Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,875,472	T→C	V35V (GTA→GTG)	nimR ←	DNA‑binding transcriptional repressor NimR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,875,472	0	T	C	100.0%	179.2 / NA	48	V35V (GTA→GTG)	nimR	DNA‑binding transcriptional repressor NimR
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/23); total (25/23)

TACTTGATTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGTACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACTTAGCGATAATTTGTC  >  NC_000913/1875337‑1875603

tACTTGATTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAg                                                                                                                                 >  2:14114/1‑140 (MQ=255)

 aCTTGATTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAgg                                                                                                                                <  1:538148/140‑1 (MQ=255)

  cTTGATTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGa                                                                                                                               <  2:114477/140‑1 (MQ=255)

  cTTGATTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGa                                                                                                                               <  1:247261/140‑1 (MQ=255)

      aTTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGa                                                                                                                               >  2:95378/1‑136 (MQ=255)

      aTTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGa                                                                                                                               <  1:95378/136‑1 (MQ=255)

      aTTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTgg                                                                                                                           <  2:846734/140‑1 (MQ=255)

         gCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTa                                                                                                                        >  2:1039342/1‑140 (MQ=255)

               ttCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCAt                                                                                                                  <  2:639935/140‑1 (MQ=255)

               ttCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCAt                                                                                                                  <  1:14114/140‑1 (MQ=255)

                tCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATc                                                                                                                 >  1:91747/1‑140 (MQ=255)

                    ccccGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGa                                                                                                                               <  2:211607/122‑1 (MQ=255)

                    ccccGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGa                                                                                                                               >  1:211607/1‑122 (MQ=255)

                    ccccGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGtt                                                                                                             <  1:233858/140‑1 (MQ=255)

                        gcccgGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCgg                                                                                                         <  1:560980/140‑1 (MQ=255)

                                        gCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGc                                                                                         >  1:89780/1‑140 (MQ=255)

                                          atatTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAGCTCATCCGTTCCGGCGTGAATACAAAATGCGa                                                                                       >  1:341275/1‑140 (MQ=255)

                                             ttGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACcg                                                                                    <  2:823429/140‑1 (MQ=255)

                                               gtgGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACcgcg                                                                                  <  1:1039342/140‑1 (MQ=255)

                                               gtgGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACcgcg                                                                                  <  1:750300/140‑1 (MQ=255)

                                                tgGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCgg                                                                                 >  1:228960/1‑140 (MQ=255)

                                                 ggcggcACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGc                                                                                >  2:772908/1‑140 (MQ=255)

                                                                aaaGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCGGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTgtcggtc                                                                 <  2:802885/140‑1 (MQ=255)

                                                                  aGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGgtgt                                                                      <  1:664544/133‑1 (MQ=255)

                                                                  aGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGtcggtcgg                                                               >  1:231370/1‑140 (MQ=255)

                                                                  aGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCATTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGgtgt                                                                      >  2:664544/1‑133 (MQ=255)

                                                                      ttttCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTc                                                           >  2:280461/1‑140 (MQ=255)

                                                                       tttCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCa                                                          <  2:89780/140‑1 (MQ=255)

                                                                          ccACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAg                                                       >  1:494214/1‑140 (MQ=255)

                                                                           cACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGc                                                      <  2:414969/140‑1 (MQ=255)

                                                                              cGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCAt                                                   <  2:494214/140‑1 (MQ=255)

                                                                               gTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCAtt                                                  >  1:459962/1‑140 (MQ=255)

                                                                                tACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTg                                                 >  2:324455/1‑140 (MQ=255)

                                                                                tACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTg                                                 >  2:693744/1‑140 (MQ=255)

                                                                                        aTTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCaa                                         >  1:700056/1‑140 (MQ=255)

                                                                                         ttGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAAc                                        <  2:91747/140‑1 (MQ=255)

                                                                                          tGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTgaga                                              >  1:459316/1‑133 (MQ=255)

                                                                                          tGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTgaga                                              <  2:459316/133‑1 (MQ=255)

                                                                                          tGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAAcc                                       >  2:535942/1‑140 (MQ=255)

                                                                                                 tGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGcat                                >  2:17263/1‑140 (MQ=255)

                                                                                                     gcgcCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCAtt                            <  2:228960/140‑1 (MQ=255)

                                                                                                           gTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTcc                      >  2:416439/1‑140 (MQ=255)

                                                                                                            tATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCg                     >  1:956677/1‑140 (MQ=255)

                                                                                                                aGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACt                 >  1:444820/1‑140 (MQ=255)

                                                                                                                    gTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACTTAGc             <  1:416439/140‑1 (MQ=255)

                                                                                                                          tGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACTTAGCGATAAt       >  2:539363/1‑140 (MQ=255)

                                                                                                                             ggTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACTTAGCGATAATTTg    <  2:622721/140‑1 (MQ=255)

                                                                                                                               tGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACTTAGCGATAATTTGTc  >  2:368055/1‑140 (MQ=255)

TACTTGATTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGTACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACTTAGCGATAATTTGTC  >  NC_000913/1875337‑1875603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A