000913/2176042‑2176305
|
agcagTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGctt > 2:711185/1‑140 (MQ=255)
agcagTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGctt > 1:793998/1‑140 (MQ=255)
agcagTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGctt > 1:138178/1‑140 (MQ=255)
tttGGTCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGt > 2:813430/1‑140 (MQ=255)
gagaATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGcgcg < 1:711185/140‑1 (MQ=255)
gaATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTg < 1:813430/140‑1 (MQ=255)
tCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATc > 2:371532/1‑140 (MQ=255)
tCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATc > 2:905739/1‑140 (MQ=255)
tCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATc > 1:563460/1‑140 (MQ=255)
aCCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAgc > 1:217273/1‑140 (MQ=255)
aTCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGa < 2:836661/140‑1 (MQ=255)
aTCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGa > 1:805335/1‑140 (MQ=255)
aTCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCaa < 2:563460/140‑1 (MQ=255)
aTCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCaa > 1:549912/1‑140 (MQ=255)
gTAAAAACAATTCCGCGAAAGTCTGCCGGTGTCATCCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACgg < 2:948739/140‑1 (MQ=255)
aaTTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGa < 1:26851/140‑1 (MQ=255)
gAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACt < 2:549912/140‑1 (MQ=255)
gTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAAc < 1:905739/140‑1 (MQ=255)
ccGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACa < 2:139413/127‑1 (MQ=255)
ccGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACa > 1:139413/1‑127 (MQ=255)
gtCATTCCGGTATAACCGCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGat < 2:28270/140‑1 (MQ=255)
gCCAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTAt > 2:424470/1‑140 (MQ=255)
cAAATTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGc < 1:371532/140‑1 (MQ=255)
aaaTTGATTGACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGcc > 1:570876/1‑140 (MQ=255)
attgattgACCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCgg < 2:793998/140‑1 (MQ=255)
ttgattgaCCTGGCTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCggg < 2:805335/140‑1 (MQ=255)
|
AGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGG > NC_000913/2176042‑2176305
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
