Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 106 90.3 3060316 99.2% 3035833 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,605,262 C→T A151V (GCG→GTG)  hyfD → hydrogenase 4 component D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,605,2620CT95.5% 207.4 / NA 67A151V (GCG→GTG) hyfDhydrogenase 4 component D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (24/40);  minor base G (3/0);  total (27/40)
Fisher's exact test for biased strand distribution p-value = 6.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTGCCGCCGCCACGCTGTTTTTGCAGACCGGAACGTTTGC  >  NC_000913/2605129‑2605391
                                                                                                                                     |                                                                                                                                 
cacCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGAt                                                                                                                             <  2:898605/140‑1 (MQ=255)
cacCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGAt                                                                                                                             <  2:33989/140‑1 (MQ=255)
cacCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGAt                                                                                                                             <  2:352079/140‑1 (MQ=255)
cacCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGAt                                                                                                                             <  2:1441283/140‑1 (MQ=255)
cacCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGAt                                                                                                                             <  2:1080842/140‑1 (MQ=255)
cacCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGAt                                                                                                                             <  2:1020451/140‑1 (MQ=255)
cacCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGAt                                                                                                                             <  1:685725/140‑1 (MQ=255)
cacCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGAt                                                                                                                             <  1:482169/140‑1 (MQ=255)
cacCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGAt                                                                                                                             <  2:510918/140‑1 (MQ=255)
cacCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGAt                                                                                                                             <  1:1226826/140‑1 (MQ=255)
            ggCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCa                                                                                                                 <  2:195719/140‑1 (MQ=255)
             gCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCag                                                                                                                >  2:490476/1‑140 (MQ=255)
             gCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGATTTTTGAAATTACAGGCGGCTGCTCCTGGGGGTTGATCAGTTATTACCag                                                                                                                >  1:187113/1‑140 (MQ=255)
               aCGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCagag                                                                                                              <  2:79159/140‑1 (MQ=255)
               aCGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCagag                                                                                                              <  1:930556/140‑1 (MQ=255)
                cGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGc                                                                                                             >  1:1175903/1‑140 (MQ=255)
                cGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGc                                                                                                             >  1:532267/1‑140 (MQ=255)
                   aTCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGGGTTGATCAGTTATTACCAGAGCGAt                                                                                                          >  2:816667/1‑140 (MQ=255)
                   aTCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGAATGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGGTATTACCAGAGCGAt                                                                                                          >  1:1513293/1‑140 (MQ=255)
                      gTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATaaa                                                                                                       <  2:160076/140‑1 (MQ=255)
                      gTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATaaa                                                                                                       <  2:176809/140‑1 (MQ=255)
                               cATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGt                                                                                              <  2:1382449/140‑1 (MQ=255)
                               cATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGt                                                                                              >  2:442505/1‑140 (MQ=255)
                                aTTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGtt                                                                                             >  1:1433900/1‑140 (MQ=255)
                                aTTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCCCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGtt                                                                                             >  1:144080/1‑140 (MQ=255)
                                 tttttACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTc                                                                                            <  2:245666/140‑1 (MQ=255)
                                 tttttACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTc                                                                                            <  2:1175903/140‑1 (MQ=255)
                                          gtgtTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTaaa                                                                                   >  1:378833/1‑140 (MQ=255)
                                            gtTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGCCGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGGTGATCAGTTATTACCAGAGCGATAAAGCGCCGCGGTCAGCACTAAAAg                                                                                 >  2:1480333/1‑140 (MQ=255)
                                            gtTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAg                                                                                 <  2:532267/140‑1 (MQ=255)
                                             tttATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGc                                                                                >  2:32033/1‑140 (MQ=255)
                                              ttATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAACGATAAAGCGCAGCGTTCAGCACTAAAAGCg                                                                               <  2:1514548/140‑1 (MQ=255)
                                               tATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGGGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGt                                                                              >  2:1481228/1‑140 (MQ=255)
                                                aTCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGtt                                                                             <  1:1294228/140‑1 (MQ=255)
                                                aTCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGtt                                                                             <  2:271473/140‑1 (MQ=255)
                                                   ggCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACt                                                                          <  2:467380/140‑1 (MQ=255)
                                                    gcgcGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACtt                                                                         <  1:1462724/140‑1 (MQ=255)
                                                    gcgcGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACtt                                                                         <  1:1188184/140‑1 (MQ=255)
                                                     cgcgATGGCGGGACTGGTACTCTCCTTGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTa                                                                        >  2:1270037/1‑140 (MQ=255)
                                                     cgcgATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTa                                                                        <  1:1254083/140‑1 (MQ=255)
                                                     cgcgATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTa                                                                        <  2:137637/140‑1 (MQ=255)
                                                     cgcgATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTa                                                                        >  1:674737/1‑140 (MQ=255)
                                                     cgcgATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTa                                                                        >  1:503103/1‑140 (MQ=255)
                                                     cgcgATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTa                                                                        <  1:1299272/140‑1 (MQ=255)
                                                      gcgATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTAt                                                                       <  1:950058/140‑1 (MQ=255)
                                                       cgATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATc                                                                      <  1:1270037/140‑1 (MQ=255)
                                                                       aCTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGt                                                      <  2:1087675/140‑1 (MQ=255)
                                                                        ctctCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTTATCAGTTATTACCAGAACGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGtt                                                     <  2:681718/140‑1 (MQ=255)
                                                                          ctcctcGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTgg                                                   >  2:1221166/1‑140 (MQ=255)
                                                                            cctcGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTgggg                                                 <  2:1093903/140‑1 (MQ=255)
                                                                            cctcGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTAGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTgggg                                                 <  2:1104422/140‑1 (MQ=255)
                                                                             ctcGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTggggg                                                >  1:886638/1‑139 (MQ=255)
                                                                               cGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTg                                              <  1:252305/140‑1 (MQ=255)
                                                                                     tgctCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCtt                                        >  2:923758/1‑140 (MQ=255)
                                                                                     tgctCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGGTGTATCtt                                        >  2:643435/1‑140 (MQ=255)
                                                                                     tgctCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTAGGGGATGTATCtt                                        >  2:1488396/1‑140 (MQ=255)
                                                                                               gttgttgttTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGGTTGGGGTGTATCTTgccgccgccc                              >  2:888139/1‑139 (MQ=255)
                                                                                                 tgttgttTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTGCCGCCGCCACg                            >  1:1412152/1‑140 (MQ=255)
                                                                                                  gttgttTTTTGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTGCCGCCGCCACGc                           <  1:389662/140‑1 (MQ=255)
                                                                                                      ttttttGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGGTGGGGTTGCATTTTTCCGCCGCCCCGccggt                       >  2:422016/1‑136 (MQ=255)
                                                                                                        ttttGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGGTGTATCTTGCCGCCGCCACGCTGtttt                     >  1:383985/1‑140 (MQ=255)
                                                                                                          ttGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTGCCGCCGCCACGCTGTTTTTg                   >  1:97994/1‑140 (MQ=255)
                                                                                                          ttGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGGTGTATCTTGCCGCCGCCACGCTGtttttt                   >  1:522142/1‑139 (MQ=255)
                                                                                                          ttGAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGGTGTATCTTGCCGCCGCCACCCTGTTTTTg                   >  2:102540/1‑140 (MQ=255)
                                                                                                            gAAATTACAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTGCCGCCGCCACGCTGTTTTTGCa                 <  1:32033/140‑1 (MQ=255)
                                                                                                                  aCAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTGCCGCCGCCACGCTGTTTTTGCAGACCgg           <  1:107311/140‑1 (MQ=255)
                                                                                                                   cAGGCGGCTGCTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTGCCGCCGCCACGCTGTTTTTGCAGACCGGa          <  1:103228/140‑1 (MQ=255)
                                                                                                                           tgctCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTGCCGCCGCCACGCTGTTTTTGCAGACCGGAACGTTTgc  <  1:1480333/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                 
CACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTGCCGCCGCCACGCTGTTTTTGCAGACCGGAACGTTTGC  >  NC_000913/2605129‑2605391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A