Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,816,960	C→T	A182T (GCC→ACC)	yqaB ←	fructose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,816,960	0	C	T	100.0%	174.6 / NA	50	A182T (GCC→ACC)	yqaB	fructose‑1‑phosphatase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/21); total (29/21)

ATGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGCGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACG  >  NC_000913/2816824‑2817093

aTGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTc                                                                                                                                    <  1:329500/140‑1 (MQ=255)

   aCTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCAt                                                                                                                                 >  2:22452/1‑140 (MQ=255)

   aCTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCAt                                                                                                                                 <  1:134388/140‑1 (MQ=255)

   aCTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCAt                                                                                                                                 >  1:720059/1‑140 (MQ=255)

   aCTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCAt                                                                                                                                 >  2:70334/1‑140 (MQ=255)

             gAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCa                                                                                                                       >  2:646821/1‑140 (MQ=255)

             gAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCa                                                                                                                       <  1:646821/140‑1 (MQ=255)

             gAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCa                                                                                                                       >  1:613284/1‑140 (MQ=255)

             gAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGAGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCa                                                                                                                       >  2:593290/1‑140 (MQ=255)

              aaaGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCAc                                                                                                                      >  1:275600/1‑140 (MQ=255)

                   aaCATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAAGGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGgcc                                                                                                                 >  2:737797/1‑140 (MQ=255)

                                             gctGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTc                                                                                       <  2:720059/138‑1 (MQ=255)

                                              ttGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCa                                                                                      <  1:70334/140‑1 (MQ=255)

                                               tGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCaa                                                                                     <  1:904030/140‑1 (MQ=255)

                                               tGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCaa                                                                                     <  2:378423/140‑1 (MQ=255)

                                               tGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCaa                                                                                     <  1:560497/140‑1 (MQ=255)

                                               tGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCaa                                                                                     <  2:86915/140‑1 (MQ=255)

                                                 ccGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAg                                                                                   <  2:833488/140‑1 (MQ=255)

                                                 ccGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAg                                                                                   >  1:1030709/1‑140 (MQ=255)

                                                    ggTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCAGTCACAGCAAGCGAACATACACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGAcc                                                                                <  2:948179/140‑1 (MQ=255)

                                                              tAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCg                                                                      >  2:15362/1‑140 (MQ=255)

                                                               aGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGt                                                                     >  1:173142/1‑140 (MQ=255)

                                                               aGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATACCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGt                                                                     >  2:371477/1‑140 (MQ=255)

                                                                gcgcTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTc                                                                    >  2:450559/1‑140 (MQ=255)

                                                                gcgcTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTc                                                                    >  1:959982/1‑140 (MQ=255)

                                                                gcgcTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTc                                                                    >  1:511315/1‑140 (MQ=255)

                                                                gcgcTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATACCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTc                                                                    <  1:371477/140‑1 (MQ=255)

                                                                 cgcTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCg                                                                   >  1:877355/1‑140 (MQ=255)

                                                                 cgcTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCg                                                                   >  2:537282/1‑140 (MQ=255)

                                                                    tGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGtt                                                                >  2:680760/1‑140 (MQ=255)

                                                                      agaAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGc                                                              >  1:835231/1‑140 (MQ=255)

                                                                       gaAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCa                                                             <  1:145284/140‑1 (MQ=255)

                                                                       gaAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCa                                                             <  1:647738/140‑1 (MQ=255)

                                                                       gaAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCa                                                             <  1:450559/140‑1 (MQ=255)

                                                                                tGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATAc                                                    <  2:613284/140‑1 (MQ=255)

                                                                                            agaAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGcaca                                        >  2:814697/1‑140 (MQ=255)

                                                                                              aaaaGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCacaac                                      <  1:15362/140‑1 (MQ=255)

                                                                                                            ttCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTgg                        >  2:141236/1‑140 (MQ=255)

                                                                                                                   acaGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGACATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGt                 <  1:792975/140‑1 (MQ=255)

                                                                                                                     aGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGttt               >  2:860961/1‑140 (MQ=255)

                                                                                                                      gCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTa              <  2:511315/140‑1 (MQ=255)

                                                                                                                         aGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATgg           >  2:251596/1‑140 (MQ=255)

                                                                                                                          gCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTAt             >  2:818080/1‑137 (MQ=255)

                                                                                                                          gCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTAt             <  1:818080/137‑1 (MQ=255)

                                                                                                                             aaCATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGgcg                      <  1:324993/125‑1 (MQ=255)

                                                                                                                             aaCATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGgcg                      >  2:324993/1‑125 (MQ=255)

                                                                                                                             aaCATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGtt       >  1:596593/1‑140 (MQ=255)

                                                                                                                               cATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTg     >  1:167463/1‑140 (MQ=255)

                                                                                                                               cATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTg     >  2:909529/1‑140 (MQ=255)

                                                                                                                                  ccACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACg  >  1:1055841/1‑140 (MQ=255)

ATGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGCGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACG  >  NC_000913/2816824‑2817093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A