Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F28 I1 R1
|
138 |
62.6 |
2119710 |
99.4% |
2106991 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,062,332 |
G→A |
A495T (GCC→ACC) |
scpA → |
methylmalonyl‑CoA mutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,062,332 | 0 | G | A | 100.0%
| 78.1
/ NA
| 23 | A495T (GCC→ACC) | scpA | methylmalonyl‑CoA mutase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/0); total (23/0) |
CGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCG > NC_000913/3062200‑3062461
|
cGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGt > 1:1017499/1‑140 (MQ=255)
cGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGt > 1:967812/1‑140 (MQ=255)
tCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAAcc > 2:330676/1‑140 (MQ=255)
tCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAAcc > 1:276401/1‑140 (MQ=255)
tCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAAcc > 1:859542/1‑140 (MQ=255)
tgtCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGa > 1:217711/1‑140 (MQ=255)
tgtCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGa > 1:297223/1‑140 (MQ=255)
tCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAAc > 2:157523/1‑140 (MQ=255)
aacaaGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGc > 2:736119/1‑140 (MQ=255)
caaGTACAAACTGGATCACGAAGACGAAACCGATGTATTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGccc > 1:703324/1‑140 (MQ=255)
aaaCGGGACCAAAAAGACAAACCCGTTGTTTTGGAGACCGAAAGGGTGGGGGTGCGAAAGGAAAAATTTGCTCCGCGGAAACGCATCCGCCCCCCCGGTGATGATCCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCa < 2:823362/140‑1 (MQ=255)
gATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcgccgc > 1:257403/1‑140 (MQ=255)
gAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCa > 1:499894/1‑140 (MQ=255)
aTCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGAAACGCATTCGCGCCACCCGTGAGGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGc > 1:926604/1‑140 (MQ=255)
aCAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGtt > 2:632244/1‑140 (MQ=255)
cGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATg > 2:541993/1‑140 (MQ=255)
cGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATg > 2:63114/1‑140 (MQ=255)
gTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGc > 2:103556/1‑140 (MQ=255)
tGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGcc > 1:263295/1‑140 (MQ=255)
gggTGCGAAACGACAAATTTGCTCCGCGGAACCGCTTCCGCCCCCCCGGTGATGATCCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCt < 1:309919/139‑1 (MQ=255)
tAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTc > 2:406502/1‑140 (MQ=255)
gCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCa > 2:11662/1‑140 (MQ=255)
gCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCa > 1:352415/1‑140 (MQ=255)
cTTCGCTGGAACGCATTCGCGCCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGt > 2:468/1‑140 (MQ=255)
cgcCACCCGTGATGATACCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTgg > 2:322226/1‑140 (MQ=255)
cgggaatgatcCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCg < 1:736119/135‑1 (MQ=255)
|
CGTGTCATCGTTGGTGTCAACAAGTACAAACTGGATCACGAAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCG > NC_000913/3062200‑3062461
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
