Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 106 90.3 3060316 99.2% 3035833 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,309,798 A→G V203A (GTG→GCG)  ada ← DNA‑binding transcriptional dual regulator/DNA repair protein Ada

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,309,7980AG100.0% 196.6 / NA 54V203A (GTG→GCG) adaDNA‑binding transcriptional dual regulator/DNA repair protein Ada
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/24);  total (30/24)

CATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGG  >  NC_000913/2309670‑2309928
                                                                                                                                |                                                                                                                                  
caTGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGa                                                                                                                         >  1:696587/1‑140 (MQ=255)
 aTGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGAc                                                                                                                        >  1:236617/1‑140 (MQ=255)
  tGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGAcc                                                                                                                       <  2:1233445/140‑1 (MQ=255)
  tGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGAcc                                                                                                                       >  1:258953/1‑140 (MQ=255)
  tGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGAcc                                                                                                                       <  2:196785/140‑1 (MQ=255)
      gCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGc                                                                                                                   >  1:354688/1‑140 (MQ=255)
       cTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCt                                                                                                                  <  2:154391/140‑1 (MQ=255)
           aaCATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTcaca                                                                                                              <  2:1267070/140‑1 (MQ=255)
              aTCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATc                                                                                                           >  1:855973/1‑140 (MQ=255)
               tCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATca                                                                                                          >  1:1341259/1‑140 (MQ=255)
               tCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATca                                                                                                          >  1:742852/1‑140 (MQ=255)
                 aGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATcagc                                                                                                        >  1:388936/1‑140 (MQ=255)
                 aGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATcagc                                                                                                        <  2:170657/140‑1 (MQ=255)
                 aGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCCGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATcagc                                                                                                        >  2:460307/1‑140 (MQ=255)
                  gATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATcagcc                                                                                                       <  2:768534/140‑1 (MQ=255)
                   aTCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATcagcca                                                                                                      <  2:121485/140‑1 (MQ=255)
                                ttGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCAc                                                                                         <  2:351010/140‑1 (MQ=255)
                                 tGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCAcc                                                                                        >  1:927280/1‑140 (MQ=255)
                                  gTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCg                                                                                       >  1:368651/1‑140 (MQ=255)
                                    cGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGcc                                                                                     <  2:1341259/140‑1 (MQ=255)
                                                      gctgcAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGa                                                                   >  1:926490/1‑140 (MQ=255)
                                                       ctgcAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGAc                                                                  >  1:302741/1‑140 (MQ=255)
                                                           aaCTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGaa                                                              <  2:390148/140‑1 (MQ=255)
                                                               cGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGt                                                          >  1:820621/1‑140 (MQ=255)
                                                                gCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGtt                                                         >  2:24994/1‑140 (MQ=255)
                                                                   gATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAg                                                      <  2:155404/140‑1 (MQ=255)
                                                                         tgtCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCa                                                >  2:96606/1‑140 (MQ=255)
                                                                             gcgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGcc                                            <  2:170669/140‑1 (MQ=255)
                                                                              cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           >  1:862027/1‑140 (MQ=255)
                                                                              cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           >  2:1525320/1‑140 (MQ=255)
                                                                              cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           >  2:844747/1‑140 (MQ=255)
                                                                              cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           >  2:676998/1‑140 (MQ=255)
                                                                              cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           <  1:286693/140‑1 (MQ=255)
                                                                              cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           >  2:1194825/1‑140 (MQ=255)
                                                                              cgTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGccc                                           >  1:189858/1‑140 (MQ=255)
                                                                                 catcatCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGc                                        <  2:696587/140‑1 (MQ=255)
                                                                                  atcatcGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCg                                       >  2:1525941/1‑140 (MQ=255)
                                                                                         cccAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTc                                >  1:969568/1‑140 (MQ=255)
                                                                                           cAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAg                              <  2:855973/140‑1 (MQ=255)
                                                                                             gCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCt                            <  2:368651/140‑1 (MQ=255)
                                                                                                atatCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTg                         <  2:258953/140‑1 (MQ=255)
                                                                                                 tatCGCGCAAATCCCCCGCTCGCTTTCTGCCGCCAGACAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGc                        <  1:688917/140‑1 (MQ=255)
                                                                                                      cgcAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAg                   >  2:701134/1‑140 (MQ=255)
                                                                                                      cgcAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAg                   >  1:12450/1‑140 (MQ=255)
                                                                                                       gcAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGt                  >  1:1324073/1‑140 (MQ=255)
                                                                                                        cAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTa                 >  1:243245/1‑140 (MQ=255)
                                                                                                        cAAATCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTa                 >  2:854121/1‑140 (MQ=255)
                                                                                                           aTCCCCCGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAAct              >  2:959161/1‑140 (MQ=255)
                                                                                                               cccGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAActgctg          <  1:1525941/140‑1 (MQ=255)
                                                                                                               cccGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAActgctg          <  1:737864/140‑1 (MQ=255)
                                                                                                               cccGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAActgctg          <  1:96606/140‑1 (MQ=255)
                                                                                                               cccGCTCGCTTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAActgctg          <  2:926490/140‑1 (MQ=255)
                                                                                                                  gctcgctTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAActgctgctg       <  1:145630/140‑1 (MQ=255)
                                                                                                                      gctTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCgg   <  2:388936/140‑1 (MQ=255)
                                                                                                                       ctTTCTGCCGCCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCggg  >  1:64219/1‑140 (MQ=255)
                                                                                                                                |                                                                                                                                  
CATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGG  >  NC_000913/2309670‑2309928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A