Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	581,442	C→T	pseudogene (89/309 nt)	aaaD →	DLP12 prophage; protein AaaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	581,442	0	C	T	98.6%	241.9 / ‑4.2	71	pseudogene (89/309 nt)	aaaD	DLP12 prophage; protein AaaD
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (36/34); total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

CATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGT  >  NC_000913/581307‑581574

cATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGtt                                                                                                                                  >  1:96059/1‑140 (MQ=255)

cATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGtt                                                                                                                                  >  2:116314/1‑140 (MQ=255)

cATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGtt                                                                                                                                  >  2:224440/1‑140 (MQ=255)

cATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGtt                                                                                                                                  >  2:748223/1‑140 (MQ=255)

 aTGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTg                                                                                                                                 >  2:327956/1‑140 (MQ=255)

 aTGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTg                                                                                                                                 >  2:498762/1‑140 (MQ=255)

  tGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGa                                                                                                                                >  2:887698/1‑140 (MQ=255)

  tGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGa                                                                                                                                <  2:895831/140‑1 (MQ=255)

   gAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGaa                                                                                                                               <  1:887698/140‑1 (MQ=255)

    aaCAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAAt                                                                                                                              <  1:830734/140‑1 (MQ=255)

           cagcCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGa                                                                                                                       <  2:595661/140‑1 (MQ=255)

           cagcCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGa                                                                                                                       <  2:514434/140‑1 (MQ=255)

             gcCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATg                                                                                                                     <  1:563276/140‑1 (MQ=255)

              ccGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGc                                                                                                                    >  2:368783/1‑140 (MQ=255)

                 cgcTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTaa                                                                                                                 >  2:894773/1‑140 (MQ=255)

                   cTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCgg                                                                                                                        >  1:791947/1‑131 (MQ=255)

                   cTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCgg                                                                                                                        <  2:791947/131‑1 (MQ=255)

                      gATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACt                                                                                                            <  1:667194/140‑1 (MQ=255)

                       aTGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTa                                                                                                           <  1:327956/140‑1 (MQ=255)

                              gATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCg                                                                                                    >  2:571422/1‑140 (MQ=255)

                                tACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGaa                                                                                                  >  1:536341/1‑140 (MQ=255)

                                tACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGaa                                                                                                  >  2:1037143/1‑140 (MQ=255)

                                tACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGaa                                                                                                  >  1:427680/1‑140 (MQ=255)

                                  ccGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAg                                                                                                <  1:224440/140‑1 (MQ=255)

                                   cgatatagCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGa                                                                                               >  2:504213/8‑140 (MQ=255)

                                    ggggTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGaa                                                                                              >  1:716154/1‑140 (MQ=255)

                                     gggTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTa                                                                                                           >  2:8905/1‑126 (MQ=255)

                                     gggTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTa                                                                                                           <  1:8905/126‑1 (MQ=255)

                                     gggTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAAt                                                                                             >  1:474702/1‑140 (MQ=255)

                                     gggTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAAt                                                                                             >  2:674039/1‑140 (MQ=255)

                                        ttGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCg                                                                                          <  1:116314/140‑1 (MQ=255)

                                        ttGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCg                                                                                          <  1:748223/140‑1 (MQ=255)

                                        tagCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCg                                                                                          <  1:504213/138‑1 (MQ=255)

                                         tGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGc                                                                                         <  2:734631/140‑1 (MQ=255)

                                          gCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCa                                                                                        >  1:768758/1‑140 (MQ=255)

                                            tGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATa                                                                                      >  1:446404/1‑140 (MQ=255)

                                                  aaTATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAACCCGCATACCAGGa                                                                                >  1:702776/1‑140 (MQ=255)

                                                   atatatCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGaa                                                                               <  2:536341/140‑1 (MQ=255)

                                                   atatatCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGaa                                                                               <  2:446404/140‑1 (MQ=255)

                                                     atatCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAgg                                                                             >  2:781849/1‑140 (MQ=255)

                                                         cGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGgcgc                                                                         >  1:222337/1‑140 (MQ=255)

                                                            aCAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTgg                                                                      >  1:272974/1‑140 (MQ=255)

                                                             gaGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCAGGGCTTCGCTCACTGTTCAGGCCGGGGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTggg                                                                     <  2:746642/139‑1 (MQ=255)

                                                                 cAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAac                                                                 >  2:48512/1‑140 (MQ=255)

                                                                        aCAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGccc                                                          >  2:190199/1‑140 (MQ=255)

                                                                         cAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCt                                                         <  2:950188/140‑1 (MQ=255)

                                                                          aGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACt                                                              >  2:210637/1‑134 (MQ=255)

                                                                          aGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACt                                                              <  1:210637/134‑1 (MQ=255)

                                                                          aGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCtt                                                        >  2:554240/1‑140 (MQ=255)

                                                                              tGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAg                                                    >  1:669440/1‑140 (MQ=255)

                                                                              tGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAg                                                    >  2:725365/1‑140 (MQ=255)

                                                                               gCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGc                                                   <  1:729762/140‑1 (MQ=255)

                                                                               gCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGc                                                   <  1:251526/140‑1 (MQ=255)

                                                                                 ggCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCgg                                                 <  2:474702/140‑1 (MQ=255)

                                                                                  gCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCggg                                                <  1:828794/140‑1 (MQ=255)

                                                                                      tttGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCcat                                            <  1:190199/140‑1 (MQ=255)

                                                                                             gcgcCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAAt                                     <  1:498762/140‑1 (MQ=255)

                                                                                                 cGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGa                                 <  2:272974/140‑1 (MQ=255)

                                                                                                  gggCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGAt                                <  1:822462/140‑1 (MQ=255)

                                                                                                   ggCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATg                               >  2:556183/1‑140 (MQ=255)

                                                                                                   ggCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAACAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATg                               >  2:359760/1‑140 (MQ=255)

                                                                                                       tCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCa                           <  2:128/140‑1 (MQ=255)

                                                                                                       tCGCTCACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCa                           <  1:554240/140‑1 (MQ=255)

                                                                                                            cACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGAc                      <  1:556183/140‑1 (MQ=255)

                                                                                                            cACTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGAc                      <  2:96059/140‑1 (MQ=255)

                                                                                                              cTGTTCAGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTa                    >  2:304873/1‑140 (MQ=255)

                                                                                                                    aGGCCGGAGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCg              <  2:669440/140‑1 (MQ=255)

                                                                                                                           aGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTg       >  2:476938/1‑140 (MQ=255)

                                                                                                                           aGCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTg       >  1:455152/1‑140 (MQ=255)

                                                                                                                            gCCACAGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGa      <  1:571422/140‑1 (MQ=255)

                                                                                                                                caGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAAtgt  >  2:853937/1‑140 (MQ=255)

                                                                                                                                caGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAAt    <  2:326484/138‑1 (MQ=255)

                                                                                                                                caGACCGTCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAAt    >  1:326484/1‑138 (MQ=255)

CATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGT  >  NC_000913/581307‑581574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A