Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,408,070	T→C	R170R (CGT→CGC)	alaA →	glutamate‑‑pyruvate aminotransferase AlaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,408,070	0	T	C	100.0%	158.5 / NA	43	R170R (CGT→CGC)	alaA	glutamate‑‑pyruvate aminotransferase AlaA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/27); total (16/27)

CTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTCTCT  >  NC_000913/2407934‑2408203

cTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCggg                                                                                                                                    <  1:812864/140‑1 (MQ=255)

cTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCggg                                                                                                                                    <  1:520293/140‑1 (MQ=255)

 tGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGa                                                                                                                                   <  1:624439/140‑1 (MQ=255)

 tGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGa                                                                                                                                   <  2:795526/140‑1 (MQ=255)

 tGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGa                                                                                                                                   >  1:451188/1‑140 (MQ=255)

 tGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGa                                                                                                                                   <  1:598247/140‑1 (MQ=255)

      cAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGtt                                                                                                                              <  1:989974/140‑1 (MQ=255)

       aGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGtta                                                                                                                             >  1:605572/1‑140 (MQ=255)

           tACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTtattat                                                                                                                         <  2:605572/140‑1 (MQ=255)

             cccACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCa                                                                                                                       <  1:602404/140‑1 (MQ=255)

              ccACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCaa                                                                                                                      <  2:529311/140‑1 (MQ=255)

                  tctGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCa                                                                                                                  >  2:483370/1‑140 (MQ=255)

                   ctGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTtattat                                                                                                                         <  1:542533/132‑1 (MQ=255)

                   ctGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTtattat                                                                                                                         >  2:542533/1‑132 (MQ=255)

                       aCCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATaa                                                                                                             >  1:692847/1‑140 (MQ=255)

                       aCCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATaa                                                                                                             >  1:551451/1‑140 (MQ=255)

                           cggcggTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCa                                                                                                         >  1:749376/1‑140 (MQ=255)

                           cggcggTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCa                                                                                                         >  2:212014/1‑140 (MQ=255)

                             gcggTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAAc                                                                                                       <  1:212014/140‑1 (MQ=255)

                                 tttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgc                                                                                                   <  1:178138/140‑1 (MQ=255)

                                  ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgcg                                                                                                  <  1:592320/140‑1 (MQ=255)

                                  ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgcg                                                                                                  <  2:749376/140‑1 (MQ=255)

                                  ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgcg                                                                                                  <  1:818085/140‑1 (MQ=255)

                                  ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgcg                                                                                                  <  1:483370/140‑1 (MQ=255)

                                    cGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGcgcg                                                                                                <  1:752046/140‑1 (MQ=255)

                                                   aaGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgcg                                                                                                  <  1:651507/123‑1 (MQ=255)

                                                   aaGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgcg                                                                                                  >  2:651507/1‑123 (MQ=255)

                                                   aaGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAagag                                                                                 >  2:287531/1‑140 (MQ=255)

                                                             ttATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtgg                                                                       <  1:527728/140‑1 (MQ=255)

                                                             ttATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtgg                                                                       <  1:444986/140‑1 (MQ=255)

                                                                     gCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtggagattgtg                                                               <  2:1013206/140‑1 (MQ=255)

                                                                       gATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCACTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtggagattgtgga                                                             <  2:988845/140‑1 (MQ=255)

                                                                            aTCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtggagattgtggagattg                                                        <  1:724054/140‑1 (MQ=255)

                                                                            aTCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtggagattgtggagattg                                                        <  1:369678/140‑1 (MQ=255)

                                                                                 ctGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGt                                                   <  2:551451/140‑1 (MQ=255)

                                                                                   gACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCa                                                 >  2:269120/1‑140 (MQ=255)

                                                                                    aCTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCaa                                                <  1:269120/140‑2 (MQ=255)

                                                                                        gTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATa                                            >  1:587757/1‑140 (MQ=255)

                                                                                        gTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATa                                            >  1:483901/1‑140 (MQ=255)

                                                                                                          tatTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCg                          >  1:724979/1‑140 (MQ=255)

                                                                                                                                cGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACa           <  1:964465/133‑1 (MQ=255)

                                                                                                                                cGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACa           >  2:964465/1‑133 (MQ=255)

                                                                                                                                  tACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTctct  >  2:932942/1‑140 (MQ=255)

CTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTCTCT  >  NC_000913/2407934‑2408203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A