Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 138 62.6 2119710 99.4% 2106991 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,603,730 A→G T238A (ACC→GCC)  lsrC → Autoinducer‑2 ABC transporter membrane subunit LsrC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,603,7300AG100.0% 66.2 / NA 19T238A (ACC→GCC) lsrCAutoinducer‑2 ABC transporter membrane subunit LsrC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/16);  total (3/16)

ACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTT  >  NC_000913/1603597‑1603863
                                                                                                                                     |                                                                                                                                     
aCAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCCGATTGGGTTTATTCCCAACCAAACCGGGGCCgggg                                                                                                                                 >  2:334380/1‑139 (MQ=255)
    ggCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTTCTTCGCCGATTTGGTTTTTCCCCCACCAGACCGGGGCCGGGCTggg                                                                                                                             >  1:92577/1‑139 (MQ=255)
            tCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAg                                                                                                                     <  2:621644/140‑1 (MQ=255)
            tCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAg                                                                                                                     <  2:311213/140‑1 (MQ=255)
                   ggCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTGCTTCGCAGATTGGTTTTTTCCCCCACCAGACCCGGGCCGGGGTGGGGATGAAAGCAAATGc                                                                                                              >  2:597403/1‑140 (MQ=255)
                        tCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCt                                                                                                         <  2:414166/140‑1 (MQ=255)
                                aaGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGTTTCGCCGATTGGTTTTTTCCCCCACCAGACCGGGGCCGGGGTGGGGATGAAAGCAATTGCAGCCTGCGTGCTg                                                                                                 >  2:644096/1‑140 (MQ=255)
                                 aGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTgg                                                                                                <  2:708700/140‑1 (MQ=255)
                                      ttCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTTCTTCGCAGATTGGTTTTTTTCCCCACCAGACCGGTGCCGGGGTGGAGGTGGAAGGAATTGTAGCCCGCGGGGTGggcggg                                                                                           >  2:205708/1‑139 (MQ=255)
                                      ttCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGggcggc                                                                                           <  1:205708/140‑1 (MQ=255)
                                           aTTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAg                                                                                      <  2:8028/140‑1 (MQ=255)
                                            ttGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTGCTTCGCAGGATGGGTTTATTCCCCACCACACCCGGGCCGGGGTGGAGATGGAAGCAAATGCAAGCTGCGTGCCGGGCGGCCTTAgg                                                                                     >  2:633201/1‑139 (MQ=255)
                                               tgGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGGGCCGGGCTGGGGATGAAAGCAATTTCAGCCTGCGTGCTGGGCGGCATTAGtttt                                                                                  >  2:745650/1‑139 (MQ=255)
                                                              aCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGt                                                                   <  1:934120/140‑1 (MQ=255)
                                                              aCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGt                                                                   <  1:558142/140‑1 (MQ=255)
                                                                       tGGCGGCACTGGCGGGAATTGTGTTTTCTTCGCAGATTTGTTTTTTCCCCCACCAGACCGGGGCCGGGGTGGGGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGGATTTGTTTGCTCGGTGGGTCCGGGGCGGTCAtt                                                          >  2:551490/1‑140 (MQ=255)
                                                                        ggcggcACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTg                                                         <  2:450983/140‑1 (MQ=255)
                                                                                  gCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACt                                               <  2:516488/140‑1 (MQ=255)
                                                                                  gCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACt                                               <  1:334380/140‑1 (MQ=255)
                                                                                     ggAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCgg                                            <  1:551490/140‑1 (MQ=255)
                                                                                             gtTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGt                                    >  1:1010488/1‑140 (MQ=255)
                                                                                                        aGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAg                         <  1:767653/140‑1 (MQ=255)
                                                                                                             ggTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGa                    <  1:597403/140‑1 (MQ=255)
                                                                                                               ttttATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGTTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATa                  >  1:679677/1‑140 (MQ=255)
                                                                                                                     ccccAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTAc            <  2:92577/140‑1 (MQ=255)
                                                                                                                          aCCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTg       <  1:603328/140‑1 (MQ=255)
                                                                                                                               aCCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt  >  2:85330/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                     
ACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTT  >  NC_000913/1603597‑1603863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A