Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	106	90.3	3060316	99.2%	3035833	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	615,816	T→C	S554P (TCG→CCG)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	615,816	0	T	C	100.0%	191.9 / NA	51	S554P (TCG→CCG)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/30); total (21/30)

GGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATCACACGGC  >  NC_000913/615680‑615949

ggCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCcgccgc                                                                                                                                    <  1:891665/140‑1 (MQ=255)

ggCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCcgccgc                                                                                                                                    <  2:642936/140‑1 (MQ=255)

ggCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCcgccgc                                                                                                                                    >  1:1456352/1‑140 (MQ=255)

  cGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTg                                                                                                                                  >  2:3515/1‑140 (MQ=255)

        cACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAAtt                                                                                                                            <  2:465100/140‑1 (MQ=255)

          cTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTac                                                                                                                          <  1:110086/140‑1 (MQ=255)

             ccGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTaccac                                                                                                                       <  1:261793/140‑1 (MQ=255)

              cgcgCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTaccacc                                                                                                                      <  1:449849/140‑1 (MQ=255)

                cgcTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACcga                                                                                                                    >  1:1228039/1‑140 (MQ=255)

                     ggTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATc                                                                                                               <  1:1066075/140‑1 (MQ=255)

                            ttGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGcc                                                                                                        <  1:977344/140‑1 (MQ=255)

                             tGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCcg                                                                                                       <  1:973866/140‑1 (MQ=255)

                             tGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCcg                                                                                                       <  1:126357/140‑1 (MQ=255)

                               aCCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCcgcg                                                                                                     <  1:590884/140‑1 (MQ=255)

                                 ccTGGCACTCCATGCGATAGTTGACGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAAGGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCt                                                                                                   >  2:330814/1‑140 (MQ=255)

                                 ccTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGTCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCt                                                                                                   >  2:810005/1‑140 (MQ=255)

                                      cACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGc                                                                                              >  2:556129/1‑140 (MQ=255)

                                       aCTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCg                                                                                             <  1:407254/140‑1 (MQ=255)

                                                gATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCa                                                                                    <  1:757273/140‑1 (MQ=255)

                                                   aGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAAtt                                                                                 >  1:214292/1‑140 (MQ=255)

                                                     ttGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTa                                                                               >  1:746949/1‑140 (MQ=255)

                                                      tGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTaa                                                                              <  2:14999/140‑1 (MQ=255)

                                                           cTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGc                                                                         <  1:1302284/140‑1 (MQ=255)

                                                           cTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGc                                                                         <  2:1358934/140‑1 (MQ=255)

                                                            tGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGcc                                                                        >  1:896214/1‑140 (MQ=255)

                                                            tGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGcc                                                                        >  2:1321220/1‑140 (MQ=255)

                                                              gAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCtt                                                                      >  2:1083374/1‑140 (MQ=255)

                                                              gAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCtt                                                                      >  2:84564/1‑140 (MQ=255)

                                                              gAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCtt                                                                      <  2:782814/140‑1 (MQ=255)

                                                              gAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCtt                                                                      <  2:973889/140‑1 (MQ=255)

                                                              gAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCtt                                                                      >  2:698227/1‑140 (MQ=255)

                                                              gAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCtt                                                                      <  1:724717/140‑1 (MQ=255)

                                                                    ccTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCATCCGCCACTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCtat                                                                >  1:1178432/1‑140 (MQ=255)

                                                                          tACCGCTGGATACCGGCTCTCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCACCTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGcc                                                          <  1:810005/140‑1 (MQ=255)

                                                                            ccGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCATTGCTATAACGcccc                                                        <  2:600887/140‑1 (MQ=255)

                                                                              gCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGc                                                      >  2:70678/1‑140 (MQ=255)

                                                                                 ggATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTa                                                   <  1:330814/140‑1 (MQ=255)

                                                                                   aTACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTaca                                                 >  1:173350/1‑140 (MQ=255)

                                                                                       cGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGc                                             >  1:1177644/1‑140 (MQ=255)

                                                                                                   cGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTgcgc                                 <  2:746949/140‑1 (MQ=255)

                                                                                                       cGCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCt                             >  1:914731/1‑140 (MQ=255)

                                                                                                        gCCTGAAAATGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTg                            <  1:758203/140‑1 (MQ=255)

                                                                                                             aaaaTGATGCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACt                       <  1:84564/140‑1 (MQ=255)

                                                                                                                 tgatgCTGGAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTca                   >  1:1167956/1‑140 (MQ=255)

                                                                                                                         gAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCa           <  2:214292/140‑1 (MQ=255)

                                                                                                                         gAAGATGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCa           >  1:1247739/1‑140 (MQ=255)

                                                                                                                              tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      <  1:338450/140‑1 (MQ=255)

                                                                                                                              tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      <  1:3515/140‑1 (MQ=255)

                                                                                                                              tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      >  1:283755/1‑140 (MQ=255)

                                                                                                                              tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      <  2:182706/140‑1 (MQ=255)

                                                                                                                              tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      <  1:698227/140‑1 (MQ=255)

                                                                                                                              tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      >  2:1353512/1‑140 (MQ=255)

                                                                                                                              tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      >  1:784809/1‑140 (MQ=255)

                                                                                                                              tGCGCGTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATcaca      <  1:1109975/140‑1 (MQ=255)

                                                                                                                                 gcgTCCGCCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATCACACgg   <  2:1456352/140‑1 (MQ=255)

                                                                                                                                 atggccgccgCGGTTAAATACCACCGACTGAGAACTGCCGCGCTTAAGCGCTGTGCCCAATTTAACAAGCCTTTGCTCTAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATCACACgg   <  2:722983/136‑1 (MQ=255)

                                                                                                                                  cgtccgccGCTGTTAATTACCACCGACGATCCACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATCACACGGc  <  2:914731/140‑1 (MQ=255)

GGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAATTACCACCGACGATCAACTGCCGCGCTTTAGCGATGTTCCCAATTTAACAAGCCTTTGCTATAACGCCCCGCTTACACCGCAGGGCAGTGCGCCGCTGCAACTTTCACAACCGCATCACACGGC  >  NC_000913/615680‑615949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A