Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 106 90.3 3060316 99.2% 3035833 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,602,0101.C98.7% 275.8 / ‑3.2 76coding (193/2019 nt)hyfBhydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (36/39);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-01

TCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCA  >  NC_000913/2601871‑2602142
                                                                                                                                            |                                                                                                                                     
tCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTg                                                                                                                                        >  1:1217415/1‑140 (MQ=255)
tCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTg                                                                                                                                        >  1:593607/1‑140 (MQ=255)
tCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTg                                                                                                                                        >  2:542022/1‑140 (MQ=255)
  tGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑cc                                                                                                                                    >  2:1077026/1‑140 (MQ=255)
    gCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑cccc                                                                                                                                  >  2:1280/1‑140 (MQ=255)
    gCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑cccc                                                                                                                                  >  1:847154/1‑140 (MQ=255)
    gCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑cccc                                                                                                                                  <  1:205144/140‑1 (MQ=255)
     cTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑ccccc                                                                                                                                 >  1:1504997/1‑140 (MQ=255)
     cTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑ccccc                                                                                                                                 <  1:1300536/140‑1 (MQ=255)
               tttACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGaa                                                                                                                        >  1:514474/1‑140 (MQ=255)
                   cTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCCTTTTGAAttt                                                                                                                     >  2:32476/1‑140 (MQ=255)
                    tCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGc                                                                                                                   <  2:1443915/140‑1 (MQ=255)
                    tCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGc                                                                                                                   <  1:1493302/140‑1 (MQ=255)
                       gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGa                                                                                                                >  1:344874/1‑140 (MQ=255)
                         atGGACAGCCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGTGGCGTGATTGGCATCATCCGCGGAATTACGCAGTTACATGCGGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGAtt                                                                                                              <  2:1132505/139‑1 (MQ=255)
                             aCAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAAc                                                                                                          <  2:1217415/140‑1 (MQ=255)
                               aGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAAccc                                                                                                        <  2:336578/140‑1 (MQ=255)
                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTg                                                                                                      >  1:874830/1‑140 (MQ=255)
                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTg                                                                                                      >  1:847645/1‑140 (MQ=255)
                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTg                                                                                                      >  2:176310/1‑140 (MQ=255)
                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTg                                                                                                      >  1:545119/1‑140 (MQ=255)
                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTg                                                                                                      >  2:788417/1‑140 (MQ=255)
                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTg                                                                                                      >  1:753987/1‑140 (MQ=255)
                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCT‑GTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGc                                                                                                     >  1:613065/1‑140 (MQ=255)
                                  cTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGc                                                                                                     >  2:650355/1‑140 (MQ=255)
                                  cTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGc                                                                                                     >  1:1325248/1‑140 (MQ=255)
                                  cTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGc                                                                                                     >  2:728319/1‑140 (MQ=255)
                                   tGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                    <  2:593607/140‑1 (MQ=255)
                                      cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAAt                                                                                                 >  1:1505614/1‑140 (MQ=255)
                                         ttAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGa                                                                                              >  1:1428720/1‑140 (MQ=255)
                                         ttAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGa                                                                                              <  2:671640/140‑1 (MQ=255)
                                          tAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGAt                                                                                             <  1:940364/140‑1 (MQ=255)
                                               tttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCct                                                                                        >  2:824627/1‑140 (MQ=255)
                                               tttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGGAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGa                                                                                              >  2:954935/1‑134 (MQ=255)
                                               tttCCGGCATCACAACGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGa                                                                                              <  1:954935/134‑1 (MQ=255)
                                                 tCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                                                      >  2:821060/1‑140 (MQ=255)
                                                             tCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATgg                                                                          <  1:931834/140‑1 (MQ=255)
                                                               gCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTg                                                                        <  2:461036/140‑1 (MQ=255)
                                                                cTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGc                                                                       <  2:802823/140‑1 (MQ=255)
                                                                  ggtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTg                                                                     <  1:871952/140‑1 (MQ=255)
                                                                   gtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTgg                                                                    >  2:1392997/1‑140 (MQ=255)
                                                                   gtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAAATTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTgg                                                                    >  2:595519/1‑140 (MQ=255)
                                                                    tggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGt                                                                   <  1:1435341/140‑1 (MQ=255)
                                                                     gggCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGtt                                                                  <  1:1504361/140‑1 (MQ=255)
                                                                        cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtc                                                               >  1:168369/1‑140 (MQ=255)
                                                                          gcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtctc                                                             >  1:19432/1‑140 (MQ=255)
                                                                           cgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTcc                                                            >  1:1346175/1‑140 (MQ=255)
                                                                            gTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCt                                                           <  2:548851/140‑1 (MQ=255)
                                                                                    catcatCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                                                   <  1:821060/140‑1 (MQ=255)
                                                                                       catcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtggtg                                                >  1:244964/1‑140 (MQ=255)
                                                                                        atcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtggtgg                                               <  2:1504997/140‑1 (MQ=255)
                                                                                         tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                                              >  2:619978/1‑140 (MQ=255)
                                                                                         tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                                              <  2:9152/140‑1 (MQ=255)
                                                                                         tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                                              <  2:344874/140‑1 (MQ=255)
                                                                                            gCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTg                                           <  1:661210/140‑1 (MQ=255)
                                                                                             cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                          <  1:1077026/140‑1 (MQ=255)
                                                                                             cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                          >  1:1496360/1‑140 (MQ=255)
                                                                                              ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                          <  1:32476/140‑1 (MQ=255)
                                                                                              ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGtt                                         <  1:1280/140‑1 (MQ=255)
                                                                                               gAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                        <  2:823104/140‑1 (MQ=255)
                                                                                               gAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                        <  1:352783/140‑1 (MQ=255)
                                                                                                aaTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtct                                       <  1:176310/140‑1 (MQ=255)
                                                                                                aaTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtct                                       <  2:753987/140‑1 (MQ=255)
                                                                                                 aTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctc                                      <  1:1003785/140‑1 (MQ=255)
                                                                                                 aTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctc                                      <  1:488306/140‑1 (MQ=255)
                                                                                                  ttACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctct                                     <  1:1469087/140‑1 (MQ=255)
                                                                                                  ttACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctct                                     >  1:988954/1‑140 (MQ=255)
                                                                                                  ttACGCAATTACATGCT‑GTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                                    <  2:613065/140‑1 (MQ=255)
                                                                                                   tACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                                    <  1:174509/140‑1 (MQ=255)
                                                                                                      gCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTAt                                 <  2:500862/140‑1 (MQ=255)
                                                                                                        aaTTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTc                               <  1:728319/140‑1 (MQ=255)
                                                                                                          ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                             <  2:1502600/140‑1 (MQ=255)
                                                                                                          ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                             <  2:1428720/140‑1 (MQ=255)
                                                                                                          ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                             >  1:1001307/1‑140 (MQ=255)
                                                                                                          ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                             >  2:1369930/1‑140 (MQ=255)
                                                                                                             cATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGa                          >  1:238025/1‑140 (MQ=255)
                                                                                                                  tGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtat                     >  2:222735/1‑140 (MQ=255)
                                                                                                                   ggTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtata                    <  2:1505614/140‑1 (MQ=255)
                                                                                                                    gtgtAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTatat                   >  2:388372/1‑140 (MQ=255)
                                                                                                                      gtAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATgc                 <  1:1287202/140‑1 (MQ=255)
                                                                                                                            ttAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAAt           >  1:1445476/1‑140 (MQ=255)
                                                                                                                              aGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc         >  2:107437/1‑140 (MQ=255)
                                                                                                                              aGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc         >  2:1056854/1‑140 (MQ=255)
                                                                                                                              aGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc         >  2:1387446/1‑140 (MQ=255)
                                                                                                                               gTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACg        <  2:874830/140‑1 (MQ=255)
                                                                                                                                    ccGTTTTG‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCa  >  2:297642/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                     
TCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCA  >  NC_000913/2601871‑2602142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A