Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,075,403	A→G	C1160C (TGT→TGC)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,075,403	0	A	G	100.0%	194.8 / NA	52	C1160C (TGT→TGC)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/24); total (28/24)

CGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGC  >  NC_000913/1075274‑1075532

cgcATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCt                                                                                                                         >  2:1050392/1‑140 (MQ=255)

     ccTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAAt                                                                                                                    >  2:558402/1‑140 (MQ=255)

      cTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAAtt                                                                                                                   >  1:74616/1‑140 (MQ=255)

         cTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTg                                                                                                                >  1:857397/1‑140 (MQ=255)

            atcatcGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGAc                                                                                                             <  2:754563/140‑1 (MQ=255)

               atcGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGAt                                                                                                          >  1:550139/1‑140 (MQ=255)

                 cGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGAttt                                                                                                        >  1:846056/1‑140 (MQ=255)

                            aacaCGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTc                                                                                             >  2:992188/1‑140 (MQ=255)

                            aacaCGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTc                                                                                             <  1:992188/140‑1 (MQ=255)

                              cacGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTccc                                                                                           <  1:1050392/140‑1 (MQ=255)

                              cacGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTccc                                                                                           >  2:429924/1‑140 (MQ=255)

                              cacGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTccc                                                                                           <  1:801978/140‑1 (MQ=255)

                              cacGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTccc                                                                                           >  1:534340/1‑140 (MQ=255)

                                 gcgcTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCa                                                                                        >  2:136452/1‑140 (MQ=255)

                                 gcgcTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCa                                                                                        <  1:846669/140‑1 (MQ=255)

                                 gcgcTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCa                                                                                        >  1:362783/1‑140 (MQ=255)

                                   gcTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGt                                                                                      >  2:36389/1‑140 (MQ=255)

                                   gcTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGCTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGt                                                                                      >  1:368061/1‑140 (MQ=255)

                                              agcagcGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCgg                                                                           <  2:534340/140‑1 (MQ=255)

                                                cagcGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCt                                                                         <  2:118146/140‑1 (MQ=255)

                                                         ggTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATg                                                                <  2:857397/140‑1 (MQ=255)

                                                               gCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCtt                                                          >  1:919454/1‑140 (MQ=255)

                                                                cGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCgg                                                                           >  1:807842/1‑122 (MQ=255)

                                                                cGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCgg                                                                           <  2:807842/122‑1 (MQ=255)

                                                                    cACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAAc                                                     >  2:898694/1‑140 (MQ=255)

                                                                    cACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAAc                                                     >  2:743521/1‑140 (MQ=255)

                                                                     aCCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACt                                                    >  1:90262/1‑140 (MQ=255)

                                                                         gTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTgcgc                                                <  2:919454/140‑1 (MQ=255)

                                                                          tCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCa                                               >  2:551270/1‑140 (MQ=255)

                                                                           cGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCAt                                              <  1:576264/140‑1 (MQ=255)

                                                                           cGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCAt                                              <  1:898694/140‑1 (MQ=255)

                                                                                 cGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCg                                        >  2:482214/1‑140 (MQ=255)

                                                                                  ggCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCgg                                       <  2:90262/140‑1 (MQ=255)

                                                                                    cagcaATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGAt                                     <  1:104558/140‑1 (MQ=255)

                                                                                    cagcaATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGAt                                     <  2:362783/140‑1 (MQ=255)

                                                                                    cagcaATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGAt                                     <  2:101852/140‑1 (MQ=255)

                                                                                             ttGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCAt                                              <  1:29385/122‑1 (MQ=255)

                                                                                             ttGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCAt                                              >  2:29385/1‑122 (MQ=255)

                                                                                              tgtgTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACttt                                >  2:359031/1‑135 (MQ=255)

                                                                                              tgtgTTCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACttt                                <  1:359031/135‑1 (MQ=255)

                                                                                                  ttCCTGCCTGCGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGa                       <  2:846056/140‑1 (MQ=255)

                                                                                                     ctgcctgcGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGAcgc                    >  1:847518/1‑140 (MQ=255)

                                                                                                         ctgcGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGa                <  2:368061/140‑1 (MQ=255)

                                                                                                         ctgcGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGa                <  1:252023/140‑1 (MQ=255)

                                                                                                         ctgcGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGa                <  2:74616/140‑1 (MQ=255)

                                                                                                         ctgcGCCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGa                <  2:755175/140‑1 (MQ=255)

                                                                                                            cgcCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCg             >  2:569303/1‑140 (MQ=255)

                                                                                                            cgcCAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCg             >  1:937201/1‑140 (MQ=255)

                                                                                                              ccAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGGCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTc           >  2:627735/1‑140 (MQ=255)

                                                                                                               cAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTg                        >  1:71431/1‑126 (MQ=255)

                                                                                                               cAGCTCGCCATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTg                        <  2:71431/126‑1 (MQ=255)

                                                                                                                       cATATTGCGTGCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAgc  >  1:212775/1‑140 (MQ=255)

CGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGC  >  NC_000913/1075274‑1075532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A