Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	603,108	A→G	M384V (ATG→GTG)	pheP →	phenylalanine:H(+) symporter PheP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	603,108	0	A	G	100.0%	166.8 / NA	46	M384V (ATG→GTG)	pheP	phenylalanine:H(+) symporter PheP
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/24); total (22/24)

GGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTG  >  NC_000913/602973‑603244

ggTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGAt                                                                                                                                      <  2:615783/140‑1 (MQ=255)

        gATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTgg                                                                                                                              <  2:160983/140‑1 (MQ=255)

              cTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGAGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATc                                                                                                                        >  1:75026/1‑140 (MQ=255)

                cGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTg                                                                                                                      <  2:265046/140‑1 (MQ=255)

                  cTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCg                                                                                                                    <  2:396655/140‑1 (MQ=255)

                          ttCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTg                                                                                                            <  2:449620/140‑1 (MQ=255)

                           tCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGc                                                                                                           >  1:416016/1‑140 (MQ=255)

                                 gCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGAt                                                                                                     >  2:538707/1‑140 (MQ=255)

                                    aTCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCg                                                                                                  >  1:335196/1‑140 (MQ=255)

                                     tCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGa                                                                                                 >  1:362494/1‑140 (MQ=255)

                                           cGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAg                                                                                           <  2:765445/140‑1 (MQ=255)

                                                   gtgtTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGAt                                                                                                     >  1:297172/1‑122 (MQ=255)

                                                   gtgtTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGAt                                                                                                     <  2:297172/122‑1 (MQ=255)

                                                   gtgtTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGa                                                                                   >  1:477069/1‑140 (MQ=255)

                                                     gtTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGaaa                                                                                 >  2:318634/1‑140 (MQ=255)

                                                      ttAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAac                                                                                <  2:579631/140‑1 (MQ=255)

                                                           cAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGt                                                                           <  2:114684/140‑1 (MQ=255)

                                                              cTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTa                                                                        >  1:640894/1‑140 (MQ=255)

                                                                aTCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAg                                                                      >  2:452977/1‑140 (MQ=255)

                                                                 tCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAgg                                                                     <  1:857329/140‑1 (MQ=255)

                                                                   tgctgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgcg                                                                   <  2:131844/140‑1 (MQ=255)

                                                                   tgctgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgcg                                                                   >  2:133106/1‑140 (MQ=255)

                                                                   tgctgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgcg                                                                   <  2:477069/140‑1 (MQ=255)

                                                                   tgctgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgcg                                                                   <  2:818786/140‑1 (MQ=255)

                                                                    gctgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgc                                                                    <  2:1000784/138‑1 (MQ=255)

                                                                    gctgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgc                                                                    >  1:1000784/1‑138 (MQ=255)

                                                                      tgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgcg                                                                   <  1:878897/137‑1 (MQ=255)

                                                                      tgcCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGgcg                                                                   >  2:878897/1‑137 (MQ=255)

                                                                             aaaaGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATc                                                         <  1:452977/140‑1 (MQ=255)

                                                                                   ctttGGTGTGCTGATGGCGCGGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGGCGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCg                                                   <  2:640894/139‑1 (MQ=255)

                                                                                    tttGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCgg                                                  <  1:195180/140‑1 (MQ=255)

                                                                                         tCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACt                                             >  1:1028469/1‑140 (MQ=255)

                                                                                            gctgATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTAtc                                          >  2:1038900/1‑140 (MQ=255)

                                                                                             ctgATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTAtct                                         <  1:32051/140‑1 (MQ=255)

                                                                                                  ggCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCg                                                   >  1:528548/1‑125 (MQ=255)

                                                                                                  ggCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTg                                      <  1:1010776/138‑1 (MQ=255)

                                                                                                  ggCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTg                                      >  2:1010776/1‑138 (MQ=255)

                                                                                                  ggCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCa                                    >  1:59370/1‑140 (MQ=255)

                                                                                                  ggCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTCCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCg                                                   <  2:528548/125‑1 (MQ=255)

                                                                                                     gcTGGTGGTAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTg                                 <  1:287114/140‑1 (MQ=255)

                                                                                                          tggtAGCAACGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTc                            >  1:386068/1‑140 (MQ=255)

                                                                                                                   cGCTGCTGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATg                   >  2:956378/1‑140 (MQ=255)

                                                                                                                    gctgctGTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGa                  >  2:105805/1‑140 (MQ=255)

                                                                                                                     ctgctgTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGAt                 <  1:318634/140‑1 (MQ=255)

                                                                                                                       gctgTTGAACTGGATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGAttt               <  1:824119/140‑1 (MQ=255)

                                                                                                                                   gATTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGAtgt   >  2:97994/1‑140 (MQ=255)

                                                                                                                                    aTTGTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGAtgtg  <  2:940432/140‑1 (MQ=255)

GGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTG  >  NC_000913/602973‑603244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A