Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,348,715	A→G	S66S (AGT→AGC)	rnb ←	RNase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,348,715	0	A	G	100.0%	147.0 / NA	40	S66S (AGT→AGC)	rnb	RNase II
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/14); total (26/14)

CAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCACTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAG  >  NC_000913/1348578‑1348848

cAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCt                                                                                                                                     >  1:264884/1‑140 (MQ=255)

                    gagTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTc                                                                                                                 >  2:846524/1‑140 (MQ=255)

                     agTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCg                                                                                                                <  2:264884/140‑1 (MQ=255)

                     agTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCg                                                                                                                <  1:846524/140‑1 (MQ=255)

                        ggATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTc                                                                                                             >  2:553848/1‑140 (MQ=255)

                         gatgatCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCg                                                                                                            >  2:481331/1‑140 (MQ=255)

                         gatgatCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCg                                                                                                            >  1:160607/1‑140 (MQ=255)

                         gatgatCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCg                                                                                                            <  1:481331/140‑1 (MQ=255)

                                aGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCcatgca                                                                                                     <  1:1013583/140‑1 (MQ=255)

                                       aTGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACttt                                                                                              >  1:897552/1‑140 (MQ=255)

                                       aTGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACtt                                                                                               >  2:828114/1‑139 (MQ=255)

                                       aTGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACtt                                                                                               <  1:828114/139‑1 (MQ=255)

                                         ggCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACttttt                                                                                            >  1:568440/1‑140 (MQ=255)

                                                      tttttGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTgcggcgg                                                                               <  1:553848/140‑1 (MQ=255)

                                                         ttGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGcggcggcgg                                                                            >  1:154668/1‑140 (MQ=255)

                                                                 aaCCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAAt                                                                    >  2:970558/1‑140 (MQ=255)

                                                                             gAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTgcg                                                        >  2:305005/1‑140 (MQ=255)

                                                                             gAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTgcg                                                        >  2:252334/1‑140 (MQ=255)

                                                                                aCGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGt                                                     <  2:160607/140‑1 (MQ=255)

                                                                                 cGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTc                                                    <  2:314956/140‑1 (MQ=255)

                                                                                              gTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAg                                       >  2:939029/1‑140 (MQ=255)

                                                                                              gTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAg                                       >  1:843113/1‑140 (MQ=255)

                                                                                               ttCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGTAATAACTTTTTTGCGCGTCGACTTCCAGGAAGc                                      >  1:140355/1‑140 (MQ=255)

                                                                                                 cAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCa                                    <  1:305005/140‑1 (MQ=255)

                                                                                                   aCCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCaaa                                  >  1:11497/1‑140 (MQ=255)

                                                                                                        ttcttcTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCtt                             >  2:271669/1‑140 (MQ=255)

                                                                                                        ttcttcTGGCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTATTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCtt                             >  2:656187/1‑140 (MQ=255)

                                                                                                                gCTCTGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCtgtg                     >  1:316398/1‑140 (MQ=255)

                                                                                                                  tctGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGc                   >  1:899425/1‑140 (MQ=255)

                                                                                                                  tctGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGc                   >  1:775763/1‑140 (MQ=255)

                                                                                                                   ctGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCt                  <  2:430102/140‑1 (MQ=255)

                                                                                                                   ctGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCt                  >  2:900180/1‑140 (MQ=255)

                                                                                                                    tGCGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAAAGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCtt                 >  2:849739/1‑140 (MQ=255)

                                                                                                                      cGGATTCACGTTCTTTTTTGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCtttt               >  1:252901/1‑140 (MQ=255)

                                                                                                                      cGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCtttt               >  1:405941/1‑140 (MQ=255)

                                                                                                                      cGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCt                  >  2:21601/1‑137 (MQ=255)

                                                                                                                      cGGATTCACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCt                  <  1:21601/137‑1 (MQ=255)

                                                                                                                            cACGTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTaccacc         <  2:405941/140‑1 (MQ=255)

                                                                                                                               gTTCTTTTTCGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCt      <  1:851461/140‑1 (MQ=255)

                                                                                                                                   ttttttGCTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAg  <  2:252901/140‑1 (MQ=255)

CAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCTTCTGGCTCTGCGGATTCACGTTCTTTTTCACTGTGGATCACCGCGATAATTCGGTCGCCATGCATGACTTTTTTCATCTGCGGCGGCGGAATGAAATAACTTTTTTGCGCGTCGACTTCCAGGAAGCCAAAGCCTTTTTCTGTGGCTTTTACCACCCCTTCAG  >  NC_000913/1348578‑1348848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A