Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 138 62.6 2119710 99.4% 2106991 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,523,635 A→C N110T (AAC→ACC)  yncE → PQQ‑like domain‑containing protein YncE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,523,6350AC100.0% 187.9 / NA 50N110T (AAC→ACC) yncEPQQ‑like domain‑containing protein YncE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (32/18);  total (32/18)

CTGGATAAAGGTGGCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAAACAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGATGCCACGAACAC  >  NC_000913/1523499‑1523767
                                                                                                                                        |                                                                                                                                    
cTGGATAAAGGTGGCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAg                                                                                                                                   <  2:756823/140‑1 (MQ=255)
  ggATAAAGGTGGCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAgcg                                                                                                                                 <  2:86639/140‑1 (MQ=255)
  ggATAAAGGTGGCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAgcg                                                                                                                                 <  2:650347/140‑1 (MQ=255)
      aaaGGTGGCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGt                                                                                                                             <  1:727905/140‑1 (MQ=255)
             gCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCg                                                                                                                      >  1:537607/1‑140 (MQ=255)
                tGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATa                                                                                                                   <  2:537607/140‑1 (MQ=255)
                  gTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGa                                                                                                                 >  1:429819/1‑140 (MQ=255)
                            ttGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGATGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACg                                                                                                       >  2:293008/1‑140 (MQ=255)
                            ttGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACg                                                                                                       >  2:652160/1‑140 (MQ=255)
                                tCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCg                                                                                                   <  1:1018240/140‑1 (MQ=255)
                                    gTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGt                                                                                               >  2:42965/1‑140 (MQ=255)
                                         tctGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAg                                                                                          >  1:126141/1‑140 (MQ=255)
                                           tGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGc                                                                                        >  2:972891/1‑140 (MQ=255)
                                            gcaaGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGcc                                                                                       <  2:981872/138‑1 (MQ=255)
                                             gAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCg                                                                                      >  2:817844/1‑140 (MQ=255)
                                                   aCGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGt                                                                                >  1:39433/1‑140 (MQ=255)
                                                     gCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGc                                                                              >  1:688088/1‑140 (MQ=255)
                                                        ggCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTgg                                                                           <  2:373027/140‑1 (MQ=255)
                                                                aTAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGATGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGt                                                                   <  1:293008/140‑1 (MQ=255)
                                                                       tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGt                                                                   <  2:320438/133‑1 (MQ=255)
                                                                       tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGt                                                                   >  1:320438/1‑133 (MQ=255)
                                                                       tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            <  2:429819/140‑1 (MQ=255)
                                                                       tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            >  1:206576/1‑140 (MQ=255)
                                                                       tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            >  1:661561/1‑140 (MQ=255)
                                                                       tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            >  1:360411/1‑140 (MQ=255)
                                                                       tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            >  2:202157/1‑140 (MQ=255)
                                                                       tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            >  2:181297/1‑140 (MQ=255)
                                                                       tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGCTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            >  2:131340/1‑140 (MQ=255)
                                                                         tcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACg                                                          >  1:644349/1‑140 (MQ=255)
                                                                                 tttGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGt                                                  >  1:385738/1‑140 (MQ=255)
                                                                                       gCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGccc                                            >  2:1004113/1‑140 (MQ=255)
                                                                                                      acGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGa                             >  2:531849/1‑140 (MQ=255)
                                                                                                       cGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAg                            >  1:21811/1‑140 (MQ=255)
                                                                                                       cGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAg                            >  1:382583/1‑140 (MQ=255)
                                                                                                       cGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAg                            >  2:436824/1‑140 (MQ=255)
                                                                                                        gACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAGCCGCGTGAGc                           <  2:126141/140‑1 (MQ=255)
                                                                                                        gACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGc                           >  2:733700/1‑140 (MQ=255)
                                                                                                        gACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGc                           >  1:302156/1‑140 (MQ=255)
                                                                                                          cTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTg                         >  2:372165/1‑140 (MQ=255)
                                                                                                          cTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTg                         >  2:827/1‑140 (MQ=255)
                                                                                                          cTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTg                         >  1:612474/1‑140 (MQ=255)
                                                                                                            cAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGt                       >  1:14217/1‑140 (MQ=255)
                                                                                                                  ttGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGa                 <  1:652160/140‑1 (MQ=255)
                                                                                                                    gtgGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGt                               >  2:379453/1‑124 (MQ=255)
                                                                                                                    gtgGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGt                               <  1:379453/124‑1 (MQ=255)
                                                                                                                      ggTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGAt             <  1:202157/140‑1 (MQ=255)
                                                                                                                              aaCACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGATGCCACGaa     <  2:433049/140‑1 (MQ=255)
                                                                                                                              aaCACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGATGCCACGaa     <  1:131340/140‑1 (MQ=255)
                                                                                                                                cacCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGATGCCACGAaca   <  1:817844/140‑1 (MQ=255)
                                                                                                                                 acCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGATGCCACGAacac  >  1:311589/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                    
CTGGATAAAGGTGGCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAAACAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGATGCCACGAACAC  >  NC_000913/1523499‑1523767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A