Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,853,409	A→G	F191L (TTT→CTT)	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,853,409	0	A	G	100.0%	154.2 / NA	42	F191L (TTT→CTT)	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/20); total (22/20)

AGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGG  >  NC_000913/1853272‑1853537

aGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTACACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGcc                                                                                                                                >  2:828644/1‑140 (MQ=255)

    aGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGc                                                                                                                            >  1:776188/1‑140 (MQ=255)

     gCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGTCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCt                                                                                                                           <  2:323306/140‑1 (MQ=255)

     gCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCt                                                                                                                           <  2:266737/140‑1 (MQ=255)

     gCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCt                                                                                                                           <  1:668568/140‑1 (MQ=255)

       gggggTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTaa                                                                                                                         <  1:350829/140‑1 (MQ=255)

          ggTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAg                                                                                                                      >  2:984694/1‑140 (MQ=255)

          ggTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAg                                                                                                                      <  2:347930/140‑1 (MQ=255)

          ggTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAg                                                                                                                      <  2:776188/140‑1 (MQ=255)

           gTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGt                                                                                                                     <  1:70166/140‑1 (MQ=255)

                cGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGcc                                                                                                                >  1:993793/1‑140 (MQ=255)

                  aaTACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCaa                                                                                                              <  1:975085/140‑1 (MQ=255)

                    tACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATa                                                                                                            <  1:212030/140‑1 (MQ=255)

                    tACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATa                                                                                                            <  2:485209/140‑1 (MQ=255)

                    tACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATa                                                                                                            <  2:550669/140‑1 (MQ=255)

                        cttctCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATaccac                                                                                                        <  2:993793/140‑1 (MQ=255)

                         ttctCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATaccacc                                                                                                       <  1:830058/140‑1 (MQ=255)

                         ttctCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATaccacc                                                                                                       >  1:769647/1‑140 (MQ=255)

                         ttctCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATaccacc                                                                                                       >  1:697558/1‑140 (MQ=255)

                         ttctCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATaccacc                                                                                                       >  2:360078/1‑140 (MQ=255)

                          tctctcTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCAccc                                                                                                      <  2:94120/140‑1 (MQ=255)

                               ctTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCcagcag                                                                                                 >  2:189137/1‑140 (MQ=255)

                                 ttCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGaa                                                                                               >  1:610693/1‑140 (MQ=255)

                                   cAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCCAACAGTATGCCAATACCACCCAGCAGAAAc                                                                                             >  2:757344/1‑140 (MQ=255)

                                                    tCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTaaa                                                                            >  2:1049722/1‑140 (MQ=255)

                                                         aaGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAg                                                                       <  2:610693/140‑1 (MQ=255)

                                                             tGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGAt                                                                   <  2:598367/140‑1 (MQ=255)

                                                                           tGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTa                                                     <  1:708312/140‑1 (MQ=255)

                                                                            gCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTAt                                                    >  1:579995/1‑140 (MQ=255)

                                                                             cGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATc                                                   >  1:1000192/1‑140 (MQ=255)

                                                                             cGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATc                                                   >  2:1048885/1‑140 (MQ=255)

                                                                             cGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATc                                                   >  2:497735/1‑140 (MQ=255)

                                                                                 ttGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATcgccg                                               <  1:202929/140‑1 (MQ=255)

                                                                                 ttGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATcgccg                                               <  1:670131/140‑1 (MQ=255)

                                                                                         tCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGc                                       >  2:1059371/1‑140 (MQ=255)

                                                                                          cccTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCa                                      >  2:174475/1‑140 (MQ=255)

                                                                                              gccagccaTCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATggg                                  >  1:918672/1‑140 (MQ=255)

                                                                                                       cGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGtt                         >  2:675846/1‑140 (MQ=255)

                                                                                                         tGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTAc                       <  1:984694/140‑1 (MQ=255)

                                                                                                             gACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACcaac                   >  2:1032006/1‑140 (MQ=255)

                                                                                                              aCTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACcaaca                  >  2:728241/1‑140 (MQ=255)

                                                                                                                              aCCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCgg  >  1:883493/1‑140 (MQ=255)

AGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGG  >  NC_000913/1853272‑1853537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A